data_EMD-13008
# 
_entry.id   EMD-13008 
# 
_audit_conform.dict_name       mmcif_pdbx.dic 
_audit_conform.dict_version    5.395 
_audit_conform.dict_location   http://mmcif.pdb.org/dictionaries/ascii/mmcif_pdbx.dic 
# 
loop_
_database_2.database_id 
_database_2.database_code 
_database_2.pdbx_database_accession 
_database_2.pdbx_DOI 
PDB   7OOA         pdb_00007ooa 10.2210/pdb7ooa/pdb 
WWPDB D_1292116130 ?            ?                   
EMDB  EMD-13008    ?            ?                   
# 
loop_
_pdbx_database_related.db_name 
_pdbx_database_related.details 
_pdbx_database_related.db_id 
_pdbx_database_related.content_type 
EMDB .                                                                                           EMD-12997 'other EM volume'      
EMDB .                                                                                           EMD-13003 'other EM volume'      
EMDB .                                                                                           EMD-13006 'other EM volume'      
EMDB .                                                                                           EMD-12996 'other EM volume'      
EMDB .                                                                                           EMD-13007 'other EM volume'      
EMDB 'Mechanosensitive channel MscS solubilized with LMNG in open conformation with added lipid' EMD-13008 'associated EM volume' 
# 
_citation.abstract                  ? 
_citation.abstract_id_CAS           ? 
_citation.book_id_ISBN              ? 
_citation.book_publisher            ? 
_citation.book_publisher_city       ? 
_citation.book_title                ? 
_citation.coordinate_linkage        ? 
_citation.country                   US 
_citation.database_id_Medline       ? 
_citation.details                   ? 
_citation.id                        primary 
_citation.journal_abbrev            Proc.Natl.Acad.Sci.USA 
_citation.journal_id_ASTM           PNASA6 
_citation.journal_id_CSD            0040 
_citation.journal_id_ISSN           1091-6490 
_citation.journal_full              ? 
_citation.journal_issue             ? 
_citation.journal_volume            118 
_citation.language                  ? 
_citation.page_first                ? 
_citation.page_last                 ? 
_citation.title                     'Mechanosensitive channel gating by delipidation.' 
_citation.year                      2021 
_citation.database_id_CSD           ? 
_citation.pdbx_database_id_DOI      10.1073/pnas.2107095118 
_citation.pdbx_database_id_PubMed   34376558 
_citation.pdbx_database_id_patent   ? 
_citation.unpublished_flag          ? 
# 
loop_
_citation_author.citation_id 
_citation_author.name 
_citation_author.ordinal 
_citation_author.identifier_ORCID 
primary 'Flegler, V.J.'  1  0000-0003-3627-0603 
primary 'Rasmussen, A.'  2  0000-0001-7796-1668 
primary 'Borbil, K.'     3  ?                   
primary 'Boten, L.'      4  0000-0001-6089-4067 
primary 'Chen, H.A.'     5  ?                   
primary 'Deinlein, H.'   6  ?                   
primary 'Halang, J.'     7  ?                   
primary 'Hellmanzik, K.' 8  0000-0002-1734-8260 
primary 'Loffler, J.'    9  ?                   
primary 'Schmidt, V.'    10 ?                   
primary 'Makbul, C.'     11 ?                   
primary 'Kraft, C.'      12 ?                   
primary 'Hedrich, R.'    13 0000-0003-3224-1362 
primary 'Rasmussen, T.'  14 0000-0003-1126-846X 
primary 'Bottcher, B.'   15 0000-0002-7962-4849 
# 
loop_
_entity.id 
_entity.type 
_entity.src_method 
_entity.pdbx_description 
_entity.formula_weight 
_entity.pdbx_number_of_molecules 
_entity.pdbx_ec 
_entity.pdbx_mutation 
_entity.pdbx_fragment 
_entity.details 
1 polymer     man 'Mechanosensitive channel of small conductance (MscS)' 31994.039 7  ? ? ? ? 
2 non-polymer syn 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine          744.034   7  ? ? ? ? 
3 non-polymer syn 'Lauryl Maltose Neopentyl Glycol'                      1005.188  21 ? ? ? ? 
4 non-polymer syn DODECYL-BETA-D-MALTOSIDE                               510.615   7  ? ? ? ? 
# 
_entity_poly.entity_id                  1 
_entity_poly.type                       'polypeptide(L)' 
_entity_poly.pdbx_seq_one_letter_code   
;MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLIIARMISNAVNRLMISRKIDATVADFLSALVRYGIIAF
TLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKDREMTVRLNELGASSINFVVRVW
SNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVNFKRVKEDKAALEHHHHHH
;
# 
loop_
_pdbx_entity_nonpoly.entity_id 
_pdbx_entity_nonpoly.name 
_pdbx_entity_nonpoly.comp_id 
2 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine PEE 
3 'Lauryl Maltose Neopentyl Glycol'             AV0 
4 DODECYL-BETA-D-MALTOSIDE                      LMT 
# 
_entity_src_gen.entity_id                          1 
_entity_src_gen.pdbx_src_id                        1 
_entity_src_gen.pdbx_alt_source_flag               sample 
_entity_src_gen.pdbx_seq_type                      'Biological sequence' 
_entity_src_gen.pdbx_beg_seq_num                   1 
_entity_src_gen.pdbx_end_seq_num                   294 
_entity_src_gen.gene_src_common_name               ? 
_entity_src_gen.gene_src_genus                     ? 
_entity_src_gen.pdbx_gene_src_gene                 ? 
_entity_src_gen.gene_src_species                   ? 
_entity_src_gen.gene_src_strain                    ? 
_entity_src_gen.gene_src_tissue                    ? 
_entity_src_gen.gene_src_tissue_fraction           ? 
_entity_src_gen.gene_src_details                   ? 
_entity_src_gen.pdbx_gene_src_fragment             ? 
_entity_src_gen.pdbx_gene_src_scientific_name      ' Escherichia coli SE11' 
_entity_src_gen.pdbx_gene_src_ncbi_taxonomy_id     409438 
_entity_src_gen.pdbx_gene_src_variant              ? 
_entity_src_gen.pdbx_gene_src_cell_line            ? 
_entity_src_gen.pdbx_gene_src_atcc                 ? 
_entity_src_gen.pdbx_gene_src_culture_collection   ? 
_entity_src_gen.pdbx_gene_src_organ                ? 
_entity_src_gen.pdbx_gene_src_organelle            ? 
_entity_src_gen.pdbx_gene_src_cell                 ? 
_entity_src_gen.pdbx_gene_src_cellular_location    ? 
_entity_src_gen.host_org_common_name               ? 
_entity_src_gen.pdbx_host_org_scientific_name      'Escherichia coli' 
_entity_src_gen.pdbx_host_org_ncbi_taxonomy_id     562 
_entity_src_gen.host_org_genus                     ? 
_entity_src_gen.pdbx_host_org_gene                 ? 
_entity_src_gen.pdbx_host_org_organ                ? 
_entity_src_gen.host_org_species                   ? 
_entity_src_gen.pdbx_host_org_tissue               ? 
_entity_src_gen.pdbx_host_org_tissue_fraction      ? 
_entity_src_gen.pdbx_host_org_strain               ? 
_entity_src_gen.pdbx_host_org_variant              ? 
_entity_src_gen.pdbx_host_org_cell_line            ? 
_entity_src_gen.pdbx_host_org_atcc                 ? 
_entity_src_gen.pdbx_host_org_culture_collection   ? 
_entity_src_gen.pdbx_host_org_cell                 ? 
_entity_src_gen.pdbx_host_org_organelle            ? 
_entity_src_gen.pdbx_host_org_cellular_location    ? 
_entity_src_gen.pdbx_host_org_vector_type          ? 
_entity_src_gen.pdbx_host_org_vector               ? 
_entity_src_gen.host_org_details                   ? 
_entity_src_gen.expression_system_id               ? 
_entity_src_gen.plasmid_name                       ? 
_entity_src_gen.plasmid_details                    ? 
_entity_src_gen.pdbx_description                   ? 
# 
loop_
_chem_comp.id 
_chem_comp.type 
_chem_comp.mon_nstd_flag 
_chem_comp.name 
_chem_comp.pdbx_synonyms 
_chem_comp.formula 
_chem_comp.formula_weight 
ALA 'L-peptide linking' y ALANINE                                       ? 'C3 H7 N O2'     89.093   
ARG 'L-peptide linking' y ARGININE                                      ? 'C6 H15 N4 O2 1' 175.209  
ASN 'L-peptide linking' y ASPARAGINE                                    ? 'C4 H8 N2 O3'    132.118  
ASP 'L-peptide linking' y 'ASPARTIC ACID'                               ? 'C4 H7 N O4'     133.103  
AV0 non-polymer         . 'Lauryl Maltose Neopentyl Glycol'             
;2,2-didecylpropane-1,3-bis-b-D-maltopyranoside; 2-decyl-2-{[(4-O-alpha-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]methyl}dodecyl4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside
;
'C47 H88 O22'    1005.188 
GLN 'L-peptide linking' y GLUTAMINE                                     ? 'C5 H10 N2 O3'   146.144  
GLU 'L-peptide linking' y 'GLUTAMIC ACID'                               ? 'C5 H9 N O4'     147.129  
GLY 'peptide linking'   y GLYCINE                                       ? 'C2 H5 N O2'     75.067   
HIS 'L-peptide linking' y HISTIDINE                                     ? 'C6 H10 N3 O2 1' 156.162  
ILE 'L-peptide linking' y ISOLEUCINE                                    ? 'C6 H13 N O2'    131.173  
LEU 'L-peptide linking' y LEUCINE                                       ? 'C6 H13 N O2'    131.173  
LMT D-saccharide        . DODECYL-BETA-D-MALTOSIDE                      ? 'C24 H46 O11'    510.615  
LYS 'L-peptide linking' y LYSINE                                        ? 'C6 H15 N2 O2 1' 147.195  
MET 'L-peptide linking' y METHIONINE                                    ? 'C5 H11 N O2 S'  149.211  
PEE non-polymer         n 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine DOPE 'C41 H78 N O8 P' 744.034  
PHE 'L-peptide linking' y PHENYLALANINE                                 ? 'C9 H11 N O2'    165.189  
PRO 'L-peptide linking' y PROLINE                                       ? 'C5 H9 N O2'     115.130  
SER 'L-peptide linking' y SERINE                                        ? 'C3 H7 N O3'     105.093  
THR 'L-peptide linking' y THREONINE                                     ? 'C4 H9 N O3'     119.119  
TRP 'L-peptide linking' y TRYPTOPHAN                                    ? 'C11 H12 N2 O2'  204.225  
TYR 'L-peptide linking' y TYROSINE                                      ? 'C9 H11 N O3'    181.189  
VAL 'L-peptide linking' y VALINE                                        ? 'C5 H11 N O2'    117.146  
# 
_exptl.entry_id         EMD-13008 
_exptl.details          ? 
_exptl.method           'ELECTRON MICROSCOPY' 
_exptl.method_details   ? 
# 
_struct_keywords.entry_id        EMD-13008 
_struct_keywords.text            'mechanosensitive channel, LMNG, delipidation, MEMBRANE PROTEIN' 
_struct_keywords.pdbx_keywords   'MEMBRANE PROTEIN' 
_struct_keywords.pdbx_details    ? 
# 
_struct_ref.id          1 
_struct_ref.db_name     UNP 
_struct_ref.db_code     B6I756_ECOSE 
_struct_ref.entity_id   1 
# 
_em_3d_fitting.entry_id          EMD-13008 
_em_3d_fitting.id                1 
_em_3d_fitting.details           ? 
_em_3d_fitting.overall_b_value   ? 
_em_3d_fitting.ref_protocol      'FLEXIBLE FIT' 
_em_3d_fitting.ref_space         REAL 
_em_3d_fitting.target_criteria   'WEIGHTED MAP SUM AT ATOM CENTERS' 
_em_3d_fitting.method            ? 
# 
_em_3d_fitting_list.3d_fitting_id                 1 
_em_3d_fitting_list.id                            1 
_em_3d_fitting_list.details                       ? 
_em_3d_fitting_list.pdb_chain_id                  ? 
_em_3d_fitting_list.pdb_chain_residue_range       ? 
_em_3d_fitting_list.pdb_entry_id                  6RLD 
_em_3d_fitting_list.initial_refinement_model_id   1 
_em_3d_fitting_list.chain_id                      ? 
_em_3d_fitting_list.chain_residue_range           ? 
_em_3d_fitting_list.source_name                   PDB 
_em_3d_fitting_list.type                          'experimental model' 
_em_3d_fitting_list.accession_code                6RLD 
# 
_em_3d_reconstruction.entry_id                    EMD-13008 
_em_3d_reconstruction.id                          1 
_em_3d_reconstruction.algorithm                   'FOURIER SPACE' 
_em_3d_reconstruction.details                     ? 
_em_3d_reconstruction.fsc_type                    'EVEN/ODD MAPS REFINED TOTALLY INDEPENDENT (GOLD STANDARD)' 
_em_3d_reconstruction.refinement_type             ? 
_em_3d_reconstruction.image_processing_id         1 
_em_3d_reconstruction.num_class_averages          ? 
_em_3d_reconstruction.num_particles               262797 
_em_3d_reconstruction.resolution                  2.7 
_em_3d_reconstruction.resolution_method           'FSC 0.143 CUT-OFF' 
_em_3d_reconstruction.symmetry_type               POINT 
_em_3d_reconstruction.method                      ? 
_em_3d_reconstruction.nominal_pixel_size          ? 
_em_3d_reconstruction.actual_pixel_size           ? 
_em_3d_reconstruction.magnification_calibration   ? 
# 
_em_buffer.id            1 
_em_buffer.details       ? 
_em_buffer.pH            7.5 
_em_buffer.specimen_id   1 
_em_buffer.name          ? 
# 
_em_entity_assembly.id                   1 
_em_entity_assembly.parent_id            0 
_em_entity_assembly.details              ? 
_em_entity_assembly.go_id                ? 
_em_entity_assembly.name                 'homoheptameric complex of MscS coordinated with lipids and detergents DDM and LMNG' 
_em_entity_assembly.source               RECOMBINANT 
_em_entity_assembly.type                 COMPLEX 
_em_entity_assembly.entity_id_list       1 
_em_entity_assembly.ipr_id               ? 
_em_entity_assembly.synonym              ? 
_em_entity_assembly.number_of_copies     ? 
_em_entity_assembly.oligomeric_details   ? 
# 
_em_imaging.id                              1 
_em_imaging.entry_id                        EMD-13008 
_em_imaging.accelerating_voltage            300 
_em_imaging.alignment_procedure             'COMA FREE' 
_em_imaging.c2_aperture_diameter            70 
_em_imaging.calibrated_defocus_max          ? 
_em_imaging.calibrated_defocus_min          ? 
_em_imaging.calibrated_magnification        ? 
_em_imaging.cryogen                         NITROGEN 
_em_imaging.details                         ? 
_em_imaging.electron_source                 'FIELD EMISSION GUN' 
_em_imaging.illumination_mode               'FLOOD BEAM' 
_em_imaging.microscope_model                'FEI TITAN KRIOS' 
_em_imaging.mode                            'BRIGHT FIELD' 
_em_imaging.nominal_cs                      2.7 
_em_imaging.nominal_defocus_max             1800 
_em_imaging.nominal_defocus_min             1000 
_em_imaging.nominal_magnification           75000 
_em_imaging.recording_temperature_maximum   ? 
_em_imaging.recording_temperature_minimum   ? 
_em_imaging.residual_tilt                   ? 
_em_imaging.specimen_holder_model           'FEI TITAN KRIOS AUTOGRID HOLDER' 
_em_imaging.specimen_id                     1 
_em_imaging.sample_support_id               1 
_em_imaging.citation_id                     ? 
_em_imaging.detector_id                     1 
_em_imaging.scans_id                        1 
_em_imaging.date                            ? 
_em_imaging.temperature                     ? 
_em_imaging.tilt_angle_min                  ? 
_em_imaging.tilt_angle_max                  ? 
_em_imaging.astigmatism                     ? 
_em_imaging.detector_distance               ? 
_em_imaging.electron_beam_tilt_params       ? 
_em_imaging.specimen_holder_type            ? 
# 
_em_sample_support.id               1 
_em_sample_support.specimen_id      1 
_em_sample_support.details          ? 
_em_sample_support.grid_material    COPPER 
_em_sample_support.grid_mesh_size   400 
_em_sample_support.grid_type        'Quantifoil R1.2/1.3' 
_em_sample_support.method           ? 
_em_sample_support.film_material    ? 
# 
_em_vitrification.id                      1 
_em_vitrification.specimen_id             1 
_em_vitrification.chamber_temperature     277 
_em_vitrification.cryogen_name            ETHANE 
_em_vitrification.details                 
;7 sec blotting,
blot force +25
;
_em_vitrification.humidity                100 
_em_vitrification.instrument              'FEI VITROBOT MARK IV' 
_em_vitrification.entry_id                EMD-13008 
_em_vitrification.sample_preparation_id   ? 
_em_vitrification.citation_id             ? 
_em_vitrification.method                  ? 
_em_vitrification.temp                    ? 
_em_vitrification.time_resolved_state     ? 
# 
_em_experiment.entry_id                EMD-13008 
_em_experiment.id                      1 
_em_experiment.aggregation_state       PARTICLE 
_em_experiment.reconstruction_method   'SINGLE PARTICLE' 
_em_experiment.entity_assembly_id      1 
# 
_em_single_particle_entity.entry_id              EMD-13008 
_em_single_particle_entity.id                    1 
_em_single_particle_entity.image_processing_id   1 
_em_single_particle_entity.point_symmetry        C7 
# 
_em_admin.entry_id              EMD-13008 
_em_admin.current_status        REL 
_em_admin.deposition_date       2021-05-27 
_em_admin.deposition_site       PDBE 
_em_admin.details               ? 
_em_admin.last_update           2024-07-17 
_em_admin.map_release_date      2021-09-01 
_em_admin.obsoleted_date        ? 
_em_admin.title                 'Mechanosensitive channel MscS solubilized with LMNG in open conformation with added lipid' 
_em_admin.header_release_date   2021-09-01 
# 
loop_
_em_author_list.ordinal 
_em_author_list.author 
1 'Rasmussen, T.' 
2 'Flegler, V.J.' 
# 
loop_
_em_buffer_component.buffer_id 
_em_buffer_component.id 
_em_buffer_component.concentration 
_em_buffer_component.concentration_units 
_em_buffer_component.formula 
_em_buffer_component.name 
1 1 50   mM    C8H18N2O4S HEPES                                                            
1 2 150  mM    NaCl       'sodium chloride'                                                
1 3 0.02 %     C47H88O22  LMNG                                                             
1 4 0.14 mg/ml ?          '1,2-di-(9,10-dibromo)stearoyl-sn-glycero-3-phosphoethanolamine' 
# 
_em_ctf_correction.id                                1 
_em_ctf_correction.em_image_processing_id            1 
_em_ctf_correction.type                              'PHASE FLIPPING AND AMPLITUDE CORRECTION' 
_em_ctf_correction.details                           ? 
_em_ctf_correction.amplitude_correction              ? 
_em_ctf_correction.amplitude_correction_factor       ? 
_em_ctf_correction.amplitude_correction_space        ? 
_em_ctf_correction.correction_operation              ? 
_em_ctf_correction.phase_reversal                    ? 
_em_ctf_correction.phase_reversal_anisotropic        ? 
_em_ctf_correction.phase_reversal_correction_space   ? 
# 
_em_db_reference.id             1 
_em_db_reference.access_code    7OOA 
_em_db_reference.db_name        PDB 
_em_db_reference.details        ? 
_em_db_reference.relationship   'IN FRAME' 
# 
_em_entity_assembly_molwt.entity_assembly_id   1 
_em_entity_assembly_molwt.id                   1 
_em_entity_assembly_molwt.experimental_flag    NO 
_em_entity_assembly_molwt.units                MEGADALTONS 
_em_entity_assembly_molwt.value                0.224 
# 
_em_entity_assembly_naturalsource.id                   2 
_em_entity_assembly_naturalsource.entity_assembly_id   1 
_em_entity_assembly_naturalsource.cell                 ? 
_em_entity_assembly_naturalsource.cellular_location    ? 
_em_entity_assembly_naturalsource.ncbi_tax_id          409438 
_em_entity_assembly_naturalsource.organ                ? 
_em_entity_assembly_naturalsource.organelle            ? 
_em_entity_assembly_naturalsource.organism             'Escherichia coli SE11' 
_em_entity_assembly_naturalsource.strain               ? 
_em_entity_assembly_naturalsource.tissue               ? 
# 
_em_entity_assembly_recombinant.id                   2 
_em_entity_assembly_recombinant.entity_assembly_id   1 
_em_entity_assembly_recombinant.cell                 ? 
_em_entity_assembly_recombinant.ncbi_tax_id          562 
_em_entity_assembly_recombinant.organism             'Escherichia coli' 
_em_entity_assembly_recombinant.plasmid              pTrc99A 
_em_entity_assembly_recombinant.strain               MJF641 
# 
loop_
_em_euler_angle_assignment.id 
_em_euler_angle_assignment.details 
_em_euler_angle_assignment.image_processing_id 
_em_euler_angle_assignment.order 
_em_euler_angle_assignment.proj_matching_angular_sampling 
_em_euler_angle_assignment.proj_matching_merit_function 
_em_euler_angle_assignment.proj_matching_num_projections 
_em_euler_angle_assignment.type 
1 ? 1 INITIAL ? ? ? 'MAXIMUM LIKELIHOOD' 
2 ? 1 FINAL   ? ? ? 'MAXIMUM LIKELIHOOD' 
# 
_em_final_classification.id                         1 
_em_final_classification.type                       3D 
_em_final_classification.avg_num_images_per_class   ? 
_em_final_classification.details                    ? 
_em_final_classification.image_processing_id        1 
_em_final_classification.num_classes                3 
# 
_em_grid_pretreatment.id                  1 
_em_grid_pretreatment.sample_support_id   1 
_em_grid_pretreatment.atmosphere          air 
_em_grid_pretreatment.pressure            ? 
_em_grid_pretreatment.time                150 
_em_grid_pretreatment.type                'GLOW DISCHARGE' 
# 
_em_image_processing.id                   1 
_em_image_processing.image_recording_id   1 
_em_image_processing.details              ? 
# 
_em_image_recording.id                            1 
_em_image_recording.imaging_id                    1 
_em_image_recording.avg_electron_dose_per_image   80 
_em_image_recording.average_exposure_time         75 
_em_image_recording.details                       ? 
_em_image_recording.detector_mode                 INTEGRATING 
_em_image_recording.film_or_detector_model        'FEI FALCON III (4k x 4k)' 
_em_image_recording.num_diffraction_images        ? 
_em_image_recording.num_grids_imaged              1 
_em_image_recording.num_real_images               10080 
# 
loop_
_em_map.entry_id 
_em_map.id 
_em_map.annotation_details 
_em_map.axis_order_fast 
_em_map.axis_order_medium 
_em_map.axis_order_slow 
_em_map.cell_a 
_em_map.cell_alpha 
_em_map.cell_b 
_em_map.cell_beta 
_em_map.cell_c 
_em_map.cell_gamma 
_em_map.contour_level 
_em_map.contour_level_source 
_em_map.data_type 
_em_map.dimensions_col 
_em_map.dimensions_row 
_em_map.dimensions_sec 
_em_map.endian_type 
_em_map.file 
_em_map.format 
_em_map.label 
_em_map.limit_col 
_em_map.limit_row 
_em_map.limit_sec 
_em_map.origin_col 
_em_map.origin_row 
_em_map.origin_sec 
_em_map.partition 
_em_map.pixel_spacing_x 
_em_map.pixel_spacing_y 
_em_map.pixel_spacing_z 
_em_map.size_kb 
_em_map.spacing_x 
_em_map.spacing_y 
_em_map.spacing_z 
_em_map.statistics_average 
_em_map.statistics_maximum 
_em_map.statistics_minimum 
_em_map.statistics_std 
_em_map.symmetry_space_group 
_em_map.type 
EMD-13008 1 'post-processed: unmasked, sharpened' X Y Z 319.05002 90.0 319.05002 90.0 319.05002 90.0 0.03 author 
'Image stored as floating point number (4 bytes)' 300 300 300 big emd_13008.map.gz            CCP4 
::::EMDATABANK.org::::EMD-13008:::: 299 299 299 0 0 0 1 1.0635 1.0635 1.0635 108001024 300 300 300 -2.9790404E-5 0.40267548 
-0.2138994   0.012008952  1 'primary map' 
EMD-13008 2 mask                                  X Y Z 319.05002 90.0 319.05002 90.0 319.05002 90.0 0.03 author 
'Image stored as floating point number (4 bytes)' 300 300 300 big emd_13008_msk_1.map         CCP4 
::::EMDATABANK.org::::EMD-13008:::: 299 299 299 0 0 0 1 1.0635 1.0635 1.0635 108001024 300 300 300 0.021574039   1.0        0.0 
0.13495594   1 mask          
EMD-13008 3 'unfiltered half-map'                 X Y Z 319.05002 90.0 319.05002 90.0 319.05002 90.0 0.03 author 
'Image stored as floating point number (4 bytes)' 300 300 300 big emd_13008_half_map_1.map.gz CCP4 
::::EMDATABANK.org::::EMD-13008:::: 299 299 299 0 0 0 1 1.0635 1.0635 1.0635 108001024 300 300 300 -3.210925E-5  0.07537355 
-0.025872894 0.0029003923 1 'half map'    
EMD-13008 4 'unfiltered half-map'                 X Y Z 319.05002 90.0 319.05002 90.0 319.05002 90.0 0.03 author 
'Image stored as floating point number (4 bytes)' 300 300 300 big emd_13008_half_map_2.map.gz CCP4 
::::EMDATABANK.org::::EMD-13008:::: 299 299 299 0 0 0 2 1.0635 1.0635 1.0635 108001024 300 300 300 -2.7471562E-5 0.07656651 
-0.024763005 0.0028984302 1 'half map'    
# 
loop_
_em_software.id 
_em_software.category 
_em_software.details 
_em_software.name 
_em_software.version 
_em_software.image_processing_id 
_em_software.fitting_id 
_em_software.imaging_id 
1  'PARTICLE SELECTION'       ? crYOLO  ?     1 ? ? 
2  'PARTICLE SELECTION'       ? RELION  3.1   1 ? ? 
3  'IMAGE ACQUISITION'        ? EPU     ?     ? ? 1 
4  MASKING                    ? ?       ?     ? ? ? 
5  'CTF CORRECTION'           ? CTFFIND 4     1 ? ? 
6  'LAYERLINE INDEXING'       ? ?       ?     ? ? ? 
7  'DIFFRACTION INDEXING'     ? ?       ?     ? ? ? 
8  'MODEL FITTING'            ? Coot    0.9.2 ? 1 ? 
9  OTHER                      ? ?       ?     ? ? ? 
10 'MODEL REFINEMENT'         ? PHENIX  1.19  ? 1 ? 
11 'INITIAL EULER ASSIGNMENT' ? RELION  3.1   1 ? ? 
12 'FINAL EULER ASSIGNMENT'   ? RELION  3.1   1 ? ? 
13 CLASSIFICATION             ? RELION  3.1   1 ? ? 
14 RECONSTRUCTION             ? RELION  3.1   1 ? ? 
# 
_em_specimen.id                      1 
_em_specimen.experiment_id           1 
_em_specimen.concentration           5 
_em_specimen.details                 ? 
_em_specimen.embedding_applied       NO 
_em_specimen.shadowing_applied       NO 
_em_specimen.staining_applied        NO 
_em_specimen.vitrification_applied   YES 
# 
_em_start_model.id                               1 
_em_start_model.details                          ? 
_em_start_model.image_processing_id              1 
_em_start_model.emdb_id                          ? 
_em_start_model.insilico_model                   ? 
_em_start_model.orthogonal_tilt_angle1           ? 
_em_start_model.orthogonal_tilt_angle2           ? 
_em_start_model.orthogonal_tilt_num_images       ? 
_em_start_model.other                            ? 
_em_start_model.pdb_id                           ? 
_em_start_model.random_conical_tilt_angle        ? 
_em_start_model.random_conical_tilt_num_images   ? 
_em_start_model.type                             NONE 
# 
_em_support_film.id                  1 
_em_support_film.sample_support_id   1 
_em_support_film.material            CARBON 
_em_support_film.thickness           ? 
_em_support_film.topology            'HOLEY ARRAY' 
# 
loop_
_pdbx_audit_support.funding_organization 
_pdbx_audit_support.country 
_pdbx_audit_support.grant_number 
_pdbx_audit_support.details 
_pdbx_audit_support.ordinal 
'German Research Foundation (DFG)' Germany Bo1150/15-1          ? 1 
'German Research Foundation (DFG)' Germany 'INST 93/903-1 FUGG' ? 2 
# 
_pdbx_initial_refinement_model.id               1 
_pdbx_initial_refinement_model.type             'experimental model' 
_pdbx_initial_refinement_model.source_name      PDB 
_pdbx_initial_refinement_model.accession_code   6RLD 
# 
