2021-05-26 REL PDBe PDBe PDBe 2020-04-17 2020-08-05 2020-08-05 2021-05-26 Biotechnology and Biological Sciences Research Council (BBSRC) BB/P020208/1 United Kingdom Biotechnology and Biological Sciences Research Council (BBSRC) BB/P026397/1 United Kingdom Wellcome Trust 108466/Z/15/Z United Kingdom HSPD1 single ring deposited by spraying (50 ms delay) Klebl DP Gravett MSC Darrow M Thompson RF Muench SP Klebl DP Gravett MSC Kontziampasis D Wright DJ Bon RS Monteiro DCF Trebbin M Sobott F White HD Darrow MC Thompson RF Muench SP Need for Speed: Examining Protein Behavior during CryoEM Grid Preparation at Different Timescales. Structure UK 28 1238 1248.e4 2020 32814033 doi:10.1016/j.str.2020.07.018 0969-2126 2005 STRUE6 EMD-10884 associated EM volume human mitochondrial 60 kDa heat shock protein (mature) human mitochondrial 60 kDa heat shock protein (mature) 0 1 Homo sapiens Escherichia coli human mitochondrial 60 kDa heat shock protein (mature) Homo sapiens Escherichia coli LEVO SNAAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSIQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLTPANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEVGYDAMAGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGAMGGMGGGMGGGMF singleParticle particle 4.5 8.0 ETHANE 80 293 HOMEMADE PLUNGER
the sample was sprayed using a microfludic nozzle, resulting in a delay of 50ms between spraying and freezing.
FEI TITAN KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 FEI FALCON III (4k x 4k) INTEGRATING 1.5 81.0 1 C7 6.8 FSC 0.143 CUT-OFF RELION 39610 NOT APPLICABLE MAXIMUM LIKELIHOOD RELION
The final angular assignment was done in a bigger, combined dataset which was then split into the original datasets to generate this reconstruction
 emd_10884.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 124 124 124 0 0 0 124 124 124 264.12003 264.12003 264.12003 90.0 90.0 90.0 X Y Z -0.08343847 0.25357452 0.00014410625 0.015647853 2.13 2.13 2.13 0.083 AUTHOR unsharpened final map emd_10884_msk_1.map emd_10884_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 124 124 124 0 0 0 124 124 124 264.12003 264.12003 264.12003 90.0 90.0 90.0 X Y Z -0.042589013 0.13558055 0.00015501585 0.013387605 2.13 2.13 2.13 AUTHOR emd_10884_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 124 124 124 0 0 0 124 124 124 264.12003 264.12003 264.12003 90.0 90.0 90.0 X Y Z -0.042620137 0.13239376 0.00013319665 0.013379194 2.13 2.13 2.13 AUTHOR