2020-11-18 REL PDBe PDBe PDBe 2020-03-03 2020-03-11 2020-09-16 2020-11-18 European Research Council (ERC) ERC-AdG-2012-321295 Switzerland Human Frontier Science Program (HFSP) LT000971/2016 Switzerland OP protein cage Edwardson TGW Tetter S Hilvert D Edwardson TGW Tetter S Hilvert D Two-tier supramolecular encapsulation of small molecules in a protein cage. Nat Commun UK 11 5410 5410 2020 33106476 doi:10.1038/s41467-020-19112-1 2041-1723 EMD-10723 associated EM volume OP protein cage OP protein cage 0 1
Positively supercharged variant of the computationally designed cage protein O3-33
Salmonella enterica Escherichia coli BL21(DE3) OP protein cage Salmonella enterica Escherichia coli BL21(DE3) LEVO MSQAIGILELRSIAAGMELGDAMLKSANVDLLVSKTISRGKFLLMLGGDIGAIQQAIETGTSQAGRLLVDSLVLANIHPSVLPAISGLNSVDKRQAVGIVETRSVAACISAADRAVKGSNVTLVRVHMARGIGGKCYMVVAGDVSDVALAVTVASSSAGAYGRLVYASLIPRPHEAMWRQMVEGLEHHHHHH singleParticle particle 7.4
25 mM Tris-HCl, 200 mM NaCl, 5 mM EDTA, pH 7.4
 Quantifoil R2/2 COPPER 400 CARBON HOLEY GLOW DISCHARGE AIR ETHANE 100 22
blot for 12 s, 25 blot strength, before plunging.
FEI TECNAI F20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.0 -1.8 -3.3 62000.0 NITROGEN FEI FALCON II (4k x 4k) INTEGRATING 4096 4096 1-7 1 77 30.0 1 45328 Gctf 1.06
The initial model was generated from the data themselves, with octahedral symmetry imposed.
 1 O 8.8 FSC 0.143 CUT-OFF RELION 3.0 2516 MAXIMUM LIKELIHOOD RELION 3.0 MAXIMUM LIKELIHOOD RELION 3.0 3 1374 RELION 3.0 emd_10723.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 180 180 180 0 0 0 180 180 180 324.0 324.0 324.0 90.0 90.0 90.0 X Y Z -0.04927162 0.158398 0.00089419476 0.01401826 1.8 1.8 1.8 0.0857 AUTHOR OP map, postprocessed 6FDB RIGID BODY FIT Correlation coefficient REAL emd_10723_msk_1.map emd_10723_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 180 180 180 0 0 0 180 180 180 324.0 324.0 324.0 90.0 90.0 90.0 X Y Z -0.034034055 0.11695133 0.00087966933 0.012132581 1.8 1.8 1.8 AUTHOR OP, half map 2 emd_10723_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 180 180 180 0 0 0 180 180 180 324.0 324.0 324.0 90.0 90.0 90.0 X Y Z -0.037606202 0.114905246 0.0009087202 0.012161262 1.8 1.8 1.8 AUTHOR OP, half map 1