2020-09-30 REL PDBe PDBe PDBe 2020-04-29 2020-06-17 2020-06-17 2020-09-30 German Research Foundation (DFG) research fellowship 385257318 Germany Biotechnology and Biological Sciences Research Council (BBSRC) BB/L023091/1 United Kingdom In situ structure of the Caulobacter crescentus flagellar motor and visualization of binding of a CheY-homolog Rossmann FM Hug I Sangermani M Jenal U Beeby M Rossmann FM Hug I Sangermani M Jenal U Beeby M In situ structure of the Caulobacter crescentus flagellar motor and visualization of binding of a CheY-homolog. Mol.Microbiol. UK 114 443 453 2020 32449846 doi:10.1111/mmi.14525 1365-2958 2007 MOMIEE EMD-10956 associated EM volume Flagellar motor of Caulobactor crescentus CB 15, in situ, cheYs deletion pdeA deletion FliM_ID57WA pMT375 CleD-d6-a-GFP, C100 symmetrised Flagellar motor of Caulobactor crescentus CB 15, in situ, cheYs deletion pdeA deletion FliM_ID57WA pMT375 CleD-d6-a-GFP, C100 symmetrised 0 Caulobacter vibrioides CB15 subtomogramAveraging particle 7 peptone yeast extract; PYE medium UltrAuFoil GOLD 200 GOLD HOLEY 50.0 GLOW DISCHARGE AIR 0.02 ETHANE-PROPANE 100 298 FEI VITROBOT MARK IV
blot time 5 s, blot force 3, wait time 60s, drain time 0.5s,.
FEI TECNAI F20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 100.0 2.0 3.0 4.0 29000.0 GATAN 914 HIGH TILT LIQUID NITROGEN CRYO TRANSFER TOMOGRAPHY HOLDER NITROGEN FEI FALCON II (4k x 4k) INTEGRATING 4096 4096 7 1.5 3.15 1 C100 SIMULTANEOUS ITERATIVE (SIRT) 110.0 FSC 0.5 CUT-OFF PEET 1.10.1 133 127 133 sperical manual PEET 1.10.1 ANGULAR RECONSTITUTION PEET 1.10.1 emd_10956.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 250 250 250 -125 -125 -125 250 250 250 1750.515 1750.515 1750.515 90.0 90.0 90.0 X Y Z -0.53913355 0.9900278 0.0029722117 0.15156096 7.00206 7.00206 7.00206 0.314 AUTHOR Flagellar motor of Caulobactor crescentus CB 15, in situ, cheYs deletion pdeA deletion FliM_ID57WA pMT375 CleD-d6-a-GFP, C100 symmetrised