2021-04-14 REL PDBe PDBe PDBe 2020-02-20 2020-08-05 2020-08-05 2021-04-14 European Research Council (ERC) 760067 Germany in-cell elongating expressome from Mycoplasma pneumoniae Mahamid J Xue L O'Reilly FJ Xue L Graziadei A Sinn L Lenz S Tegunov D Blotz C Singh N Hagen WJH Cramer P Stulke J Mahamid J Rappsilber J In-cell architecture of an actively transcribing-translating expressome. Science US 369 554 557 2020 32732422 doi:10.1126/science.abb3758 1095-9203 0038 SCIEAS EMD-10679 associated EM volume wild-type Mycoplasma pneumoniae M129 cells wild-type Mycoplasma pneumoniae M129 cells 0 Mycoplasma pneumoniae M129 subtomogramAveraging cell 7.4
modified Hayflick medium as described in Halbedel, Hames, and Stulke 2004
 Quantifoil R2/1 GOLD CARBON HOLEY GLOW DISCHARGE ETHANE-PROPANE 45 HOMEMADE PLUNGER FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 1.5 4.5 81000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 2.9 1 C1 8.5 FSC 0.143 CUT-OFF RELION 3.0.7
Refinements performed with spherical mask generated in RELION. Postprocessing performed with tight mask.
2952 352 73858 Warp 1.0.6 Warp 1.0.6 RELION 3.0.7 MAXIMUM LIKELIHOOD RELION 3.0.7 emd_10679.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 680.2 680.2 680.2 90.0 90.0 90.0 X Y Z -1.4960692 3.3035793 0.015047343 0.14095443 3.401 3.401 3.401 0.75 AUTHOR In-cell expressome from Mycoplasma pneumoniae emd_10679_msk_1.map emd_10679_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 680.2 680.2 680.2 90.0 90.0 90.0 X Y Z -2.002732 3.4257786 0.007624978 0.3080191 3.401 3.401 3.401 AUTHOR In-cell expressome from Mycoplasma pneumoniae, half 1 emd_10679_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 680.2 680.2 680.2 90.0 90.0 90.0 X Y Z -2.2642758 3.3734725 0.00799414 0.31255493 3.401 3.401 3.401 AUTHOR In-cell expressome from Mycoplasma pneumoniae, half 1