data_EMD-10676
# 
_entry.id   EMD-10676 
# 
_audit_conform.dict_name       mmcif_pdbx.dic 
_audit_conform.dict_version    5.399 
_audit_conform.dict_location   http://mmcif.pdb.org/dictionaries/ascii/mmcif_pdbx.dic 
# 
loop_
_database_2.database_id 
_database_2.database_code 
_database_2.pdbx_database_accession 
_database_2.pdbx_DOI 
PDB   6Y3Y         pdb_00006y3y 10.2210/pdb6y3y/pdb 
WWPDB D_1292106689 ?            ?                   
EMDB  EMD-10676    ?            ?                   
# 
_pdbx_database_related.db_name        EMDB 
_pdbx_database_related.details        'Human Coronavirus HKU1 Haemagglutinin-Esterase' 
_pdbx_database_related.db_id          EMD-10676 
_pdbx_database_related.content_type   'associated EM volume' 
# 
loop_
_citation.abstract 
_citation.abstract_id_CAS 
_citation.book_id_ISBN 
_citation.book_publisher 
_citation.book_publisher_city 
_citation.book_title 
_citation.coordinate_linkage 
_citation.country 
_citation.database_id_Medline 
_citation.details 
_citation.id 
_citation.journal_abbrev 
_citation.journal_id_ASTM 
_citation.journal_id_CSD 
_citation.journal_id_ISSN 
_citation.journal_full 
_citation.journal_issue 
_citation.journal_volume 
_citation.language 
_citation.page_first 
_citation.page_last 
_citation.title 
_citation.year 
_citation.database_id_CSD 
_citation.pdbx_database_id_DOI 
_citation.pdbx_database_id_PubMed 
_citation.unpublished_flag 
? ? ? ? ? ? ? UK ? ? primary 'Nat Commun' ? ? 2041-1723 ? ? 11 ? 4646 4646 
;Cryo-EM structure of coronavirus-HKU1 haemagglutinin esterase reveals architectural changes arising from prolonged circulation in humans.
;
2020 ? 10.1038/s41467-020-18440-6 32938911 ? 
? ? ? ? ? ? ? US ? ? 1       Biorxiv      ? ? 2692-8205 ? ? ?  ? ?    ?    
;Cryo-EM structure of coronavirus-HKU1 haemagglutinin esterase reveals architectural changes arising from prolonged circulation in humans
;
2020 ? 10.1101/2020.03.25.998963  ?        ? 
# 
loop_
_citation_author.citation_id 
_citation_author.name 
_citation_author.ordinal 
_citation_author.identifier_ORCID 
primary 'Hurdiss, D.L.'         1  0000-0003-3834-5808 
primary 'Drulyte, I.'           2  ?                   
primary 'Lang, Y.'              3  ?                   
primary 'Shamorkina, T.M.'      4  ?                   
primary 'Pronker, M.F.'         5  0000-0001-5268-7690 
primary 'van Kuppeveld, F.J.M.' 6  0000-0001-5800-749X 
primary 'Snijder, J.'           7  ?                   
primary 'de Groot, R.J.'        8  ?                   
1       'Hurdiss, D.L.'         9  0000-0003-3834-5808 
1       'Drulyte, I.'           10 0000-0003-1117-7699 
1       'Lang, Y.'              11 0000-0002-8179-6471 
1       'Shamorkina, T.M.'      12 0000-0002-4191-6061 
1       'Pronker, M.F.'         13 0000-0001-5268-7690 
1       'van Kuppeveld, F.J.M.' 14 ?                   
1       'Snijder, J.'           15 ?                   
1       'de Groot, R.J.'        16 ?                   
# 
loop_
_entity.id 
_entity.type 
_entity.src_method 
_entity.pdbx_description 
_entity.formula_weight 
_entity.pdbx_number_of_molecules 
_entity.pdbx_ec 
_entity.pdbx_mutation 
_entity.pdbx_fragment 
_entity.details 
1 polymer     man Hemagglutinin-esterase 40432.648 2 3.1.1.53 ? ? ? 
2 branched    man 
;alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
;
748.682   2 ?        ? ? ? 
3 branched    man 
;alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
;
910.823   2 ?        ? ? ? 
4 branched    man 
'beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose' 586.542   2 ? 
? ? ? 
5 branched    man '2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose' 424.401   2 ?        ? 
? ? 
6 non-polymer man 2-acetamido-2-deoxy-beta-D-glucopyranose 221.208   6 ?        ? ? ? 
# 
_entity_poly.entity_id                  1 
_entity_poly.type                       'polypeptide(L)' 
_entity_poly.pdbx_seq_one_letter_code   
;LAFNEPLNVVSHLNHDWFLFGDSRSDCNHINNLKIKNFDYLDIHPSLCNNGKISSSAGDSIFKSFHFTRFYNYTGEGDQI
IFYEGVNFNPYHRFKCFPNGSNDVWLLNKVRFYRALYSNMAFFRYLTFVDIPYNVSLSKFNSCKSDILSLNNPIFINYSK
EVYFTLLGCSLYLVPLCLFKSNFSQYYYNIDTGSVYGFSNVVYPDLDCIYISLKPGSYKVSTTAPFLSLPTKALCFDKSK
QFVPVQVVDSRWNNERASDISLSVACQLPYCYFRNSSANYVGKYDINHGDSGFISILSGLLYNVSCISYYGVFLYDNFTS
IWPYYSFGRCPTSSIIKHPICVYDSDPLVPR
;
# 
_pdbx_entity_nonpoly.entity_id   6 
_pdbx_entity_nonpoly.name        2-acetamido-2-deoxy-beta-D-glucopyranose 
_pdbx_entity_nonpoly.comp_id     NAG 
# 
_entity_src_gen.entity_id                          1 
_entity_src_gen.pdbx_src_id                        1 
_entity_src_gen.pdbx_alt_source_flag               sample 
_entity_src_gen.pdbx_seq_type                      'Biological sequence' 
_entity_src_gen.pdbx_beg_seq_num                   1 
_entity_src_gen.pdbx_end_seq_num                   351 
_entity_src_gen.gene_src_common_name               HCoV-HKU1 
_entity_src_gen.gene_src_genus                     ? 
_entity_src_gen.pdbx_gene_src_gene                 'HE, 2' 
_entity_src_gen.gene_src_species                   ? 
_entity_src_gen.gene_src_strain                    ? 
_entity_src_gen.gene_src_tissue                    ? 
_entity_src_gen.gene_src_tissue_fraction           ? 
_entity_src_gen.gene_src_details                   ? 
_entity_src_gen.pdbx_gene_src_fragment             ? 
_entity_src_gen.pdbx_gene_src_scientific_name      'Human coronavirus HKU1' 
_entity_src_gen.pdbx_gene_src_ncbi_taxonomy_id     290028 
_entity_src_gen.pdbx_gene_src_variant              ? 
_entity_src_gen.pdbx_gene_src_cell_line            ? 
_entity_src_gen.pdbx_gene_src_atcc                 ? 
_entity_src_gen.pdbx_gene_src_culture_collection   ? 
_entity_src_gen.pdbx_gene_src_organ                ? 
_entity_src_gen.pdbx_gene_src_organelle            ? 
_entity_src_gen.pdbx_gene_src_cell                 ? 
_entity_src_gen.pdbx_gene_src_cellular_location    ? 
_entity_src_gen.host_org_common_name               Human 
_entity_src_gen.pdbx_host_org_scientific_name      'Homo sapiens' 
_entity_src_gen.pdbx_host_org_ncbi_taxonomy_id     9606 
_entity_src_gen.host_org_genus                     ? 
_entity_src_gen.pdbx_host_org_gene                 ? 
_entity_src_gen.pdbx_host_org_organ                ? 
_entity_src_gen.host_org_species                   ? 
_entity_src_gen.pdbx_host_org_tissue               ? 
_entity_src_gen.pdbx_host_org_tissue_fraction      ? 
_entity_src_gen.pdbx_host_org_strain               ? 
_entity_src_gen.pdbx_host_org_variant              ? 
_entity_src_gen.pdbx_host_org_cell_line            HEK293T 
_entity_src_gen.pdbx_host_org_atcc                 CRL-3216 
_entity_src_gen.pdbx_host_org_culture_collection   ? 
_entity_src_gen.pdbx_host_org_cell                 ? 
_entity_src_gen.pdbx_host_org_organelle            ? 
_entity_src_gen.pdbx_host_org_cellular_location    ? 
_entity_src_gen.pdbx_host_org_vector_type          ? 
_entity_src_gen.pdbx_host_org_vector               ? 
_entity_src_gen.host_org_details                   ? 
_entity_src_gen.expression_system_id               ? 
_entity_src_gen.plasmid_name                       ? 
_entity_src_gen.plasmid_details                    ? 
_entity_src_gen.pdbx_description                   ? 
# 
loop_
_chem_comp.id 
_chem_comp.type 
_chem_comp.mon_nstd_flag 
_chem_comp.name 
_chem_comp.pdbx_synonyms 
_chem_comp.formula 
_chem_comp.formula_weight 
ALA 'L-peptide linking'           y ALANINE                                  ? 'C3 H7 N O2'     89.093  
ARG 'L-peptide linking'           y ARGININE                                 ? 'C6 H15 N4 O2 1' 175.209 
ASN 'L-peptide linking'           y ASPARAGINE                               ? 'C4 H8 N2 O3'    132.118 
ASP 'L-peptide linking'           y 'ASPARTIC ACID'                          ? 'C4 H7 N O4'     133.103 
BMA 'D-saccharide, beta linking'  . beta-D-mannopyranose                     'beta-D-mannose; D-mannose; mannose' 'C6 H12 O6'      
180.156 
CYS 'L-peptide linking'           y CYSTEINE                                 ? 'C3 H7 N O2 S'   121.158 
GLN 'L-peptide linking'           y GLUTAMINE                                ? 'C5 H10 N2 O3'   146.144 
GLU 'L-peptide linking'           y 'GLUTAMIC ACID'                          ? 'C5 H9 N O4'     147.129 
GLY 'peptide linking'             y GLYCINE                                  ? 'C2 H5 N O2'     75.067  
HIS 'L-peptide linking'           y HISTIDINE                                ? 'C6 H10 N3 O2 1' 156.162 
ILE 'L-peptide linking'           y ISOLEUCINE                               ? 'C6 H13 N O2'    131.173 
LEU 'L-peptide linking'           y LEUCINE                                  ? 'C6 H13 N O2'    131.173 
LYS 'L-peptide linking'           y LYSINE                                   ? 'C6 H15 N2 O2 1' 147.195 
MAN 'D-saccharide, alpha linking' . alpha-D-mannopyranose                    'alpha-D-mannose; D-mannose; mannose' 'C6 H12 O6' 
180.156 
MET 'L-peptide linking'           y METHIONINE                               ? 'C5 H11 N O2 S'  149.211 
NAG 'D-saccharide, beta linking'  . 2-acetamido-2-deoxy-beta-D-glucopyranose 
;N-acetyl-beta-D-glucosamine; 2-acetamido-2-deoxy-beta-D-glucose; 2-acetamido-2-deoxy-D-glucose; 2-acetamido-2-deoxy-glucose; N-ACETYL-D-GLUCOSAMINE
;
'C8 H15 N O6'    221.208 
PHE 'L-peptide linking'           y PHENYLALANINE                            ? 'C9 H11 N O2'    165.189 
PRO 'L-peptide linking'           y PROLINE                                  ? 'C5 H9 N O2'     115.130 
SER 'L-peptide linking'           y SERINE                                   ? 'C3 H7 N O3'     105.093 
THR 'L-peptide linking'           y THREONINE                                ? 'C4 H9 N O3'     119.119 
TRP 'L-peptide linking'           y TRYPTOPHAN                               ? 'C11 H12 N2 O2'  204.225 
TYR 'L-peptide linking'           y TYROSINE                                 ? 'C9 H11 N O3'    181.189 
VAL 'L-peptide linking'           y VALINE                                   ? 'C5 H11 N O2'    117.146 
# 
_exptl.entry_id         EMD-10676 
_exptl.details          ? 
_exptl.method           'ELECTRON MICROSCOPY' 
_exptl.method_details   ? 
# 
_struct_keywords.entry_id        EMD-10676 
_struct_keywords.text            'Coronavirus, Glycoprotein, Nidovirus, Esterase, VIRAL PROTEIN' 
_struct_keywords.pdbx_keywords   'VIRAL PROTEIN' 
_struct_keywords.pdbx_details    ? 
# 
_struct_ref.id          1 
_struct_ref.db_name     UNP 
_struct_ref.db_code     HEMA_CVHN1 
_struct_ref.entity_id   1 
# 
_em_3d_fitting.entry_id          EMD-10676 
_em_3d_fitting.id                1 
_em_3d_fitting.details           'A homology model of HCOV-HKU1 HE (uniprot ID: Q5MQD1) was generated using the phyre2 server' 
_em_3d_fitting.overall_b_value   ? 
_em_3d_fitting.ref_protocol      'RIGID BODY FIT' 
_em_3d_fitting.ref_space         REAL 
_em_3d_fitting.target_criteria   'Correlation coefficient' 
_em_3d_fitting.method            ? 
# 
_em_3d_reconstruction.entry_id                    EMD-10676 
_em_3d_reconstruction.id                          1 
_em_3d_reconstruction.algorithm                   'BACK PROJECTION' 
_em_3d_reconstruction.details                     ? 
_em_3d_reconstruction.fsc_type                    'EVEN/ODD MAPS REFINED TOTALLY INDEPENDENT (GOLD STANDARD)' 
_em_3d_reconstruction.refinement_type             ? 
_em_3d_reconstruction.image_processing_id         1 
_em_3d_reconstruction.num_class_averages          ? 
_em_3d_reconstruction.num_particles               119717 
_em_3d_reconstruction.resolution                  3.39 
_em_3d_reconstruction.resolution_method           'FSC 0.143 CUT-OFF' 
_em_3d_reconstruction.symmetry_type               POINT 
_em_3d_reconstruction.method                      ? 
_em_3d_reconstruction.nominal_pixel_size          ? 
_em_3d_reconstruction.actual_pixel_size           ? 
_em_3d_reconstruction.magnification_calibration   ? 
# 
_em_buffer.id            1 
_em_buffer.details       ? 
_em_buffer.pH            8 
_em_buffer.specimen_id   1 
_em_buffer.name          ? 
# 
_em_entity_assembly.id                   1 
_em_entity_assembly.parent_id            0 
_em_entity_assembly.details              'Dimeric complex' 
_em_entity_assembly.go_id                ? 
_em_entity_assembly.name                 'Human Coronavirus HKU1 Haemagglutinin-Esterase' 
_em_entity_assembly.source               RECOMBINANT 
_em_entity_assembly.type                 COMPLEX 
_em_entity_assembly.entity_id_list       1 
_em_entity_assembly.ipr_id               ? 
_em_entity_assembly.synonym              ? 
_em_entity_assembly.number_of_copies     ? 
_em_entity_assembly.oligomeric_details   ? 
# 
_em_image_scans.entry_id                EMD-10676 
_em_image_scans.id                      1 
_em_image_scans.dimension_height        4096 
_em_image_scans.dimension_width         4096 
_em_image_scans.frames_per_image        ? 
_em_image_scans.image_recording_id      1 
_em_image_scans.sampling_size           14 
_em_image_scans.scanner_model           ? 
_em_image_scans.used_frames_per_image   ? 
_em_image_scans.citation_id             ? 
_em_image_scans.number_digital_images   ? 
_em_image_scans.od_range                ? 
_em_image_scans.quant_bit_size          ? 
_em_image_scans.details                 ? 
# 
_em_imaging.id                              1 
_em_imaging.entry_id                        EMD-10676 
_em_imaging.accelerating_voltage            300 
_em_imaging.alignment_procedure             'COMA FREE' 
_em_imaging.c2_aperture_diameter            50 
_em_imaging.calibrated_defocus_max          ? 
_em_imaging.calibrated_defocus_min          ? 
_em_imaging.calibrated_magnification        ? 
_em_imaging.cryogen                         NITROGEN 
_em_imaging.details                         'Titan Krios G4 was used with fringe-free imaging and aberration-free image shift.' 
_em_imaging.electron_source                 'FIELD EMISSION GUN' 
_em_imaging.illumination_mode               'FLOOD BEAM' 
_em_imaging.microscope_model                'TFS KRIOS' 
_em_imaging.mode                            'BRIGHT FIELD' 
_em_imaging.nominal_cs                      2.7 
_em_imaging.nominal_defocus_max             -2500 
_em_imaging.nominal_defocus_min             -1000 
_em_imaging.nominal_magnification           96000 
_em_imaging.recording_temperature_maximum   ? 
_em_imaging.recording_temperature_minimum   ? 
_em_imaging.residual_tilt                   ? 
_em_imaging.specimen_holder_model           'FEI TITAN KRIOS AUTOGRID HOLDER' 
_em_imaging.specimen_id                     1 
_em_imaging.sample_support_id               1 
_em_imaging.citation_id                     ? 
_em_imaging.detector_id                     1 
_em_imaging.scans_id                        1 
_em_imaging.date                            ? 
_em_imaging.temperature                     ? 
_em_imaging.tilt_angle_min                  ? 
_em_imaging.tilt_angle_max                  ? 
_em_imaging.astigmatism                     ? 
_em_imaging.detector_distance               ? 
_em_imaging.electron_beam_tilt_params       ? 
_em_imaging.specimen_holder_type            ? 
# 
_em_sample_support.id               1 
_em_sample_support.specimen_id      1 
_em_sample_support.details          '20 mA' 
_em_sample_support.grid_material    COPPER 
_em_sample_support.grid_mesh_size   200 
_em_sample_support.grid_type        'Quantifoil R1.2/1.3' 
_em_sample_support.method           ? 
_em_sample_support.film_material    ? 
# 
_em_vitrification.id                      1 
_em_vitrification.specimen_id             1 
_em_vitrification.chamber_temperature     277 
_em_vitrification.cryogen_name            ETHANE 
_em_vitrification.details                 
;Blot force = 1
Blot time = 5.5 seconds
;
_em_vitrification.humidity                95 
_em_vitrification.instrument              'FEI VITROBOT MARK IV' 
_em_vitrification.entry_id                EMD-10676 
_em_vitrification.sample_preparation_id   ? 
_em_vitrification.citation_id             ? 
_em_vitrification.method                  ? 
_em_vitrification.temp                    ? 
_em_vitrification.time_resolved_state     ? 
# 
_em_experiment.entry_id                EMD-10676 
_em_experiment.id                      1 
_em_experiment.aggregation_state       PARTICLE 
_em_experiment.reconstruction_method   'SINGLE PARTICLE' 
_em_experiment.entity_assembly_id      1 
# 
_em_single_particle_entity.entry_id              EMD-10676 
_em_single_particle_entity.id                    1 
_em_single_particle_entity.image_processing_id   1 
_em_single_particle_entity.point_symmetry        C2 
# 
_em_admin.entry_id              EMD-10676 
_em_admin.current_status        REL 
_em_admin.deposition_date       2020-02-19 
_em_admin.deposition_site       PDBE 
_em_admin.details               ? 
_em_admin.last_update           2024-11-20 
_em_admin.map_release_date      2020-04-08 
_em_admin.obsoleted_date        ? 
_em_admin.title                 'Human Coronavirus HKU1 Haemagglutinin-Esterase' 
_em_admin.header_release_date   2020-04-08 
# 
loop_
_em_author_list.ordinal 
_em_author_list.author 
1 'Hurdiss, D.L.' 
2 'Drulyte, I.'   
# 
loop_
_em_buffer_component.buffer_id 
_em_buffer_component.id 
_em_buffer_component.concentration 
_em_buffer_component.concentration_units 
_em_buffer_component.formula 
_em_buffer_component.name 
1 1 10 mM C4H11NO3 Trisaminomethane  
1 2 50 mM NaCl     'Sodium chloride' 
# 
_em_ctf_correction.id                                1 
_em_ctf_correction.em_image_processing_id            1 
_em_ctf_correction.type                              'PHASE FLIPPING AND AMPLITUDE CORRECTION' 
_em_ctf_correction.details                           ? 
_em_ctf_correction.amplitude_correction              ? 
_em_ctf_correction.amplitude_correction_factor       ? 
_em_ctf_correction.amplitude_correction_space        ? 
_em_ctf_correction.correction_operation              ? 
_em_ctf_correction.phase_reversal                    ? 
_em_ctf_correction.phase_reversal_anisotropic        ? 
_em_ctf_correction.phase_reversal_correction_space   ? 
# 
_em_db_reference.id             1 
_em_db_reference.access_code    6Y3Y 
_em_db_reference.db_name        PDB 
_em_db_reference.details        ? 
_em_db_reference.relationship   'IN FRAME' 
# 
loop_
_em_entity_assembly_molwt.entity_assembly_id 
_em_entity_assembly_molwt.id 
_em_entity_assembly_molwt.experimental_flag 
_em_entity_assembly_molwt.units 
_em_entity_assembly_molwt.value 
1 1 NO  MEGADALTONS 0.080784 
1 2 YES MEGADALTONS 0.030777 
# 
_em_entity_assembly_naturalsource.id                   2 
_em_entity_assembly_naturalsource.entity_assembly_id   1 
_em_entity_assembly_naturalsource.cell                 ? 
_em_entity_assembly_naturalsource.cellular_location    ? 
_em_entity_assembly_naturalsource.ncbi_tax_id          290028 
_em_entity_assembly_naturalsource.organ                ? 
_em_entity_assembly_naturalsource.organelle            ? 
_em_entity_assembly_naturalsource.organism             'Human coronavirus HKU1' 
_em_entity_assembly_naturalsource.strain               ? 
_em_entity_assembly_naturalsource.tissue               ? 
# 
_em_entity_assembly_recombinant.id                   2 
_em_entity_assembly_recombinant.entity_assembly_id   1 
_em_entity_assembly_recombinant.cell                 ? 
_em_entity_assembly_recombinant.ncbi_tax_id          9606 
_em_entity_assembly_recombinant.organism             'Homo sapiens' 
_em_entity_assembly_recombinant.plasmid              ? 
_em_entity_assembly_recombinant.strain               ? 
# 
loop_
_em_euler_angle_assignment.id 
_em_euler_angle_assignment.details 
_em_euler_angle_assignment.image_processing_id 
_em_euler_angle_assignment.order 
_em_euler_angle_assignment.proj_matching_angular_sampling 
_em_euler_angle_assignment.proj_matching_merit_function 
_em_euler_angle_assignment.proj_matching_num_projections 
_em_euler_angle_assignment.type 
1 ? 1 INITIAL ? ? ? 'NOT APPLICABLE' 
2 ? 1 FINAL   ? ? ? 'NOT APPLICABLE' 
# 
_em_final_classification.id                         1 
_em_final_classification.type                       3D 
_em_final_classification.avg_num_images_per_class   141000 
_em_final_classification.details                    ? 
_em_final_classification.image_processing_id        1 
_em_final_classification.num_classes                4 
# 
_em_grid_pretreatment.id                  1 
_em_grid_pretreatment.sample_support_id   1 
_em_grid_pretreatment.atmosphere          Air 
_em_grid_pretreatment.pressure            ? 
_em_grid_pretreatment.time                30 
_em_grid_pretreatment.type                'GLOW DISCHARGE' 
# 
_em_image_processing.id                   1 
_em_image_processing.image_recording_id   1 
_em_image_processing.details              'Falcon 4 Direct Electron Detector' 
# 
_em_image_recording.id                            1 
_em_image_recording.imaging_id                    1 
_em_image_recording.avg_electron_dose_per_image   40 
_em_image_recording.average_exposure_time         5.6 
_em_image_recording.details                       'Falcon 4 Direct Electron Detector' 
_em_image_recording.detector_mode                 ? 
_em_image_recording.film_or_detector_model        OTHER 
_em_image_recording.num_diffraction_images        ? 
_em_image_recording.num_grids_imaged              1 
_em_image_recording.num_real_images               6029 
# 
loop_
_em_map.entry_id 
_em_map.id 
_em_map.annotation_details 
_em_map.axis_order_fast 
_em_map.axis_order_medium 
_em_map.axis_order_slow 
_em_map.cell_a 
_em_map.cell_alpha 
_em_map.cell_b 
_em_map.cell_beta 
_em_map.cell_c 
_em_map.cell_gamma 
_em_map.contour_level 
_em_map.contour_level_source 
_em_map.data_type 
_em_map.dimensions_col 
_em_map.dimensions_row 
_em_map.dimensions_sec 
_em_map.endian_type 
_em_map.file 
_em_map.format 
_em_map.label 
_em_map.limit_col 
_em_map.limit_row 
_em_map.limit_sec 
_em_map.origin_col 
_em_map.origin_row 
_em_map.origin_sec 
_em_map.partition 
_em_map.pixel_spacing_x 
_em_map.pixel_spacing_y 
_em_map.pixel_spacing_z 
_em_map.size_kb 
_em_map.spacing_x 
_em_map.spacing_y 
_em_map.spacing_z 
_em_map.statistics_average 
_em_map.statistics_maximum 
_em_map.statistics_minimum 
_em_map.statistics_std 
_em_map.symmetry_space_group 
_em_map.type 
EMD-10676 1 'Human coronavirus-HKU1 haemagglutinin esterase (local resolution filtered map).'             X Y Z 257.6 90.0 257.6 
90.0 257.6 90.0 0.0601  author 'Image stored as floating point number (4 bytes)' 320 320 320 big emd_10676.map.gz              
CCP4 ::::EMDATABANK.org::::EMD-10676:::: 319 319 319 0 0 0 1 0.805 0.805 0.805 131073024 320 320 320 -5.267036E-5   0.29912442  
-0.17708412   0.005566763  1 'primary map'    
EMD-10676 2 'Human coronavirus-HKU1 haemagglutinin esterase (Gaussian filtered N-glycan difference map).' X Y Z 257.6 90.0 257.6 
90.0 257.6 90.0 0.00128 author 'Image stored as floating point number (4 bytes)' 320 320 320 big emd_10676_additional_1.map.gz 
CCP4 ::::EMDATABANK.org::::EMD-10676:::: 319 319 319 0 0 0 1 0.805 0.805 0.805 131073024 320 320 320 -2.9664618E-4  0.021840053 
-0.0072245575 8.013045E-4  1 'additional map' 
EMD-10676 3 'Human coronavirus-HKU1 haemagglutinin esterase (unsharpened map).'                           X Y Z 257.6 90.0 257.6 
90.0 257.6 90.0 0.0192  author 'Image stored as floating point number (4 bytes)' 320 320 320 big emd_10676_additional_2.map.gz 
CCP4 ::::EMDATABANK.org::::EMD-10676:::: 319 319 319 0 0 0 2 0.805 0.805 0.805 131073024 320 320 320 -3.0976714e-06 0.05665717  
-0.022491328  0.0017983388 1 'additional map' 
EMD-10676 4 'Human coronavirus-HKU1 haemagglutinin esterase (sharpened map).'                             X Y Z 257.6 90.0 257.6 
90.0 257.6 90.0 0.0344  author 'Image stored as floating point number (4 bytes)' 320 320 320 big emd_10676_additional_3.map.gz 
CCP4 ::::EMDATABANK.org::::EMD-10676:::: 319 319 319 0 0 0 3 0.805 0.805 0.805 131073024 320 320 320 0.00013442665  0.15049002  
-0.08593951   0.0028335769 1 'additional map' 
EMD-10676 7 'Mask used during post-processing.'                                                           X Y Z 257.6 90.0 257.6 
90.0 257.6 90.0 0.123   author 'Image stored as floating point number (4 bytes)' 320 320 320 big emd_10676_msk_1.map           
CCP4 ::::EMDATABANK.org::::EMD-10676:::: 319 319 319 0 0 0 1 0.805 0.805 0.805 131073024 320 320 320 0.014501168    1.0         
0.0           0.11580913   1 mask             
EMD-10676 8 'Human coronavirus-HKU1 haemagglutinin esterase (half map 2).'                                X Y Z 257.6 90.0 257.6 
90.0 257.6 90.0 0.0177  author 'Image stored as floating point number (4 bytes)' 320 320 320 big emd_10676_half_map_1.map.gz   
CCP4 ::::EMDATABANK.org::::EMD-10676:::: 319 319 319 0 0 0 1 0.805 0.805 0.805 131073024 320 320 320 -3.0167676E-6  0.06399179  
-0.02744032   0.002102144  1 'half map'       
EMD-10676 9 'Human coronavirus-HKU1 haemagglutinin esterase (half map 1).'                                X Y Z 257.6 90.0 257.6 
90.0 257.6 90.0 0.0177  author 'Image stored as floating point number (4 bytes)' 320 320 320 big emd_10676_half_map_2.map.gz   
CCP4 ::::EMDATABANK.org::::EMD-10676:::: 319 319 319 0 0 0 2 0.805 0.805 0.805 131073024 320 320 320 -3.8577537e-06 0.06319329  
-0.02807623   0.0020963992 1 'half map'       
# 
_em_particle_selection.id                       1 
_em_particle_selection.image_processing_id      1 
_em_particle_selection.details                  'Relion autopicking' 
_em_particle_selection.method                   ? 
_em_particle_selection.num_particles_selected   936260 
_em_particle_selection.reference_model          ? 
# 
loop_
_em_software.id 
_em_software.category 
_em_software.details 
_em_software.name 
_em_software.version 
_em_software.image_processing_id 
_em_software.fitting_id 
_em_software.imaging_id 
1  'PARTICLE SELECTION'       ? RELION         3.0.1 1 ? ? 
2  'IMAGE ACQUISITION'        ? EPU            2.5   ? ? 1 
3  MASKING                    ? ?              ?     ? ? ? 
4  'CTF CORRECTION'           ? Gctf           1.18  1 ? ? 
5  'LAYERLINE INDEXING'       ? ?              ?     ? ? ? 
6  'DIFFRACTION INDEXING'     ? ?              ?     ? ? ? 
7  'MODEL FITTING'            ? 'UCSF Chimera' 1.12  ? 1 ? 
8  OTHER                      ? ?              ?     ? ? ? 
9  'INITIAL EULER ASSIGNMENT' ? RELION         3.1   1 ? ? 
10 'FINAL EULER ASSIGNMENT'   ? RELION         3.1   1 ? ? 
11 CLASSIFICATION             ? RELION         3.0.1 1 ? ? 
12 RECONSTRUCTION             ? RELION         3.1   1 ? ? 
13 'MODEL REFINEMENT'         ? PHENIX         1.13  ? 1 ? 
# 
_em_specimen.id                      1 
_em_specimen.experiment_id           1 
_em_specimen.concentration           4 
_em_specimen.details                 ? 
_em_specimen.embedding_applied       NO 
_em_specimen.shadowing_applied       NO 
_em_specimen.staining_applied        NO 
_em_specimen.vitrification_applied   YES 
# 
_em_start_model.id                               1 
_em_start_model.details                          ? 
_em_start_model.image_processing_id              1 
_em_start_model.emdb_id                          ? 
_em_start_model.insilico_model                   
;A homology model of the dimeric HCoV-HKU1 HE (uniprot ID: Q5MQD1) was generated using the phyre2 server. The molmap command in UCSF chimera, was then used to create a 40 angstrom resolution starting model.
;
_em_start_model.orthogonal_tilt_angle1           ? 
_em_start_model.orthogonal_tilt_angle2           ? 
_em_start_model.orthogonal_tilt_num_images       ? 
_em_start_model.other                            ? 
_em_start_model.pdb_id                           ? 
_em_start_model.random_conical_tilt_angle        ? 
_em_start_model.random_conical_tilt_num_images   ? 
_em_start_model.type                             'INSILICO MODEL' 
# 
_em_support_film.id                  1 
_em_support_film.sample_support_id   1 
_em_support_film.material            CARBON 
_em_support_film.thickness           ? 
_em_support_film.topology            HOLEY 
# 
