2021-05-26 REL PDBe PDBe PDBe 2020-04-25 2020-11-11 2020-11-11 2021-05-26 European Research Council (ERC) AdG 340227 Switzerland Sub-tomograms average of the Chlamydomonas reinhardtii CrSAS-6[NL] in vitro stacked cartwheels Nazarov S Hatzopoulos G Nazarov S Bezler A Hatzopoulos GN Nemcikova Villimova V Demurtas D Le Guennec M Guichard P Gonczy P Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography. Embo J. UK 39 e106249 e106249 2020 32954505 doi:10.15252/embj.2020106249 1460-2075 0897 EMJODG EMD-10916 other EM volume EMD-10918 other EM volume EMD-10922 other EM volume EMD-10923 other EM volume EMD-10927 other EM volume EMD-10928 associated EM volume Expressed and purified CrSAS-6[NL] (N-terminal globular head domain plus the entire coiled-coil domain) Expressed and purified CrSAS-6[NL] (N-terminal globular head domain plus the entire coiled-coil domain) 0 Chlamydomonas reinhardtii Escherichia coli BL21(DE3) subtomogramAveraging threeDArray 7.5 Quantifoil R1.2/1.3 COPPER 300 CARBON LACEY GLOW DISCHARGE ETHANE FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 1.83 1 C9 25.0 FSC 0.143 CUT-OFF RELION 3.1 334 31 926 RELION 3.1 IMOD 4.9 RELION 3.1 MAXIMUM LIKELIHOOD RELION 3.1 emd_10928.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 128 128 128 0 0 0 128 128 128 693.76 693.76 693.76 90.0 90.0 90.0 X Y Z -0.05995535 0.10874389 0.00027689637 0.00778006 5.42 5.42 5.42 0.015 AUTHOR