2020-03-18 REL PDBe PDBe PDBe 2020-02-28 2020-03-18 2020-03-18 2020-03-18 French National Research Agency ANR 16-CE11-0029 France head domain of Tsr1-FPZ pre-40S particle: structural class 2 (H2) Shayan R Plassart L Plisson-Chastang C Shayan R Rinaldi D Larburu N Plassart L Balor S Bouyssie D Lebaron S Marcoux J Gleizes PE Plisson-Chastang C Good Vibrations: Structural Remodeling of Maturing Yeast Pre-40S Ribosomal Particles Followed by Cryo-Electron Microscopy. Molecules CH 25 2020 32138239 doi:10.3390/molecules25051125 1420-3049 EMD-10713 other EM volume EMD-10715 other EM volume EMD-10716 other EM volume EMD-10717 associated EM volume early cytoplasmic yeast pre-40S particle (purified using Tsr1 as bait) early cytoplasmic yeast pre-40S particle (purified using Tsr1 as bait) 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 Saccharomyces cerevisiae S288C 1.8 singleParticle particle 7.4 Quantifoil R2/1 COPPER 300 CARBON CONTINUOUS 2.0 GLOW DISCHARGE ETHANE 95 292 LEICA EM GP
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) 29.4 1 4V88 1 C1 6.4 FSC 0.143 CUT-OFF RELION 2.1 15065 MAXIMUM LIKELIHOOD RELION 2.1 MAXIMUM LIKELIHOOD RELION 2.1 RELION 2.1 emd_10717.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 384.12003 384.12003 384.12003 90.0 90.0 90.0 X Y Z -0.099766865 0.3230441 0.0015132958 0.011385999 1.067 1.067 1.067 0.065 AUTHOR H2-Tsr1 pre-40S Head of yeast Tsr1-FPZ pre-40S particle Structural class 2 6FAI OTHER emd_10717_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 384.12003 384.12003 384.12003 90.0 90.0 90.0 X Y Z -0.028745744 0.12595688 0.00054193527 0.0057024 1.067 1.067 1.067 AUTHOR H2-Tsr1 pre-40S Head of yeast Tsr1-FPZ pre-40S particle Structural class 2 Unfiltered half-map 1 emd_10717_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 384.12003 384.12003 384.12003 90.0 90.0 90.0 X Y Z -0.029947128 0.12143761 0.000492055 0.005739533 1.067 1.067 1.067 AUTHOR H2-Tsr1 pre-40S Head of yeast Tsr1-FPZ pre-40S particle Structural class 2 Unfiltered half-map 2