data_EMD-13864
# 
_entry.id   EMD-13864 
# 
_audit_conform.dict_name       mmcif_pdbx.dic 
_audit_conform.dict_version    5.395 
_audit_conform.dict_location   http://mmcif.pdb.org/dictionaries/ascii/mmcif_pdbx.dic 
# 
loop_
_database_2.database_id 
_database_2.database_code 
_database_2.pdbx_database_accession 
_database_2.pdbx_DOI 
PDB   7Q8C         pdb_00007q8c 10.2210/pdb7q8c/pdb 
WWPDB D_1292119148 ?            ?                   
EMDB  EMD-13864    ?            ?                   
# 
loop_
_pdbx_database_related.db_name 
_pdbx_database_related.details 
_pdbx_database_related.db_id 
_pdbx_database_related.content_type 
PDB  .                                              7Q8B      unspecified            
EMDB .                                              EMD-13863 'other EM volume'      
EMDB 'Leishmania major actin filament in ADP-state' EMD-13864 'associated EM volume' 
# 
_citation.abstract                  ? 
_citation.abstract_id_CAS           ? 
_citation.book_id_ISBN              ? 
_citation.book_publisher            ? 
_citation.book_publisher_city       ? 
_citation.book_title                ? 
_citation.coordinate_linkage        ? 
_citation.country                   UK 
_citation.database_id_Medline       ? 
_citation.details                   ? 
_citation.id                        primary 
_citation.journal_abbrev            'Nat Commun' 
_citation.journal_id_ASTM           ? 
_citation.journal_id_CSD            ? 
_citation.journal_id_ISSN           2041-1723 
_citation.journal_full              ? 
_citation.journal_issue             ? 
_citation.journal_volume            13 
_citation.language                  ? 
_citation.page_first                3442 
_citation.page_last                 3442 
_citation.title                     'Structural basis of rapid actin dynamics in the evolutionarily divergent Leishmania parasite.' 
_citation.year                      2022 
_citation.database_id_CSD           ? 
_citation.pdbx_database_id_DOI      10.1038/s41467-022-31068-y 
_citation.pdbx_database_id_PubMed   35705539 
_citation.pdbx_database_id_patent   ? 
_citation.unpublished_flag          ? 
# 
loop_
_citation_author.citation_id 
_citation_author.name 
_citation_author.ordinal 
_citation_author.identifier_ORCID 
primary 'Kotila, T.'        1 0000-0002-9046-5834 
primary 'Wioland, H.'       2 0000-0001-5254-9642 
primary 'Selvaraj, M.'      3 0000-0001-7793-6323 
primary 'Kogan, K.'         4 0000-0003-3270-9391 
primary 'Antenucci, L.'     5 ?                   
primary 'Jegou, A.'         6 0000-0003-0356-3127 
primary 'Huiskonen, J.T.'   7 0000-0002-0348-7323 
primary 'Romet-Lemonne, G.' 8 ?                   
primary 'Lappalainen, P.'   9 0000-0001-6227-0354 
# 
loop_
_entity.id 
_entity.type 
_entity.src_method 
_entity.pdbx_description 
_entity.formula_weight 
_entity.pdbx_number_of_molecules 
_entity.pdbx_ec 
_entity.pdbx_mutation 
_entity.pdbx_fragment 
_entity.details 
1 polymer     man Actin                      42063.867 5   ? ? ? ? 
2 non-polymer syn "ADENOSINE-5'-DIPHOSPHATE" 427.201   5   ? ? ? ? 
3 non-polymer syn 'MAGNESIUM ION'            24.305    5   ? ? ? ? 
4 water       nat water                      18.015    360 ? ? ? ? 
# 
_entity_poly.entity_id                  1 
_entity_poly.type                       'polypeptide(L)' 
_entity_poly.pdbx_seq_one_letter_code   
;MADNEQSSIVCDNGSGMVKAGFSGDDAPRHVFPSIVGRPKNMQAMMGSANKTVYVGDEAQSKRGVLSLKYPIEHGIVTNW
DDMEKIWHHTFYNELRVNPEQHNVLLTEAPMNPKQNREKMTQIMFETFNVPSLYIGIQAVLSLYSSGRTTGIVLDAGDGV
THTVPIYEGYSLPHAVRRVDMAGRDLTEYLMKIMMETGTTFTTTAEKEIVRNVKEQLCYVALDFEEEMTNSAKSANEEAF
ELPDGNVMMVGNQRFRCPEVLFKPSLIGLDEAPGFPEMVYQSINKCDIDVRRELYGNIVLSGGSTMFLNLPERLAKEISN
LAPSSIKPKVVAPPERKYSVWIGGSILSSLTTFQTMWVKKSEYDESGPSIVHNKCF
;
# 
loop_
_pdbx_entity_nonpoly.entity_id 
_pdbx_entity_nonpoly.name 
_pdbx_entity_nonpoly.comp_id 
2 "ADENOSINE-5'-DIPHOSPHATE" ADP 
3 'MAGNESIUM ION'            MG  
4 water                      HOH 
# 
_entity_src_gen.entity_id                          1 
_entity_src_gen.pdbx_src_id                        1 
_entity_src_gen.pdbx_alt_source_flag               sample 
_entity_src_gen.pdbx_seq_type                      'Biological sequence' 
_entity_src_gen.pdbx_beg_seq_num                   1 
_entity_src_gen.pdbx_end_seq_num                   376 
_entity_src_gen.gene_src_common_name               ? 
_entity_src_gen.gene_src_genus                     ? 
_entity_src_gen.pdbx_gene_src_gene                 'ACT, LMJF_04_1230' 
_entity_src_gen.gene_src_species                   ? 
_entity_src_gen.gene_src_strain                    ? 
_entity_src_gen.gene_src_tissue                    ? 
_entity_src_gen.gene_src_tissue_fraction           ? 
_entity_src_gen.gene_src_details                   ? 
_entity_src_gen.pdbx_gene_src_fragment             ? 
_entity_src_gen.pdbx_gene_src_scientific_name      'Leishmania major' 
_entity_src_gen.pdbx_gene_src_ncbi_taxonomy_id     5664 
_entity_src_gen.pdbx_gene_src_variant              ? 
_entity_src_gen.pdbx_gene_src_cell_line            ? 
_entity_src_gen.pdbx_gene_src_atcc                 ? 
_entity_src_gen.pdbx_gene_src_culture_collection   ? 
_entity_src_gen.pdbx_gene_src_organ                ? 
_entity_src_gen.pdbx_gene_src_organelle            ? 
_entity_src_gen.pdbx_gene_src_cell                 ? 
_entity_src_gen.pdbx_gene_src_cellular_location    ? 
_entity_src_gen.host_org_common_name               ? 
_entity_src_gen.pdbx_host_org_scientific_name      'Spodoptera frugiperda' 
_entity_src_gen.pdbx_host_org_ncbi_taxonomy_id     7108 
_entity_src_gen.host_org_genus                     ? 
_entity_src_gen.pdbx_host_org_gene                 ? 
_entity_src_gen.pdbx_host_org_organ                ? 
_entity_src_gen.host_org_species                   ? 
_entity_src_gen.pdbx_host_org_tissue               ? 
_entity_src_gen.pdbx_host_org_tissue_fraction      ? 
_entity_src_gen.pdbx_host_org_strain               ? 
_entity_src_gen.pdbx_host_org_variant              ? 
_entity_src_gen.pdbx_host_org_cell_line            ? 
_entity_src_gen.pdbx_host_org_atcc                 ? 
_entity_src_gen.pdbx_host_org_culture_collection   ? 
_entity_src_gen.pdbx_host_org_cell                 ? 
_entity_src_gen.pdbx_host_org_organelle            ? 
_entity_src_gen.pdbx_host_org_cellular_location    ? 
_entity_src_gen.pdbx_host_org_vector_type          ? 
_entity_src_gen.pdbx_host_org_vector               ? 
_entity_src_gen.host_org_details                   ? 
_entity_src_gen.expression_system_id               ? 
_entity_src_gen.plasmid_name                       ? 
_entity_src_gen.plasmid_details                    ? 
_entity_src_gen.pdbx_description                   ? 
# 
loop_
_chem_comp.id 
_chem_comp.type 
_chem_comp.mon_nstd_flag 
_chem_comp.name 
_chem_comp.pdbx_synonyms 
_chem_comp.formula 
_chem_comp.formula_weight 
ADP non-polymer         n "ADENOSINE-5'-DIPHOSPHATE" ? 'C10 H15 N5 O10 P2' 427.201 
ALA 'L-peptide linking' y ALANINE                    ? 'C3 H7 N O2'        89.093  
ARG 'L-peptide linking' y ARGININE                   ? 'C6 H15 N4 O2 1'    175.209 
ASN 'L-peptide linking' y ASPARAGINE                 ? 'C4 H8 N2 O3'       132.118 
ASP 'L-peptide linking' y 'ASPARTIC ACID'            ? 'C4 H7 N O4'        133.103 
CYS 'L-peptide linking' y CYSTEINE                   ? 'C3 H7 N O2 S'      121.158 
GLN 'L-peptide linking' y GLUTAMINE                  ? 'C5 H10 N2 O3'      146.144 
GLU 'L-peptide linking' y 'GLUTAMIC ACID'            ? 'C5 H9 N O4'        147.129 
GLY 'peptide linking'   y GLYCINE                    ? 'C2 H5 N O2'        75.067  
HIS 'L-peptide linking' y HISTIDINE                  ? 'C6 H10 N3 O2 1'    156.162 
HOH non-polymer         . WATER                      ? 'H2 O'              18.015  
ILE 'L-peptide linking' y ISOLEUCINE                 ? 'C6 H13 N O2'       131.173 
LEU 'L-peptide linking' y LEUCINE                    ? 'C6 H13 N O2'       131.173 
LYS 'L-peptide linking' y LYSINE                     ? 'C6 H15 N2 O2 1'    147.195 
MET 'L-peptide linking' y METHIONINE                 ? 'C5 H11 N O2 S'     149.211 
MG  non-polymer         . 'MAGNESIUM ION'            ? 'Mg 2'              24.305  
PHE 'L-peptide linking' y PHENYLALANINE              ? 'C9 H11 N O2'       165.189 
PRO 'L-peptide linking' y PROLINE                    ? 'C5 H9 N O2'        115.130 
SER 'L-peptide linking' y SERINE                     ? 'C3 H7 N O3'        105.093 
THR 'L-peptide linking' y THREONINE                  ? 'C4 H9 N O3'        119.119 
TRP 'L-peptide linking' y TRYPTOPHAN                 ? 'C11 H12 N2 O2'     204.225 
TYR 'L-peptide linking' y TYROSINE                   ? 'C9 H11 N O3'       181.189 
VAL 'L-peptide linking' y VALINE                     ? 'C5 H11 N O2'       117.146 
# 
_exptl.entry_id         EMD-13864 
_exptl.details          ? 
_exptl.method           'ELECTRON MICROSCOPY' 
_exptl.method_details   ? 
# 
_struct_keywords.entry_id        EMD-13864 
_struct_keywords.text            'Actin, Filament, Parasite, ADP-Pi, STRUCTURAL PROTEIN' 
_struct_keywords.pdbx_keywords   'STRUCTURAL PROTEIN' 
_struct_keywords.pdbx_details    ? 
# 
_struct_ref.id          1 
_struct_ref.db_name     UNP 
_struct_ref.db_code     Q9U1E8_LEIMA 
_struct_ref.entity_id   1 
# 
_em_3d_fitting.entry_id          EMD-13864 
_em_3d_fitting.id                1 
_em_3d_fitting.details           ? 
_em_3d_fitting.overall_b_value   ? 
_em_3d_fitting.ref_protocol      ? 
_em_3d_fitting.ref_space         REAL 
_em_3d_fitting.target_criteria   ? 
_em_3d_fitting.method            ? 
# 
_em_3d_fitting_list.3d_fitting_id                 1 
_em_3d_fitting_list.id                            1 
_em_3d_fitting_list.details                       ? 
_em_3d_fitting_list.pdb_chain_id                  A 
_em_3d_fitting_list.pdb_chain_residue_range       ? 
_em_3d_fitting_list.pdb_entry_id                  6DJO 
_em_3d_fitting_list.initial_refinement_model_id   1 
_em_3d_fitting_list.chain_id                      ? 
_em_3d_fitting_list.chain_residue_range           ? 
_em_3d_fitting_list.source_name                   PDB 
_em_3d_fitting_list.type                          'experimental model' 
_em_3d_fitting_list.accession_code                6DJO 
# 
_em_3d_reconstruction.entry_id                    EMD-13864 
_em_3d_reconstruction.id                          1 
_em_3d_reconstruction.algorithm                   'FOURIER SPACE' 
_em_3d_reconstruction.details                     ? 
_em_3d_reconstruction.fsc_type                    'EVEN/ODD MAPS REFINED TOTALLY INDEPENDENT (GOLD STANDARD)' 
_em_3d_reconstruction.refinement_type             ? 
_em_3d_reconstruction.image_processing_id         1 
_em_3d_reconstruction.num_class_averages          ? 
_em_3d_reconstruction.num_particles               232527 
_em_3d_reconstruction.resolution                  2.72 
_em_3d_reconstruction.resolution_method           'FSC 0.143 CUT-OFF' 
_em_3d_reconstruction.symmetry_type               HELICAL 
_em_3d_reconstruction.method                      ? 
_em_3d_reconstruction.nominal_pixel_size          ? 
_em_3d_reconstruction.actual_pixel_size           ? 
_em_3d_reconstruction.magnification_calibration   ? 
# 
_em_buffer.id            1 
_em_buffer.details       '10 mM HEPES, 125 mM NaCl, 5 mM KCl, 0.2 mM ATP, 0.4 mM EGTA, 1 mM MgCl2, 1  mM DTT' 
_em_buffer.pH            7.4 
_em_buffer.specimen_id   1 
_em_buffer.name          ? 
# 
_em_entity_assembly.id                   1 
_em_entity_assembly.parent_id            0 
_em_entity_assembly.details              ? 
_em_entity_assembly.go_id                ? 
_em_entity_assembly.name                 
'Bare, undecorated, ADP-state actin filament from the sample mixed with Leishmania major cofilin.' 
_em_entity_assembly.source               RECOMBINANT 
_em_entity_assembly.type                 COMPLEX 
_em_entity_assembly.chimera              ? 
_em_entity_assembly.entity_id_list       1 
_em_entity_assembly.ipr_id               ? 
_em_entity_assembly.synonym              ? 
_em_entity_assembly.number_of_copies     ? 
_em_entity_assembly.oligomeric_details   ? 
# 
_em_imaging.id                              1 
_em_imaging.entry_id                        EMD-13864 
_em_imaging.accelerating_voltage            300 
_em_imaging.alignment_procedure             ? 
_em_imaging.c2_aperture_diameter            ? 
_em_imaging.calibrated_defocus_max          ? 
_em_imaging.calibrated_defocus_min          ? 
_em_imaging.calibrated_magnification        ? 
_em_imaging.cryogen                         ? 
_em_imaging.details                         ? 
_em_imaging.electron_source                 'FIELD EMISSION GUN' 
_em_imaging.illumination_mode               'FLOOD BEAM' 
_em_imaging.microscope_model                'FEI TITAN KRIOS' 
_em_imaging.mode                            'BRIGHT FIELD' 
_em_imaging.nominal_cs                      ? 
_em_imaging.nominal_defocus_max             ? 
_em_imaging.nominal_defocus_min             ? 
_em_imaging.nominal_magnification           ? 
_em_imaging.recording_temperature_maximum   ? 
_em_imaging.recording_temperature_minimum   ? 
_em_imaging.residual_tilt                   ? 
_em_imaging.specimen_holder_model           ? 
_em_imaging.specimen_id                     1 
_em_imaging.sample_support_id               1 
_em_imaging.citation_id                     ? 
_em_imaging.detector_id                     1 
_em_imaging.scans_id                        1 
_em_imaging.date                            ? 
_em_imaging.temperature                     ? 
_em_imaging.tilt_angle_min                  ? 
_em_imaging.tilt_angle_max                  ? 
_em_imaging.astigmatism                     ? 
_em_imaging.detector_distance               ? 
_em_imaging.electron_beam_tilt_params       ? 
_em_imaging.specimen_holder_type            ? 
# 
_em_sample_support.id               1 
_em_sample_support.specimen_id      1 
_em_sample_support.details          ? 
_em_sample_support.grid_material    COPPER 
_em_sample_support.grid_mesh_size   300 
_em_sample_support.grid_type        'Quantifoil R1.2/1.3' 
_em_sample_support.method           ? 
_em_sample_support.film_material    ? 
# 
_em_vitrification.id                      1 
_em_vitrification.specimen_id             1 
_em_vitrification.chamber_temperature     279.15 
_em_vitrification.cryogen_name            ETHANE 
_em_vitrification.details                 'blot for 5 seconds before plunging, blot force 15' 
_em_vitrification.humidity                95 
_em_vitrification.instrument              'FEI VITROBOT MARK IV' 
_em_vitrification.entry_id                EMD-13864 
_em_vitrification.sample_preparation_id   ? 
_em_vitrification.citation_id             ? 
_em_vitrification.method                  ? 
_em_vitrification.temp                    ? 
_em_vitrification.time_resolved_state     ? 
# 
_em_experiment.entry_id                EMD-13864 
_em_experiment.id                      1 
_em_experiment.aggregation_state       FILAMENT 
_em_experiment.reconstruction_method   HELICAL 
_em_experiment.entity_assembly_id      1 
# 
_em_admin.entry_id              EMD-13864 
_em_admin.current_status        REL 
_em_admin.deposition_date       2021-11-11 
_em_admin.deposition_site       PDBE 
_em_admin.details               ? 
_em_admin.last_update           2024-07-17 
_em_admin.map_release_date      2022-05-18 
_em_admin.obsoleted_date        ? 
_em_admin.title                 'Leishmania major actin filament in ADP-state' 
_em_admin.header_release_date   2022-05-18 
# 
loop_
_em_author_list.ordinal 
_em_author_list.author 
_em_author_list.identifier_ORCID 
1 'Kotila, T.'      ? 
2 'Muniyandi, S.'   ? 
3 'Lappalainen, P.' ? 
4 'Huiskonen, J.T.' ? 
# 
_em_ctf_correction.id                                1 
_em_ctf_correction.em_image_processing_id            1 
_em_ctf_correction.type                              'PHASE FLIPPING AND AMPLITUDE CORRECTION' 
_em_ctf_correction.details                           ? 
_em_ctf_correction.amplitude_correction              ? 
_em_ctf_correction.amplitude_correction_factor       ? 
_em_ctf_correction.amplitude_correction_space        ? 
_em_ctf_correction.correction_operation              ? 
_em_ctf_correction.phase_reversal                    ? 
_em_ctf_correction.phase_reversal_anisotropic        ? 
_em_ctf_correction.phase_reversal_correction_space   ? 
# 
_em_db_reference.id             1 
_em_db_reference.access_code    7Q8C 
_em_db_reference.db_name        PDB 
_em_db_reference.details        ? 
_em_db_reference.relationship   'IN FRAME' 
# 
_em_entity_assembly_molwt.entity_assembly_id   1 
_em_entity_assembly_molwt.id                   1 
_em_entity_assembly_molwt.experimental_flag    NO 
_em_entity_assembly_molwt.units                KILODALTONS/NANOMETER 
_em_entity_assembly_molwt.value                ? 
# 
_em_entity_assembly_naturalsource.id                   2 
_em_entity_assembly_naturalsource.entity_assembly_id   1 
_em_entity_assembly_naturalsource.cell                 ? 
_em_entity_assembly_naturalsource.cellular_location    ? 
_em_entity_assembly_naturalsource.ncbi_tax_id          5664 
_em_entity_assembly_naturalsource.organ                ? 
_em_entity_assembly_naturalsource.organelle            ? 
_em_entity_assembly_naturalsource.organism             'Leishmania major' 
_em_entity_assembly_naturalsource.strain               ? 
_em_entity_assembly_naturalsource.tissue               ? 
# 
_em_entity_assembly_recombinant.id                   2 
_em_entity_assembly_recombinant.entity_assembly_id   1 
_em_entity_assembly_recombinant.cell                 ? 
_em_entity_assembly_recombinant.ncbi_tax_id          7108 
_em_entity_assembly_recombinant.organism             'Spodoptera frugiperda' 
_em_entity_assembly_recombinant.plasmid              ? 
_em_entity_assembly_recombinant.strain               ? 
# 
loop_
_em_euler_angle_assignment.id 
_em_euler_angle_assignment.details 
_em_euler_angle_assignment.image_processing_id 
_em_euler_angle_assignment.order 
_em_euler_angle_assignment.proj_matching_angular_sampling 
_em_euler_angle_assignment.proj_matching_merit_function 
_em_euler_angle_assignment.proj_matching_num_projections 
_em_euler_angle_assignment.type 
1 ? 1 INITIAL ? ? ? 'NOT APPLICABLE' 
2 ? 1 FINAL   ? ? ? 'NOT APPLICABLE' 
# 
_em_final_classification.id                         1 
_em_final_classification.type                       3D 
_em_final_classification.avg_num_images_per_class   ? 
_em_final_classification.details                    ? 
_em_final_classification.image_processing_id        1 
_em_final_classification.num_classes                ? 
# 
_em_grid_pretreatment.id                  1 
_em_grid_pretreatment.sample_support_id   1 
_em_grid_pretreatment.atmosphere          ? 
_em_grid_pretreatment.pressure            ? 
_em_grid_pretreatment.time                45 
_em_grid_pretreatment.type                'GLOW DISCHARGE' 
# 
_em_helical_entity.id                             1 
_em_helical_entity.image_processing_id            1 
_em_helical_entity.angular_rotation_per_subunit   -166.54 
_em_helical_entity.axial_rise_per_subunit         28.4 
_em_helical_entity.axial_symmetry                 C1 
_em_helical_entity.details                        ? 
_em_helical_entity.entity_assembly_id             1 
# 
_em_image_processing.id                   1 
_em_image_processing.image_recording_id   1 
_em_image_processing.details              ? 
# 
_em_image_recording.id                                  1 
_em_image_recording.imaging_id                          1 
_em_image_recording.avg_electron_dose_per_image         55 
_em_image_recording.average_exposure_time               ? 
_em_image_recording.details                             ? 
_em_image_recording.detector_mode                       ? 
_em_image_recording.film_or_detector_model              'GATAN K3 BIOQUANTUM (6k x 4k)' 
_em_image_recording.num_diffraction_images              ? 
_em_image_recording.num_grids_imaged                    ? 
_em_image_recording.num_real_images                     50 
_em_image_recording.avg_electron_dose_per_subtomogram   ? 
# 
loop_
_em_map.entry_id 
_em_map.id 
_em_map.annotation_details 
_em_map.axis_order_fast 
_em_map.axis_order_medium 
_em_map.axis_order_slow 
_em_map.cell_a 
_em_map.cell_alpha 
_em_map.cell_b 
_em_map.cell_beta 
_em_map.cell_c 
_em_map.cell_gamma 
_em_map.contour_level 
_em_map.contour_level_source 
_em_map.data_type 
_em_map.dimensions_col 
_em_map.dimensions_row 
_em_map.dimensions_sec 
_em_map.endian_type 
_em_map.file 
_em_map.format 
_em_map.label 
_em_map.limit_col 
_em_map.limit_row 
_em_map.limit_sec 
_em_map.origin_col 
_em_map.origin_row 
_em_map.origin_sec 
_em_map.partition 
_em_map.pixel_spacing_x 
_em_map.pixel_spacing_y 
_em_map.pixel_spacing_z 
_em_map.size_kb 
_em_map.spacing_x 
_em_map.spacing_y 
_em_map.spacing_z 
_em_map.statistics_average 
_em_map.statistics_maximum 
_em_map.statistics_minimum 
_em_map.statistics_std 
_em_map.symmetry_space_group 
_em_map.type 
EMD-13864 1 'Autosharpened and real-space symmetrized map from postprocess' Z Y X 248.1696 90.0 248.1696 90.0 248.1696 90.0 4     
author 'Image stored as floating point number (4 bytes)' 288 288 288 big emd_13864.map.gz              CCP4 
::::EMDATABANK.org::::EMD-13864:::: 287 287 287 0 0 0 1 0.8617 0.8617 0.8617 95552512 288 288 288 -2.8342565E-12 34.764954   
-11.464039   1.0          1 'primary map'    
EMD-13864 2 ?                                                               X Y Z 248.1696 90.0 248.1696 90.0 248.1696 90.0 0.02  
author 'Image stored as floating point number (4 bytes)' 288 288 288 big emd_13864_additional_1.map.gz CCP4 
::::EMDATABANK.org::::EMD-13864:::: 287 287 287 0 0 0 1 0.8617 0.8617 0.8617 95552512 288 288 288 -1.0177687E-4  0.15204321  
-0.06359878  0.005580609  1 'additional map' 
EMD-13864 3 'Mask for postprocess'                                          X Y Z 248.1696 90.0 248.1696 90.0 248.1696 90.0 0.5   
author 'Image stored as floating point number (4 bytes)' 288 288 288 big emd_13864_msk_1.map           CCP4 
::::EMDATABANK.org::::EMD-13864:::: 287 287 287 0 0 0 1 0.8617 0.8617 0.8617 95552512 288 288 288 0.040192902    1.0         0.0 
0.1870011    1 mask             
EMD-13864 4 Half-map1                                                       X Y Z 248.1696 90.0 248.1696 90.0 248.1696 90.0 0.012 
author 'Image stored as floating point number (4 bytes)' 288 288 288 big emd_13864_half_map_2.map.gz   CCP4 
::::EMDATABANK.org::::EMD-13864:::: 287 287 287 0 0 0 2 0.8617 0.8617 0.8617 95552512 288 288 288 -1.0566153E-4  0.061328024 
-0.018803706 0.0026907492 1 'half map'       
EMD-13864 5 Half-map2                                                       X Y Z 248.1696 90.0 248.1696 90.0 248.1696 90.0 0.012 
author 'Image stored as floating point number (4 bytes)' 288 288 288 big emd_13864_half_map_1.map.gz   CCP4 
::::EMDATABANK.org::::EMD-13864:::: 287 287 287 0 0 0 1 0.8617 0.8617 0.8617 95552512 288 288 288 -9.789222E-5   0.056886867 
-0.018567938 0.0027066313 1 'half map'       
# 
loop_
_em_software.id 
_em_software.category 
_em_software.details 
_em_software.name 
_em_software.version 
_em_software.image_processing_id 
_em_software.fitting_id 
_em_software.imaging_id 
1  'PARTICLE SELECTION'       ?             RELION          3.1     1 ? ? 
2  'IMAGE ACQUISITION'        ?             ?               ?       ? ? 1 
3  MASKING                    ?             ?               ?       ? ? ? 
4  'CTF CORRECTION'           ?             CTFFIND         4.1     1 ? ? 
5  'LAYERLINE INDEXING'       ?             ?               ?       ? ? ? 
6  'DIFFRACTION INDEXING'     ?             ?               ?       ? ? ? 
7  'MODEL FITTING'            'build 42094' 'UCSF Chimera'  1.14    ? 1 ? 
8  'MODEL FITTING'            ?             Coot            0.8.9.1 ? 1 ? 
9  OTHER                      ?             ?               ?       ? ? ? 
10 'INITIAL EULER ASSIGNMENT' ?             RELION          3.1     1 ? ? 
11 'FINAL EULER ASSIGNMENT'   ?             RELION          3.1     1 ? ? 
12 CLASSIFICATION             ?             RELION          3.1     1 ? ? 
13 RECONSTRUCTION             ?             RELION          3.1     1 ? ? 
14 'MODEL REFINEMENT'         ?             'UCSF ChimeraX' 1.1.1   ? 1 ? 
15 'MODEL REFINEMENT'         ?             ISOLDE          1.1.0   ? 1 ? 
16 'MODEL REFINEMENT'         ?             PHENIX          1.19.2  ? 1 ? 
# 
_em_specimen.id                      1 
_em_specimen.experiment_id           1 
_em_specimen.concentration           0.5 
_em_specimen.details                 ? 
_em_specimen.embedding_applied       NO 
_em_specimen.shadowing_applied       NO 
_em_specimen.staining_applied        NO 
_em_specimen.vitrification_applied   YES 
# 
_em_start_model.id                               1 
_em_start_model.details                          ? 
_em_start_model.image_processing_id              1 
_em_start_model.emdb_id                          ? 
_em_start_model.insilico_model                   ? 
_em_start_model.orthogonal_tilt_angle1           ? 
_em_start_model.orthogonal_tilt_angle2           ? 
_em_start_model.orthogonal_tilt_num_images       ? 
_em_start_model.other                            ? 
_em_start_model.pdb_id                           ? 
_em_start_model.random_conical_tilt_angle        ? 
_em_start_model.random_conical_tilt_num_images   ? 
_em_start_model.type                             OTHER 
# 
_em_support_film.id                  1 
_em_support_film.sample_support_id   1 
_em_support_film.material            CARBON 
_em_support_film.thickness           ? 
_em_support_film.topology            HOLEY 
# 
loop_
_pdbx_audit_support.funding_organization 
_pdbx_audit_support.country 
_pdbx_audit_support.grant_number 
_pdbx_audit_support.details 
_pdbx_audit_support.ordinal 
'Academy of Finland'              Finland 320161  ? 1 
'Jane and Aatos Erkko Foundation' Finland 4708679 ? 2 
# 
_pdbx_initial_refinement_model.id               1 
_pdbx_initial_refinement_model.type             'experimental model' 
_pdbx_initial_refinement_model.source_name      PDB 
_pdbx_initial_refinement_model.accession_code   6DJO 
# 
