data_EMD-14647
# 
_entry.id   EMD-14647 
# 
_audit_conform.dict_name       mmcif_pdbx.dic 
_audit_conform.dict_version    5.381 
_audit_conform.dict_location   http://mmcif.pdb.org/dictionaries/ascii/mmcif_pdbx.dic 
# 
loop_
_database_2.database_id 
_database_2.database_code 
_database_2.pdbx_database_accession 
_database_2.pdbx_DOI 
WWPDB D_1292121996 ? ? 
EMDB  EMD-14647    ? ? 
# 
loop_
_pdbx_database_related.db_name 
_pdbx_database_related.details 
_pdbx_database_related.db_id 
_pdbx_database_related.content_type 
EMDB . EMD-14636 'other EM volume'      
PDB  . 7ZD5      unspecified            
EMDB . EMD-14647 'associated EM volume' 
# 
_citation.abstract                  ? 
_citation.abstract_id_CAS           ? 
_citation.book_id_ISBN              ? 
_citation.book_publisher            ? 
_citation.book_publisher_city       ? 
_citation.book_title                ? 
_citation.coordinate_linkage        ? 
_citation.country                   US 
_citation.database_id_Medline       ? 
_citation.details                   ? 
_citation.id                        primary 
_citation.journal_abbrev            Nat.Chem.Biol. 
_citation.journal_id_ASTM           ? 
_citation.journal_id_CSD            ? 
_citation.journal_id_ISSN           1552-4469 
_citation.journal_full              ? 
_citation.journal_issue             ? 
_citation.journal_volume            19 
_citation.language                  ? 
_citation.page_first                992 
_citation.page_last                 1003 
_citation.title                     'Dissecting the conformational complexity and mechanism of a bacterial heme transporter.' 
_citation.year                      2023 
_citation.database_id_CSD           ? 
_citation.pdbx_database_id_DOI      10.1038/s41589-023-01314-5 
_citation.pdbx_database_id_PubMed   37095238 
_citation.pdbx_database_id_patent   ? 
_citation.unpublished_flag          ? 
# 
loop_
_citation_author.citation_id 
_citation_author.name 
_citation_author.ordinal 
_citation_author.identifier_ORCID 
primary 'Wu, D.'            1  ?                   
primary 'Mehdipour, A.R.'   2  ?                   
primary 'Finke, F.'         3  ?                   
primary 'Goojani, H.G.'     4  ?                   
primary 'Groh, R.R.'        5  ?                   
primary 'Grund, T.N.'       6  ?                   
primary 'Reichhart, T.M.B.' 7  ?                   
primary 'Zimmermann, R.'    8  ?                   
primary 'Welsch, S.'        9  0000-0002-6049-6664 
primary 'Bald, D.'          10 ?                   
primary 'Shepherd, M.'      11 ?                   
primary 'Hummer, G.'        12 0000-0001-7768-746X 
primary 'Safarian, S.'      13 0000-0002-0232-1612 
# 
loop_
_entity.details 
_entity.formula_weight 
_entity.id 
_entity.src_method 
_entity.type 
_entity.pdbx_description 
_entity.pdbx_number_of_molecules 
_entity.pdbx_parent_entity_id 
_entity.pdbx_mutation 
_entity.pdbx_fragment 
_entity.pdbx_ec 
_entity.pdbx_modification 
_entity.pdbx_formula_weight_exptl 
_entity.pdbx_formula_weight_exptl_method 
_entity.pdbx_target_id 
_entity.pdbx_entities_per_biological_unit 
? ? 1 man polymer CydC ? ? ? ? ? ? ? ? ? ? 
? ? 2 man polymer CydD ? ? ? ? ? ? ? ? ? ? 
# 
loop_
_entity_poly.entity_id 
_entity_poly.type 
_entity_poly.pdbx_seq_one_letter_code 
1 'polypeptide(L)' 
;MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLSASAVAGVAGLYSFNYM
LPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSKLLPLSPAGLARYRQGELL
NRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDFTLAFTLGGIMLLTLFLMPPL
FYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGASDRYRTQLENTEIQWLEAQRRQ
SELTALSQAIMLLIGALAVILMLWMASGGVGGNAQPGALIALFVFCALAAFEALAPVTGA
FQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVADRVSLTLRDVQFTYPEQSQQALKGIS
LQVNAGEHIAILGRTGCGKSTLLQQLTRAWDPQQGEILLNDSPIASLNEAALRQTISVVP
QRVHLFSATLRDNLLLASPGSSDEALSEILRRVGLEKLLEDAGLNSWLGEGGRQLSGGEL
RRLAIARALLHDAPLVLLDEPTEGLDATTESQILELLAEMMREKTVLMVTHRLRGLSRFQ
QIIVMDNGQIIEQGTHAELLARQGRYYQFKQGL
;
2 'polypeptide(L)' 
;MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWFMARILQHMIMENIPRE
ALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQVLDRLQQAGPAWIQGKPAG
SWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFPSNWAAALILLGTAPLIPLFM
ALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIFGRGEAEIESIRSASEDFRQRTM
EVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGELDFGHYDTGVTLAAGFLALILAPEF
FQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAHPQRGEAELASTDPVTIEAEELFITSP
EGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSLLNALSGFLSYQGSLRINGIELRDLSPES
WRKHLSWVGQNPQLPAATLRDNVLLARPDASEQELQAALDNAWVSEFLPLLPQGVDTPVG
DQAARLSVGQAQRVAVARALLNPCSLLLLDEPAASLDAHSEQRVMEALNAASLRQTTLMV
THQLEDLADWDVIWVMQDGRIIEQGRYAELSVAGGPFATLLAHRQEEI
;
# 
loop_
_entity_src_gen.entity_id 
_entity_src_gen.gene_src_common_name 
_entity_src_gen.gene_src_details 
_entity_src_gen.gene_src_genus 
_entity_src_gen.gene_src_species 
_entity_src_gen.gene_src_strain 
_entity_src_gen.gene_src_tissue 
_entity_src_gen.gene_src_tissue_fraction 
_entity_src_gen.host_org_genus 
_entity_src_gen.host_org_species 
_entity_src_gen.pdbx_gene_src_fragment 
_entity_src_gen.pdbx_gene_src_gene 
_entity_src_gen.pdbx_gene_src_scientific_name 
_entity_src_gen.pdbx_gene_src_variant 
_entity_src_gen.pdbx_gene_src_cell_line 
_entity_src_gen.pdbx_gene_src_atcc 
_entity_src_gen.pdbx_gene_src_organ 
_entity_src_gen.pdbx_gene_src_organelle 
_entity_src_gen.pdbx_gene_src_plasmid 
_entity_src_gen.pdbx_gene_src_plasmid_name 
_entity_src_gen.pdbx_gene_src_cell 
_entity_src_gen.pdbx_gene_src_cellular_location 
_entity_src_gen.pdbx_host_org_gene 
_entity_src_gen.pdbx_host_org_organ 
_entity_src_gen.pdbx_host_org_organelle 
_entity_src_gen.pdbx_host_org_cellular_location 
_entity_src_gen.pdbx_host_org_strain 
_entity_src_gen.pdbx_host_org_tissue_fraction 
_entity_src_gen.pdbx_description 
_entity_src_gen.host_org_common_name 
_entity_src_gen.host_org_details 
_entity_src_gen.host_org_strain 
_entity_src_gen.plasmid_details 
_entity_src_gen.plasmid_name 
_entity_src_gen.pdbx_host_org_variant 
_entity_src_gen.pdbx_host_org_cell_line 
_entity_src_gen.pdbx_host_org_atcc 
_entity_src_gen.pdbx_host_org_culture_collection 
_entity_src_gen.pdbx_host_org_cell 
_entity_src_gen.pdbx_host_org_scientific_name 
_entity_src_gen.pdbx_host_org_tissue 
_entity_src_gen.pdbx_host_org_vector 
_entity_src_gen.pdbx_host_org_vector_type 
_entity_src_gen.expression_system_id 
_entity_src_gen.gene_src_dev_stage 
_entity_src_gen.start_construct_id 
_entity_src_gen.pdbx_gene_src_ncbi_taxonomy_id 
_entity_src_gen.pdbx_host_org_ncbi_taxonomy_id 
_entity_src_gen.pdbx_src_id 
_entity_src_gen.pdbx_alt_source_flag 
_entity_src_gen.pdbx_seq_type 
_entity_src_gen.pdbx_beg_seq_num 
_entity_src_gen.pdbx_end_seq_num 
_entity_src_gen.pdbx_gene_src_culture_collection 
1 ? ? ? ? ? ? ? ? ? ? cydc 'Escherichia coli K-12' ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? C43 ? ? ? ? 'Escherichia coli K-12' ? 
? ? ? ? ? 83333 83333 1 ? ? ? ? ? 
2 ? ? ? ? ? ? ? ? ? ? cydd 'Escherichia coli K-12' ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? C43 ? ? ? ? 'Escherichia coli K-12' ? 
? ? ? ? ? 83333 83333 1 ? ? ? ? ? 
# 
_exptl.entry_id         EMD-14647 
_exptl.details          ? 
_exptl.method           'ELECTRON MICROSCOPY' 
_exptl.method_details   ? 
# 
_struct_keywords.entry_id        EMD-14647 
_struct_keywords.text            'ABC transporter, CydDC, Heme transporter, MEMBRANE PROTEIN' 
_struct_keywords.pdbx_keywords   'MEMBRANE PROTEIN' 
_struct_keywords.pdbx_details    ? 
# 
_em_3d_fitting.entry_id          EMD-14647 
_em_3d_fitting.id                1 
_em_3d_fitting.details           ? 
_em_3d_fitting.overall_b_value   ? 
_em_3d_fitting.ref_protocol      ? 
_em_3d_fitting.ref_space         ? 
_em_3d_fitting.target_criteria   ? 
_em_3d_fitting.method            ? 
# 
_em_3d_reconstruction.entry_id                    EMD-14647 
_em_3d_reconstruction.id                          1 
_em_3d_reconstruction.algorithm                   ? 
_em_3d_reconstruction.details                     ? 
_em_3d_reconstruction.fsc_type                    'EVEN/ODD MAPS REFINED TOTALLY INDEPENDENT (GOLD STANDARD)' 
_em_3d_reconstruction.refinement_type             ? 
_em_3d_reconstruction.image_processing_id         1 
_em_3d_reconstruction.num_class_averages          ? 
_em_3d_reconstruction.num_particles               73885 
_em_3d_reconstruction.resolution                  3.49 
_em_3d_reconstruction.resolution_method           'FSC 0.143 CUT-OFF' 
_em_3d_reconstruction.symmetry_type               POINT 
_em_3d_reconstruction.method                      ? 
_em_3d_reconstruction.nominal_pixel_size          ? 
_em_3d_reconstruction.actual_pixel_size           ? 
_em_3d_reconstruction.magnification_calibration   ? 
# 
_em_buffer.id            1 
_em_buffer.details       ? 
_em_buffer.pH            6 
_em_buffer.specimen_id   1 
_em_buffer.name          ? 
# 
_em_entity_assembly.id                   1 
_em_entity_assembly.parent_id            0 
_em_entity_assembly.details              ? 
_em_entity_assembly.go_id                ? 
_em_entity_assembly.name                 'CydDC heterodimer' 
_em_entity_assembly.source               RECOMBINANT 
_em_entity_assembly.type                 COMPLEX 
_em_entity_assembly.chimera              NO 
_em_entity_assembly.entity_id_list       '1, 2' 
_em_entity_assembly.ipr_id               ? 
_em_entity_assembly.synonym              ? 
_em_entity_assembly.number_of_copies     ? 
_em_entity_assembly.oligomeric_details   ? 
# 
_em_imaging.id                              1 
_em_imaging.entry_id                        EMD-14647 
_em_imaging.accelerating_voltage            300 
_em_imaging.alignment_procedure             ? 
_em_imaging.c2_aperture_diameter            ? 
_em_imaging.calibrated_defocus_max          ? 
_em_imaging.calibrated_defocus_min          ? 
_em_imaging.calibrated_magnification        ? 
_em_imaging.cryogen                         ? 
_em_imaging.details                         ? 
_em_imaging.electron_source                 'FIELD EMISSION GUN' 
_em_imaging.illumination_mode               'FLOOD BEAM' 
_em_imaging.microscope_model                'TFS KRIOS' 
_em_imaging.mode                            'BRIGHT FIELD' 
_em_imaging.nominal_cs                      ? 
_em_imaging.nominal_defocus_max             2100 
_em_imaging.nominal_defocus_min             1100 
_em_imaging.nominal_magnification           ? 
_em_imaging.recording_temperature_maximum   ? 
_em_imaging.recording_temperature_minimum   ? 
_em_imaging.residual_tilt                   ? 
_em_imaging.specimen_holder_model           ? 
_em_imaging.specimen_id                     1 
_em_imaging.sample_support_id               1 
_em_imaging.citation_id                     ? 
_em_imaging.detector_id                     1 
_em_imaging.scans_id                        1 
_em_imaging.date                            ? 
_em_imaging.temperature                     ? 
_em_imaging.tilt_angle_min                  ? 
_em_imaging.tilt_angle_max                  ? 
_em_imaging.astigmatism                     ? 
_em_imaging.detector_distance               ? 
_em_imaging.electron_beam_tilt_params       ? 
_em_imaging.specimen_holder_type            ? 
# 
_em_sample_support.id               1 
_em_sample_support.specimen_id      1 
_em_sample_support.details          ? 
_em_sample_support.grid_material    COPPER 
_em_sample_support.grid_mesh_size   300 
_em_sample_support.grid_type        'Quantifoil R1.2/1.3' 
_em_sample_support.method           ? 
_em_sample_support.film_material    ? 
# 
_em_vitrification.id                      1 
_em_vitrification.specimen_id             1 
_em_vitrification.chamber_temperature     ? 
_em_vitrification.cryogen_name            ETHANE 
_em_vitrification.details                 ? 
_em_vitrification.humidity                100 
_em_vitrification.instrument              ? 
_em_vitrification.entry_id                EMD-14647 
_em_vitrification.sample_preparation_id   ? 
_em_vitrification.citation_id             ? 
_em_vitrification.method                  ? 
_em_vitrification.temp                    ? 
_em_vitrification.time_resolved_state     ? 
# 
_em_experiment.entry_id                EMD-14647 
_em_experiment.id                      1 
_em_experiment.aggregation_state       PARTICLE 
_em_experiment.reconstruction_method   'SINGLE PARTICLE' 
_em_experiment.entity_assembly_id      1 
# 
_em_admin.entry_id              EMD-14647 
_em_admin.current_status        REL 
_em_admin.deposition_date       2022-03-29 
_em_admin.deposition_site       PDBE 
_em_admin.details               ? 
_em_admin.last_update           2023-11-01 
_em_admin.map_release_date      2023-04-19 
_em_admin.obsoleted_date        ? 
_em_admin.title                 'IF(apo/asym) conformation of CydDC (Dataset-7)' 
_em_admin.header_release_date   2023-04-19 
# 
loop_
_em_author_list.ordinal 
_em_author_list.author 
_em_author_list.identifier_ORCID 
1 'Wu, D.'       0000-0003-1389-0199 
2 'Safarian, S.' 0000-0002-0232-1612 
# 
_em_ctf_correction.id                                1 
_em_ctf_correction.em_image_processing_id            1 
_em_ctf_correction.type                              'PHASE FLIPPING AND AMPLITUDE CORRECTION' 
_em_ctf_correction.details                           ? 
_em_ctf_correction.amplitude_correction              ? 
_em_ctf_correction.amplitude_correction_factor       ? 
_em_ctf_correction.amplitude_correction_space        ? 
_em_ctf_correction.correction_operation              ? 
_em_ctf_correction.phase_reversal                    ? 
_em_ctf_correction.phase_reversal_anisotropic        ? 
_em_ctf_correction.phase_reversal_correction_space   ? 
# 
_em_entity_assembly_molwt.entity_assembly_id   1 
_em_entity_assembly_molwt.id                   1 
_em_entity_assembly_molwt.experimental_flag    YES 
_em_entity_assembly_molwt.units                MEGADALTONS 
_em_entity_assembly_molwt.value                0.130 
# 
_em_entity_assembly_naturalsource.id                   2 
_em_entity_assembly_naturalsource.entity_assembly_id   1 
_em_entity_assembly_naturalsource.cell                 ? 
_em_entity_assembly_naturalsource.cellular_location    ? 
_em_entity_assembly_naturalsource.ncbi_tax_id          562 
_em_entity_assembly_naturalsource.organ                ? 
_em_entity_assembly_naturalsource.organelle            ? 
_em_entity_assembly_naturalsource.organism             'Escherichia coli' 
_em_entity_assembly_naturalsource.strain               K12 
_em_entity_assembly_naturalsource.tissue               ? 
# 
_em_entity_assembly_recombinant.id                   2 
_em_entity_assembly_recombinant.entity_assembly_id   1 
_em_entity_assembly_recombinant.cell                 ? 
_em_entity_assembly_recombinant.ncbi_tax_id          562 
_em_entity_assembly_recombinant.organism             'Escherichia coli' 
_em_entity_assembly_recombinant.plasmid              ? 
_em_entity_assembly_recombinant.strain               K12 
# 
loop_
_em_euler_angle_assignment.id 
_em_euler_angle_assignment.details 
_em_euler_angle_assignment.image_processing_id 
_em_euler_angle_assignment.order 
_em_euler_angle_assignment.proj_matching_angular_sampling 
_em_euler_angle_assignment.proj_matching_merit_function 
_em_euler_angle_assignment.proj_matching_num_projections 
_em_euler_angle_assignment.type 
1 ? 1 INITIAL ? ? ? 'MAXIMUM LIKELIHOOD'     
2 ? 1 FINAL   ? ? ? 'ANGULAR RECONSTITUTION' 
# 
_em_grid_pretreatment.id                  1 
_em_grid_pretreatment.sample_support_id   1 
_em_grid_pretreatment.atmosphere          ? 
_em_grid_pretreatment.pressure            ? 
_em_grid_pretreatment.time                90 
_em_grid_pretreatment.type                'GLOW DISCHARGE' 
# 
_em_image_processing.id                   1 
_em_image_processing.image_recording_id   1 
_em_image_processing.details              ? 
# 
_em_image_recording.id                                  1 
_em_image_recording.imaging_id                          1 
_em_image_recording.avg_electron_dose_per_image         40 
_em_image_recording.average_exposure_time               ? 
_em_image_recording.details                             ? 
_em_image_recording.detector_mode                       ? 
_em_image_recording.film_or_detector_model              'GATAN K3 BIOQUANTUM (6k x 4k)' 
_em_image_recording.num_diffraction_images              ? 
_em_image_recording.num_grids_imaged                    ? 
_em_image_recording.num_real_images                     ? 
_em_image_recording.avg_electron_dose_per_subtomogram   ? 
# 
loop_
_em_map.entry_id 
_em_map.id 
_em_map.annotation_details 
_em_map.axis_order_fast 
_em_map.axis_order_medium 
_em_map.axis_order_slow 
_em_map.cell_a 
_em_map.cell_alpha 
_em_map.cell_b 
_em_map.cell_beta 
_em_map.cell_c 
_em_map.cell_gamma 
_em_map.contour_level 
_em_map.contour_level_source 
_em_map.data_type 
_em_map.dimensions_col 
_em_map.dimensions_row 
_em_map.dimensions_sec 
_em_map.endian_type 
_em_map.file 
_em_map.format 
_em_map.label 
_em_map.limit_col 
_em_map.limit_row 
_em_map.limit_sec 
_em_map.origin_col 
_em_map.origin_row 
_em_map.origin_sec 
_em_map.partition 
_em_map.pixel_spacing_x 
_em_map.pixel_spacing_y 
_em_map.pixel_spacing_z 
_em_map.size_kb 
_em_map.spacing_x 
_em_map.spacing_y 
_em_map.spacing_z 
_em_map.statistics_average 
_em_map.statistics_maximum 
_em_map.statistics_minimum 
_em_map.statistics_std 
_em_map.symmetry_space_group 
_em_map.type 
EMD-14647 1 'RELION local-resolution filtered map' X Y Z 241.056 90.0 241.056 90.0 241.056 90.0 0.03  author 
'Image stored as floating point number (4 bytes)' 288 288 288 big emd_14647.map.gz              CCP4 
::::EMDATABANK.org::::EMD-14647:::: 287 287 287 0 0 0 1 0.837 0.837 0.837 95552512 288 288 288 2.4005536E-4 0.15768294  
-0.09757073  0.0038915705 1 'primary map'    
EMD-14647 2 'RELION 3D refinement half map 1'      X Y Z 241.056 90.0 241.056 90.0 241.056 90.0 0.016 author 
'Image stored as floating point number (4 bytes)' 288 288 288 big emd_14647_half_map_1.map.gz   CCP4 
::::EMDATABANK.org::::EMD-14647:::: 287 287 287 0 0 0 1 0.837 0.837 0.837 95552512 288 288 288 1.2290715E-4 0.050277743 
-0.025435971 0.0034198454 1 'half map'       
EMD-14647 3 'RELION 3D refinement half map 2'      X Y Z 241.056 90.0 241.056 90.0 241.056 90.0 0.016 author 
'Image stored as floating point number (4 bytes)' 288 288 288 big emd_14647_half_map_2.map.gz   CCP4 
::::EMDATABANK.org::::EMD-14647:::: 287 287 287 0 0 0 2 0.837 0.837 0.837 95552512 288 288 288 1.2194257E-4 0.047760457 
-0.027634187 0.002883403  1 'half map'       
EMD-14647 4 'RELION masked 3D refinement map'      X Y Z 241.056 90.0 241.056 90.0 241.056 90.0 0.012 author 
'Image stored as floating point number (4 bytes)' 288 288 288 big emd_14647_additional_1.map.gz CCP4 
::::EMDATABANK.org::::EMD-14647:::: 287 287 287 0 0 0 1 0.837 0.837 0.837 95552512 288 288 288 3.556988E-4  0.03530271  
-0.013721461 0.0012597078 1 'additional map' 
EMD-14647 5 'RELION masked postprocessing map'     X Y Z 241.056 90.0 241.056 90.0 241.056 90.0 0.016 author 
'Image stored as floating point number (4 bytes)' 288 288 288 big emd_14647_additional_2.map.gz CCP4 
::::EMDATABANK.org::::EMD-14647:::: 287 287 287 0 0 0 2 0.837 0.837 0.837 95552512 288 288 288 1.0975099E-4 0.08252544  
-0.049365867 0.0020195371 1 'additional map' 
# 
loop_
_em_software.id 
_em_software.category 
_em_software.details 
_em_software.name 
_em_software.version 
_em_software.image_processing_id 
_em_software.fitting_id 
_em_software.imaging_id 
1  'PARTICLE SELECTION'       ? ? ? 1 ? ? 
2  'IMAGE ACQUISITION'        ? ? ? ? ? 1 
3  MASKING                    ? ? ? ? ? ? 
4  'CTF CORRECTION'           ? ? ? 1 ? ? 
5  'LAYERLINE INDEXING'       ? ? ? ? ? ? 
6  'DIFFRACTION INDEXING'     ? ? ? ? ? ? 
7  'MODEL FITTING'            ? ? ? ? ? ? 
8  'MODEL REFINEMENT'         ? ? ? ? ? ? 
9  OTHER                      ? ? ? ? ? ? 
10 'INITIAL EULER ASSIGNMENT' ? ? ? 1 ? ? 
11 'FINAL EULER ASSIGNMENT'   ? ? ? 1 ? ? 
12 CLASSIFICATION             ? ? ? 1 ? ? 
13 RECONSTRUCTION             ? ? ? 1 ? ? 
# 
_em_specimen.id                      1 
_em_specimen.experiment_id           1 
_em_specimen.concentration           1.5 
_em_specimen.details                 ? 
_em_specimen.embedding_applied       NO 
_em_specimen.shadowing_applied       NO 
_em_specimen.staining_applied        NO 
_em_specimen.vitrification_applied   YES 
# 
_em_start_model.id                               1 
_em_start_model.details                          ? 
_em_start_model.image_processing_id              1 
_em_start_model.emdb_id                          ? 
_em_start_model.insilico_model                   ? 
_em_start_model.orthogonal_tilt_angle1           ? 
_em_start_model.orthogonal_tilt_angle2           ? 
_em_start_model.orthogonal_tilt_num_images       ? 
_em_start_model.other                            ? 
_em_start_model.pdb_id                           ? 
_em_start_model.random_conical_tilt_angle        ? 
_em_start_model.random_conical_tilt_num_images   ? 
_em_start_model.type                             NONE 
# 
_em_support_film.id                  1 
_em_support_film.sample_support_id   1 
_em_support_film.material            CARBON 
_em_support_film.thickness           ? 
_em_support_film.topology            HOLEY 
# 
_pdbx_audit_support.funding_organization   'Max Planck Society' 
_pdbx_audit_support.country                Germany 
_pdbx_audit_support.grant_number           ? 
_pdbx_audit_support.details                ? 
_pdbx_audit_support.ordinal                1 
# 
