2020-09-30 REL PDBe PDBe PDBe 2020-03-21 2020-04-01 2020-04-01 2020-09-30 German Research Foundation (DFG) FOR2092 (EN 1194/1-1) Germany In situ cryo-electron tomogram of the Chlamydomonas chloroplast (Volta phase plate, bin4) Schaffer M Wietrzynski W Tegunov D Albert S Plitzko J Baumeister W Engel BD Wietrzynski W Schaffer M Tegunov D Albert S Kanazawa A Plitzko JM Baumeister W Engel BD Charting the native architecture of Chlamydomonas thylakoid membranes with single-molecule precision. Elife US 9 2020 32297859 doi:10.7554/eLife.53740 2050-084X EMD-10780 associated EM volume EMD-10783 other EM volume EMD-10782 other EM volume EMD-10781 other EM volume Whole Chlamydomonas cells Whole Chlamydomonas cells 0
Grown suspended in TAP media, with normal atmosphere aeration and constant light
Chlamydomonas reinhardtii mat3-4 tomography cell 7
TAP media
 Quantifoil R2/1 COPPER 200 CARBON HOLEY GLOW DISCHARGE AIR ETHANE-PROPANE FEI VITROBOT MARK IV
blotted from back for 10 seconds before plunging.
OTHER OTHER 30 0.03 2000 95 1000 75
See https://bio-protocol.org/e1575 for detailed procedure.. The value given for _emd_sectioning_focused_ion_beam.instrument is FEI Quanta FIB. This is not in a list of allowed values set(['DB235', 'OTHER']) so OTHER is written into the XML file.
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 0.5 0.5 42000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN VOLTA PHASE PLATE GIF Quantum LS 20
Bidirectional tilt scheme, separated at 0 degrees, Volta Phase Plate
GATAN K2 SUMMIT (4k x 4k) COUNTING 61 1.5 1.5
Images were collected in movie-mode at 12 frames per second
 1 BACK PROJECTION IMOD 61 IMOD emd_10780.map.gz 1 IMAGE STORED AS SIGNED INTEGER (2 BYTES) 928 928 464 0 0 232 928 928 464 12695.04 12695.04 6347.52 90.0 90.0 90.0 X Y Z -162 131 5.400458 10.212014 13.68 13.68 13.68 AUTHOR In situ cryo-electron tomogram of the Chlamydomonas chloroplast (Volta phase plate, bin4)