REL PDBe PDBe PDBe 2004-07-01 2004-07-07 2004-09-07 2013-09-25 Structure of the acrosomal bundle. Schmid MF Sherman MB Matsudaira P Chiu W Schmid MF Sherman MB Matsudaira P Chiu W Structure of the acrosomal bundle. NATURE 431 104 107 2004 15343340 doi:10.1038/nature02881 3b5u FULLOVERLAP 3b63 FULLOVERLAP Acrosomal bundle from Limulus sperm Acrosomal bundle from Limulus sperm helix of actin and scruin in a 1 to 1 stoichiometry 2 4.2 4.2 calculated from molecular weight actin Limulus polyphemus horseshoe crab sperm sperm acrosomal process acrosomal bundle 1.176 1.176
28 of these per unit cell
28 helical false scruin Limulus polyphemus horseshoe crab sperm sperm acrosomal process acrosomal bundle 3.08 3.08
28 of these per unit cell
28 helical false helical twoDArray 10 7.4
see J Mol Biol, 221: 711-725 (1991)
 ETHANE 100 90 HOMEMADE PLUNGER
Vitrification instrument: homemade guillotine
naturally occurring intracellular ordered array. Space group is P2sub1 or P1 21 1. P 1 21 refers to a 2-fold screw axis in the plane of the 2D crystal, whereas I have defined the 2-fold screw along z (second setting monoclinic).
 JEOL 4000EX FLOOD BEAM BRIGHT FIELD LAB6 400 0.8 3.0 40000.0 GATAN LIQUID NITROGEN 90 KODAK SO-163 FILM ZEISS SCAI 7 90 15
153 bundles selected
1.5 side entry OTHER 9.5 OTHER MRC, CCP4, homemade
1000 2 unit cell segments from 153 bundles (ref:Schmid, MF, J,. Struct Biol. 2003, 144:195-208.
each bundle
 145 145 765 120 90 90 P 1 21 emd_1088.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 576 192 112 -288 -96 -56 112 192 576 255.99936 148.00015 765.7977 90.0 90.0 90.0 Z X Y -974.402832030000013 977.198852539999962 -0.00000001 215.096801760000005 1.33333 1.32143 1.32951 430.0 AUTHOR One unit cell of the acrosomal bundle, pseudo-centered to provide a orthogonal map from the pseudo-hexagonal packing
::::EMDATABANK.org::::EMD-1088::::
 1ATN foldhunter and homemade software
Protocol: cross-correlation. each of the 28 actins was fitted and averaged, scruin was density-correlated and averaged because there is no crystal structure. overall B is the NEGATIVE B factor applied as a high-pass filter in the calculation of the map (-500).
cross-correlation REAL -500.0