Cryo-EM structure of norovirus GII.4c VLPs with T=3 icosahedral symmetry

2021-04-14 REL PDBe PDBe PDBe 2020-03-12 2020-11-04 2020-11-04 2021-04-14 Cryo-EM structure of norovirus GII.4c VLPs with T=3 icosahedral symmetry Devant JM Hansman GS Devant JM Hansman GS Structural heterogeneity of a human norovirus vaccine candidate. Virology US 553 23 34 2020 33202318 doi:10.1016/j.virol.2020.10.005 0042-6822 0922 VIRLAX EMD-10755 other EM volume

GII.4c T=4 particle

EMD-10757 other EM volume

GII.4c T=1 particle

EMD-10758 other EM volume

GII.4c from HEK293T cells, T=4 particle

EMD-10759 other EM volume

GII.17 VLPs, T=3 particle

EMD-10756 associated EM volume Norovirus 06-AM-11/2006/GII.4/Yerseke/2006a Norovirus 06-AM-11/2006/GII.4/Yerseke/2006a 0 Norovirus 06-AM-11/2006/GII.4/Yerseke/2006a GII.4c Homo sapiens Trichoplusia ni HighFive insect cells 460.0 3 VIRUS-LIKE PARTICLE STRAIN false true singleParticle particle 2 7.4 PBS

PBS buffer

ETHANE 100 285 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 64000.0 FEI TITAN KRIOS AUTOGRID HOLDER GATAN K3 (6k x 4k) 2 3983 15.0 1 CTFFIND 4 I 8.05 FSC 0.143 CUT-OFF cryoSPARC 2.4.0 44317 MAXIMUM LIKELIHOOD cryoSPARC 2.4.0 MAXIMUM LIKELIHOOD cryoSPARC 2.4.0 emd_10756.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 550.0 550.0 550.0 90.0 90.0 90.0 X Y Z -0.7340676 1.0907187 0.020550592 0.081852846 1.375 1.375 1.375 0.164 AUTHOR ::::EMDATABANK.org::::EMD-10756:::: Cryo-EM structure of GII.4c VLPs with T=3 icosahedral symmmetry