2020-08-19 REL PDBe PDBe PDBe 2020-04-28 2020-06-24 2020-06-24 2020-08-19 Medical Research Council (MRC, United Kingdom) U105184326 United Kingdom Medical Research Council (MRC, United Kingdom) MC-A652-5PY00 United Kingdom Wellcome Trust 202754/Z/16/Z United Kingdom Condensin complex from S.cerevisiae ATP-free apo bridged state: overall map Lee B-G Cawood C Gutierrez-Escribano P Nakane T Merkel F Hassler M Haering CH Aragon L Lowe J Lee BG Merkel F Allegretti M Hassler M Cawood C Lecomte L O'Reilly FJ Sinn LR Gutierrez-Escribano P Kschonsak M Bravo S Nakane T Rappsilber J Aragon L Beck M Lowe J Haering CH Cryo-EM structures of holo condensin reveal a subunit flip-flop mechanism. Nat.Struct.Mol.Biol. US 27 743 751 2020 32661420 doi:10.1038/s41594-020-0457-x 1545-9985 EMD-10952 other EM volume
focused refined map of head segment
 EMD-10953 other EM volume
focused refined map of arm segment
 EMD-10954 associated EM volume Condensin Condensin 0
complex of 5 protein subunits: Smc2; Smc4; Brn1; Ycs4; Ycg1
Saccharomyces cerevisiae Saccharomyces cerevisiae 500 singleParticle particle 0.2 7.5 Tris ETHANE 100 100 FEI VITROBOT MARK IV
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN VOLTA PHASE PLATE 20 GATAN K2 SUMMIT (4k x 4k) COUNTING 1 10000 10.0 45.0 1 Gctf
Ab initio
 9.1 FSC 0.143 CUT-OFF RELION 37693 ANGULAR RECONSTITUTION RELION ANGULAR RECONSTITUTION RELION emd_10954.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 380 380 380 0 0 0 380 380 380 456.00003 456.00003 456.00003 90.0 90.0 90.0 X Y Z -0.04741448 0.1374229 0.0000431209 0.0060814815 1.2 1.2 1.2 0.0444 AUTHOR 4RSI A 4RSI B 6QJ2 A 6QJ2 B 6CJ3 A 6C63 A 6Q6E RIGID BODY FIT REAL