2020-06-10 REL PDBe PDBe PDBe 2020-03-02 2020-06-10 2020-06-10 2020-06-10 French National Research Agency ANR-14CE09-0003-01 France Cryo-tomography of ESCRT III CHMP4B/CHMP2B filaments bound to liposomes Bertin A de Franceschi N de la Mora E Maity S Miguet N di Cicco A Roos W Mangenot S Weissenhorn W Bassereau P Bertin A de Franceschi N de la Mora E Maiti S Alqabandi M Miguet N di Cicco A Roos WH Mangenot S Weissenhorn W Bassereau P Human ESCRT-III polymers assemble on positively curved membranes and induce helical membrane tube formation. Nat Commun UK 11 2663 2663 2020 32471988 doi:10.1038/s41467-020-16368-5 2041-1723 EMD-10720 associated EM volume CHMP4B, CHMP2B, lipids CHMP4B, CHMP2B, lipids 0 1 2 Homo sapiens Escherichia coli CHMP4B DEXTRO MSVFGKLFGA GGGKAGKGGP TPQEAIQRLR DTEEMLSKKQ EFLEKKIEQE LTAAKKHGTK NKRAALQALK RKKRYEKQLA QIDGTLSTIE FQREALENAN TNTEVLKNMG YAAKAMKAAH DNMDIDKVDE LMQDIADQQE LAEEISTAIS KPVGFGEEFD EDELMAELEE LEQEELDKNL LEISGPETVP LPNVPSIALP SKPAKKKEEE DDDMKELENW AGSM CHMP2B DEXTRO MASLFKKKTV DDVIKEQNRE LRGTQRAIIR DRAALEKQEK QLELEIKKMA KIGNKEACKV LAKQLVHLRK QKTRTFAVSS KVTSMSTQTK VMNSQMKMAG AMSTTAKTMQ AVNKKMDPQK TLQTMQNFQK ENMKMEMTEE MINDTLDDIF DGSDDEEESQ DIVNQVLDEI GIEISGKMAK APSAARSLPS ASTSKATISD EEIERQLKAL GVD tomography twoDArray 0.1 8 ETHANE
protein filaments bound to liposomes
Sigma Aldrich 10 NO SECTIONING FEI TITAN KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 QUANTUM (4k x 4k) 5.0 1 41 emd_10720.map.gz 1 IMAGE STORED AS SIGNED INTEGER (2 BYTES) 960 928 361 17 180 -16 960 928 361 5088.0 4918.4004 1913.3 90.0 90.0 90.0 X Y Z -94 86 0.55204535 6.837252 5.3 5.3 5.3 AUTHOR example cryo-tomogram of ESCRT III bound to liposomes emd_10720_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 64 64 64 0 0 0 64 64 64 339.2 339.2 339.2 90.0 90.0 90.0 X Y Z -5.1683226 4.2819877 -0.23481329 0.84556717 5.3 5.3 5.3 AUTHOR sub-tomogram averaging: doublets of CHMP4/CHMP2B filaments bound to tubes emd_10720_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 64 64 64 0 0 0 64 64 64 339.2 339.2 339.2 90.0 90.0 90.0 X Y Z -4.7495594 4.8283286 -0.0838363 0.68379176 5.3 5.3 5.3 AUTHOR sub-tomogram averaging: network of CHMP4/CHMP2B filaments bound to tubes emd_10720_additional_3.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 64 64 64 0 0 0 64 64 64 339.2 339.2 339.2 90.0 90.0 90.0 X Y Z -4.604772 4.075515 -0.11751016 0.6852304 5.3 5.3 5.3 AUTHOR sub-tomogram averaging: single CHMP4/CHMP2B filaments bound to tubes