data_EMD-10564
# 
_entry.id   EMD-10564 
# 
_audit_conform.dict_name       mmcif_pdbx.dic 
_audit_conform.dict_version    5.392 
_audit_conform.dict_location   http://mmcif.pdb.org/dictionaries/ascii/mmcif_pdbx.dic 
# 
loop_
_database_2.database_id 
_database_2.database_code 
_database_2.pdbx_database_accession 
_database_2.pdbx_DOI 
PDB   6TSK         pdb_00006tsk 10.2210/pdb6tsk/pdb 
WWPDB D_1292106005 ?            ?                   
EMDB  EMD-10564    ?            ?                   
# 
loop_
_pdbx_database_related.db_name 
_pdbx_database_related.details 
_pdbx_database_related.db_id 
_pdbx_database_related.content_type 
EMDB 'Bgal in complex with 1-deoxygalacto-nojirimycin' EMD-10563 'other EM volume'      
EMDB 'Beta-galactosidase in complex with L-ribose'     EMD-10564 'associated EM volume' 
# 
_citation.abstract                  ? 
_citation.abstract_id_CAS           ? 
_citation.book_id_ISBN              ? 
_citation.book_publisher            ? 
_citation.book_publisher_city       ? 
_citation.book_title                ? 
_citation.coordinate_linkage        ? 
_citation.country                   UK 
_citation.database_id_Medline       ? 
_citation.details                   ? 
_citation.id                        primary 
_citation.journal_abbrev            'Drug Discov Today' 
_citation.journal_id_ASTM           ? 
_citation.journal_id_CSD            ? 
_citation.journal_id_ISSN           1878-5832 
_citation.journal_full              ? 
_citation.journal_issue             ? 
_citation.journal_volume            25 
_citation.language                  ? 
_citation.page_first                485 
_citation.page_last                 490 
_citation.title                     'Fragment-based drug discovery using cryo-EM.' 
_citation.year                      2020 
_citation.database_id_CSD           ? 
_citation.pdbx_database_id_DOI      10.1016/j.drudis.2019.12.006 
_citation.pdbx_database_id_PubMed   31877353 
_citation.unpublished_flag          ? 
# 
loop_
_citation_author.citation_id 
_citation_author.name 
_citation_author.ordinal 
_citation_author.identifier_ORCID 
primary 'Saur, M.'        1 ? 
primary 'Hartshorn, M.J.' 2 ? 
primary 'Dong, J.'        3 ? 
primary 'Reeks, J.'       4 ? 
primary 'Bunkoczi, G.'    5 ? 
primary 'Jhoti, H.'       6 ? 
primary 'Williams, P.A.'  7 ? 
# 
loop_
_entity.id 
_entity.type 
_entity.src_method 
_entity.pdbx_description 
_entity.formula_weight 
_entity.pdbx_number_of_molecules 
_entity.pdbx_ec 
_entity.pdbx_mutation 
_entity.pdbx_fragment 
_entity.details 
1 polymer     man Beta-galactosidase  118395.336 4   3.2.1.23 ? ? ? 
2 non-polymer syn 'MAGNESIUM ION'     24.305     4   ?        ? ? ? 
3 non-polymer syn beta-L-ribopyranose 150.130    4   ?        ? ? ? 
4 water       nat water               18.015     964 ?        ? ? ? 
# 
_entity_poly.entity_id                  1 
_entity_poly.type                       'polypeptide(L)' 
_entity_poly.pdbx_seq_one_letter_code   
;MGSSHHHHHHSSGLVPRGSHMLEDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWF
PAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIF
DGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQIS
DFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA
EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNN
FNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESG
HGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMG
NSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQP
NATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQ
FTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQ
MAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRE
LNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK
;
# 
loop_
_pdbx_entity_nonpoly.entity_id 
_pdbx_entity_nonpoly.name 
_pdbx_entity_nonpoly.comp_id 
2 'MAGNESIUM ION'     MG  
3 beta-L-ribopyranose 0MK 
4 water               HOH 
# 
_entity_src_gen.entity_id                          1 
_entity_src_gen.pdbx_src_id                        1 
_entity_src_gen.pdbx_alt_source_flag               sample 
_entity_src_gen.pdbx_seq_type                      'Biological sequence' 
_entity_src_gen.pdbx_beg_seq_num                   1 
_entity_src_gen.pdbx_end_seq_num                   1040 
_entity_src_gen.gene_src_common_name               ? 
_entity_src_gen.gene_src_genus                     ? 
_entity_src_gen.pdbx_gene_src_gene                 lacZ 
_entity_src_gen.gene_src_species                   ? 
_entity_src_gen.gene_src_strain                    ? 
_entity_src_gen.gene_src_tissue                    ? 
_entity_src_gen.gene_src_tissue_fraction           ? 
_entity_src_gen.gene_src_details                   ? 
_entity_src_gen.pdbx_gene_src_fragment             ? 
_entity_src_gen.pdbx_gene_src_scientific_name      'Escherichia coli' 
_entity_src_gen.pdbx_gene_src_ncbi_taxonomy_id     562 
_entity_src_gen.pdbx_gene_src_variant              ? 
_entity_src_gen.pdbx_gene_src_cell_line            ? 
_entity_src_gen.pdbx_gene_src_atcc                 ? 
_entity_src_gen.pdbx_gene_src_culture_collection   ? 
_entity_src_gen.pdbx_gene_src_organ                ? 
_entity_src_gen.pdbx_gene_src_organelle            ? 
_entity_src_gen.pdbx_gene_src_cell                 ? 
_entity_src_gen.pdbx_gene_src_cellular_location    ? 
_entity_src_gen.host_org_common_name               ? 
_entity_src_gen.pdbx_host_org_scientific_name      'Escherichia coli' 
_entity_src_gen.pdbx_host_org_ncbi_taxonomy_id     562 
_entity_src_gen.host_org_genus                     ? 
_entity_src_gen.pdbx_host_org_gene                 ? 
_entity_src_gen.pdbx_host_org_organ                ? 
_entity_src_gen.host_org_species                   ? 
_entity_src_gen.pdbx_host_org_tissue               ? 
_entity_src_gen.pdbx_host_org_tissue_fraction      ? 
_entity_src_gen.pdbx_host_org_strain               ? 
_entity_src_gen.pdbx_host_org_variant              ? 
_entity_src_gen.pdbx_host_org_cell_line            ? 
_entity_src_gen.pdbx_host_org_atcc                 ? 
_entity_src_gen.pdbx_host_org_culture_collection   ? 
_entity_src_gen.pdbx_host_org_cell                 ? 
_entity_src_gen.pdbx_host_org_organelle            ? 
_entity_src_gen.pdbx_host_org_cellular_location    ? 
_entity_src_gen.pdbx_host_org_vector_type          plasmid 
_entity_src_gen.pdbx_host_org_vector               ? 
_entity_src_gen.host_org_details                   ? 
_entity_src_gen.expression_system_id               ? 
_entity_src_gen.plasmid_name                       pET-15b 
_entity_src_gen.plasmid_details                    ? 
_entity_src_gen.pdbx_description                   ? 
# 
loop_
_chem_comp.id 
_chem_comp.type 
_chem_comp.mon_nstd_flag 
_chem_comp.name 
_chem_comp.pdbx_synonyms 
_chem_comp.formula 
_chem_comp.formula_weight 
0MK 'L-saccharide, beta linking' . beta-L-ribopyranose 'beta-L-ribose; L-ribose; ribose; L-ribopyranose' 'C5 H10 O5'      150.130 
ALA 'L-peptide linking'          y ALANINE             ?                                                 'C3 H7 N O2'     89.093  
ARG 'L-peptide linking'          y ARGININE            ?                                                 'C6 H15 N4 O2 1' 175.209 
ASN 'L-peptide linking'          y ASPARAGINE          ?                                                 'C4 H8 N2 O3'    132.118 
ASP 'L-peptide linking'          y 'ASPARTIC ACID'     ?                                                 'C4 H7 N O4'     133.103 
CYS 'L-peptide linking'          y CYSTEINE            ?                                                 'C3 H7 N O2 S'   121.158 
GLN 'L-peptide linking'          y GLUTAMINE           ?                                                 'C5 H10 N2 O3'   146.144 
GLU 'L-peptide linking'          y 'GLUTAMIC ACID'     ?                                                 'C5 H9 N O4'     147.129 
GLY 'peptide linking'            y GLYCINE             ?                                                 'C2 H5 N O2'     75.067  
HIS 'L-peptide linking'          y HISTIDINE           ?                                                 'C6 H10 N3 O2 1' 156.162 
HOH non-polymer                  . WATER               ?                                                 'H2 O'           18.015  
ILE 'L-peptide linking'          y ISOLEUCINE          ?                                                 'C6 H13 N O2'    131.173 
LEU 'L-peptide linking'          y LEUCINE             ?                                                 'C6 H13 N O2'    131.173 
LYS 'L-peptide linking'          y LYSINE              ?                                                 'C6 H15 N2 O2 1' 147.195 
MET 'L-peptide linking'          y METHIONINE          ?                                                 'C5 H11 N O2 S'  149.211 
MG  non-polymer                  . 'MAGNESIUM ION'     ?                                                 'Mg 2'           24.305  
PHE 'L-peptide linking'          y PHENYLALANINE       ?                                                 'C9 H11 N O2'    165.189 
PRO 'L-peptide linking'          y PROLINE             ?                                                 'C5 H9 N O2'     115.130 
SER 'L-peptide linking'          y SERINE              ?                                                 'C3 H7 N O3'     105.093 
THR 'L-peptide linking'          y THREONINE           ?                                                 'C4 H9 N O3'     119.119 
TRP 'L-peptide linking'          y TRYPTOPHAN          ?                                                 'C11 H12 N2 O2'  204.225 
TYR 'L-peptide linking'          y TYROSINE            ?                                                 'C9 H11 N O3'    181.189 
VAL 'L-peptide linking'          y VALINE              ?                                                 'C5 H11 N O2'    117.146 
# 
_exptl.entry_id         EMD-10564 
_exptl.details          ? 
_exptl.method           'ELECTRON MICROSCOPY' 
_exptl.method_details   ? 
# 
_struct_keywords.entry_id        EMD-10564 
_struct_keywords.text            'Bgal, L-ribose, SUGAR BINDING PROTEIN' 
_struct_keywords.pdbx_keywords   'SUGAR BINDING PROTEIN' 
_struct_keywords.pdbx_details    ? 
# 
_struct_ref.id          1 
_struct_ref.db_name     UNP 
_struct_ref.db_code     BGAL_ECOLX 
_struct_ref.entity_id   1 
# 
_em_3d_fitting.entry_id          EMD-10564 
_em_3d_fitting.id                1 
_em_3d_fitting.details           ? 
_em_3d_fitting.overall_b_value   ? 
_em_3d_fitting.ref_protocol      ? 
_em_3d_fitting.ref_space         ? 
_em_3d_fitting.target_criteria   ? 
_em_3d_fitting.method            ? 
# 
_em_3d_reconstruction.entry_id                    EMD-10564 
_em_3d_reconstruction.id                          1 
_em_3d_reconstruction.algorithm                   ? 
_em_3d_reconstruction.details                     ? 
_em_3d_reconstruction.fsc_type                    ? 
_em_3d_reconstruction.refinement_type             ? 
_em_3d_reconstruction.image_processing_id         1 
_em_3d_reconstruction.num_class_averages          ? 
_em_3d_reconstruction.num_particles               40086 
_em_3d_reconstruction.resolution                  2.3 
_em_3d_reconstruction.resolution_method           'FSC 0.143 CUT-OFF' 
_em_3d_reconstruction.symmetry_type               POINT 
_em_3d_reconstruction.method                      ? 
_em_3d_reconstruction.nominal_pixel_size          ? 
_em_3d_reconstruction.actual_pixel_size           ? 
_em_3d_reconstruction.magnification_calibration   ? 
# 
_em_buffer.id            1 
_em_buffer.details       ? 
_em_buffer.pH            6.8 
_em_buffer.specimen_id   1 
_em_buffer.name          ? 
# 
_em_entity_assembly.id                   1 
_em_entity_assembly.parent_id            0 
_em_entity_assembly.details              ? 
_em_entity_assembly.go_id                ? 
_em_entity_assembly.name                 Beta-galactosidase 
_em_entity_assembly.source               RECOMBINANT 
_em_entity_assembly.type                 COMPLEX 
_em_entity_assembly.entity_id_list       1 
_em_entity_assembly.ipr_id               ? 
_em_entity_assembly.synonym              ? 
_em_entity_assembly.number_of_copies     ? 
_em_entity_assembly.oligomeric_details   ? 
# 
_em_imaging.id                              1 
_em_imaging.entry_id                        EMD-10564 
_em_imaging.accelerating_voltage            300 
_em_imaging.alignment_procedure             ? 
_em_imaging.c2_aperture_diameter            ? 
_em_imaging.calibrated_defocus_max          ? 
_em_imaging.calibrated_defocus_min          ? 
_em_imaging.calibrated_magnification        ? 
_em_imaging.cryogen                         ? 
_em_imaging.details                         ? 
_em_imaging.electron_source                 'FIELD EMISSION GUN' 
_em_imaging.illumination_mode               'FLOOD BEAM' 
_em_imaging.microscope_model                'FEI TITAN KRIOS' 
_em_imaging.mode                            'BRIGHT FIELD' 
_em_imaging.nominal_cs                      ? 
_em_imaging.nominal_defocus_max             ? 
_em_imaging.nominal_defocus_min             ? 
_em_imaging.nominal_magnification           ? 
_em_imaging.recording_temperature_maximum   ? 
_em_imaging.recording_temperature_minimum   ? 
_em_imaging.residual_tilt                   ? 
_em_imaging.specimen_holder_model           ? 
_em_imaging.specimen_id                     1 
_em_imaging.sample_support_id               1 
_em_imaging.citation_id                     ? 
_em_imaging.detector_id                     1 
_em_imaging.scans_id                        1 
_em_imaging.date                            ? 
_em_imaging.temperature                     ? 
_em_imaging.tilt_angle_min                  ? 
_em_imaging.tilt_angle_max                  ? 
_em_imaging.astigmatism                     ? 
_em_imaging.detector_distance               ? 
_em_imaging.electron_beam_tilt_params       ? 
_em_imaging.specimen_holder_type            ? 
# 
_em_sample_support.id               1 
_em_sample_support.specimen_id      1 
_em_sample_support.method           ? 
_em_sample_support.film_material    ? 
_em_sample_support.grid_material    ? 
_em_sample_support.grid_mesh_size   ? 
_em_sample_support.grid_type        ? 
_em_sample_support.details          ? 
# 
_em_vitrification.id                      1 
_em_vitrification.specimen_id             1 
_em_vitrification.chamber_temperature     277 
_em_vitrification.cryogen_name            ETHANE 
_em_vitrification.details                 ? 
_em_vitrification.humidity                100 
_em_vitrification.instrument              'FEI VITROBOT MARK IV' 
_em_vitrification.entry_id                EMD-10564 
_em_vitrification.sample_preparation_id   ? 
_em_vitrification.citation_id             ? 
_em_vitrification.method                  ? 
_em_vitrification.temp                    ? 
_em_vitrification.time_resolved_state     ? 
# 
_em_experiment.entry_id                EMD-10564 
_em_experiment.id                      1 
_em_experiment.aggregation_state       PARTICLE 
_em_experiment.reconstruction_method   'SINGLE PARTICLE' 
_em_experiment.entity_assembly_id      1 
# 
_em_single_particle_entity.entry_id              EMD-10564 
_em_single_particle_entity.id                    1 
_em_single_particle_entity.image_processing_id   1 
_em_single_particle_entity.point_symmetry        D2 
# 
_em_admin.entry_id              EMD-10564 
_em_admin.current_status        REL 
_em_admin.deposition_date       2019-12-20 
_em_admin.deposition_site       PDBE 
_em_admin.details               ? 
_em_admin.last_update           2024-05-22 
_em_admin.map_release_date      2020-01-08 
_em_admin.obsoleted_date        ? 
_em_admin.title                 'Beta-galactosidase in complex with L-ribose' 
_em_admin.header_release_date   2020-01-08 
# 
loop_
_em_author_list.ordinal 
_em_author_list.author 
1 'Saur, M.'        
2 'Hartshorn, M.J.' 
# 
_em_ctf_correction.id                                1 
_em_ctf_correction.em_image_processing_id            1 
_em_ctf_correction.type                              'PHASE FLIPPING AND AMPLITUDE CORRECTION' 
_em_ctf_correction.details                           ? 
_em_ctf_correction.amplitude_correction              ? 
_em_ctf_correction.amplitude_correction_factor       ? 
_em_ctf_correction.amplitude_correction_space        ? 
_em_ctf_correction.correction_operation              ? 
_em_ctf_correction.phase_reversal                    ? 
_em_ctf_correction.phase_reversal_anisotropic        ? 
_em_ctf_correction.phase_reversal_correction_space   ? 
# 
_em_db_reference.id             1 
_em_db_reference.access_code    6TSK 
_em_db_reference.db_name        PDB 
_em_db_reference.details        ? 
_em_db_reference.relationship   'IN FRAME' 
# 
_em_entity_assembly_molwt.entity_assembly_id   1 
_em_entity_assembly_molwt.id                   1 
_em_entity_assembly_molwt.experimental_flag    NO 
_em_entity_assembly_molwt.units                MEGADALTONS 
_em_entity_assembly_molwt.value                0.464 
# 
_em_entity_assembly_naturalsource.id                   1 
_em_entity_assembly_naturalsource.entity_assembly_id   1 
_em_entity_assembly_naturalsource.cell                 ? 
_em_entity_assembly_naturalsource.cellular_location    ? 
_em_entity_assembly_naturalsource.ncbi_tax_id          946522 
_em_entity_assembly_naturalsource.organ                ? 
_em_entity_assembly_naturalsource.organelle            ? 
_em_entity_assembly_naturalsource.organism             'Human alphaherpesvirus 1 strain RH2' 
_em_entity_assembly_naturalsource.strain               ? 
_em_entity_assembly_naturalsource.tissue               ? 
# 
_em_entity_assembly_recombinant.id                   1 
_em_entity_assembly_recombinant.entity_assembly_id   1 
_em_entity_assembly_recombinant.cell                 ? 
_em_entity_assembly_recombinant.ncbi_tax_id          562 
_em_entity_assembly_recombinant.organism             'Escherichia coli' 
_em_entity_assembly_recombinant.plasmid              pET-15b 
_em_entity_assembly_recombinant.strain               ? 
# 
loop_
_em_euler_angle_assignment.id 
_em_euler_angle_assignment.details 
_em_euler_angle_assignment.image_processing_id 
_em_euler_angle_assignment.order 
_em_euler_angle_assignment.proj_matching_angular_sampling 
_em_euler_angle_assignment.proj_matching_merit_function 
_em_euler_angle_assignment.proj_matching_num_projections 
_em_euler_angle_assignment.type 
1 ? 1 INITIAL ? ? ? 'MAXIMUM LIKELIHOOD' 
2 ? 1 FINAL   ? ? ? 'MAXIMUM LIKELIHOOD' 
# 
_em_image_processing.id                   1 
_em_image_processing.image_recording_id   1 
_em_image_processing.details              ? 
# 
_em_image_recording.id                            1 
_em_image_recording.imaging_id                    1 
_em_image_recording.avg_electron_dose_per_image   66.83 
_em_image_recording.average_exposure_time         59.98 
_em_image_recording.details                       ? 
_em_image_recording.detector_mode                 COUNTING 
_em_image_recording.film_or_detector_model        'FEI FALCON III (4k x 4k)' 
_em_image_recording.num_diffraction_images        ? 
_em_image_recording.num_grids_imaged              1 
_em_image_recording.num_real_images               517 
# 
loop_
_em_map.entry_id 
_em_map.id 
_em_map.annotation_details 
_em_map.axis_order_fast 
_em_map.axis_order_medium 
_em_map.axis_order_slow 
_em_map.cell_a 
_em_map.cell_alpha 
_em_map.cell_b 
_em_map.cell_beta 
_em_map.cell_c 
_em_map.cell_gamma 
_em_map.contour_level 
_em_map.contour_level_source 
_em_map.data_type 
_em_map.dimensions_col 
_em_map.dimensions_row 
_em_map.dimensions_sec 
_em_map.endian_type 
_em_map.file 
_em_map.format 
_em_map.label 
_em_map.limit_col 
_em_map.limit_row 
_em_map.limit_sec 
_em_map.origin_col 
_em_map.origin_row 
_em_map.origin_sec 
_em_map.partition 
_em_map.pixel_spacing_x 
_em_map.pixel_spacing_y 
_em_map.pixel_spacing_z 
_em_map.size_kb 
_em_map.spacing_x 
_em_map.spacing_y 
_em_map.spacing_z 
_em_map.statistics_average 
_em_map.statistics_maximum 
_em_map.statistics_minimum 
_em_map.statistics_std 
_em_map.symmetry_space_group 
_em_map.type 
EMD-10564 1 None                               X Y Z 95.88  90.0 149.6  90.0 199.24 90.0 0.05  author 
'Image stored as floating point number (4 bytes)' 141 220 293 big emd_10564.map.gz              CCP4 
::::EMDATABANK.org::::EMD-10564:::: 140 219 292 0 0 0 1 0.68 0.68 0.68 36356464 141 220 293 0.0021588702   0.24212413 -0.14002374 
0.020375106  1 'primary map'    
EMD-10564 2 'Relion auto-refine half map 2'    X Y Z 300.56 90.0 300.56 90.0 300.56 90.0 0.025 author 
'Image stored as floating point number (4 bytes)' 442 442 442 big emd_10564_additional_1.map.gz CCP4 
::::EMDATABANK.org::::EMD-10564:::: 441 441 441 0 0 0 1 0.68 0.68 0.68 36356464 442 442 442 -1.0381083e-06 0.0955504  -0.04937765 
0.005254949  1 'additional map' 
EMD-10564 3 'Relion auto-refine half map 1'    X Y Z 300.56 90.0 300.56 90.0 300.56 90.0 0.025 author 
'Image stored as floating point number (4 bytes)' 442 442 442 big emd_10564_additional_2.map.gz CCP4 
::::EMDATABANK.org::::EMD-10564:::: 441 441 441 0 0 0 2 0.68 0.68 0.68 36356464 442 442 442 -1.907422e-06  0.09508833 -0.04710385 
0.0052378993 1 'additional map' 
EMD-10564 4 'Relion post-process unmasked map' X Y Z 300.56 90.0 300.56 90.0 300.56 90.0 0.05  author 
'Image stored as floating point number (4 bytes)' 442 442 442 big emd_10564_additional_3.map.gz CCP4 
::::EMDATABANK.org::::EMD-10564:::: 441 441 441 0 0 0 3 0.68 0.68 0.68 36356464 442 442 442 -1.4727651e-06 0.24212413 -0.14002374 
0.009792831  1 'additional map' 
# 
_em_particle_selection.id                       1 
_em_particle_selection.image_processing_id      1 
_em_particle_selection.details                  ? 
_em_particle_selection.method                   ? 
_em_particle_selection.num_particles_selected   124686 
_em_particle_selection.reference_model          ? 
# 
loop_
_em_software.id 
_em_software.category 
_em_software.details 
_em_software.name 
_em_software.version 
_em_software.image_processing_id 
_em_software.fitting_id 
_em_software.imaging_id 
1  'PARTICLE SELECTION'       ? ?      ?    1 ? ? 
2  'IMAGE ACQUISITION'        ? ?      ?    ? ? 1 
3  MASKING                    ? ?      ?    ? ? ? 
4  'CTF CORRECTION'           ? ?      ?    1 ? ? 
5  'LAYERLINE INDEXING'       ? ?      ?    ? ? ? 
6  'DIFFRACTION INDEXING'     ? ?      ?    ? ? ? 
7  'MODEL FITTING'            ? ?      ?    ? 1 ? 
8  OTHER                      ? ?      ?    ? ? ? 
9  'MODEL REFINEMENT'         ? ?      ?    ? 1 ? 
10 'INITIAL EULER ASSIGNMENT' ? ?      ?    1 ? ? 
11 'FINAL EULER ASSIGNMENT'   ? ?      ?    1 ? ? 
12 CLASSIFICATION             ? ?      ?    1 ? ? 
13 RECONSTRUCTION             ? RELION 3.04 1 ? ? 
# 
_em_specimen.id                      1 
_em_specimen.experiment_id           1 
_em_specimen.concentration           0.17 
_em_specimen.details                 ? 
_em_specimen.embedding_applied       NO 
_em_specimen.shadowing_applied       NO 
_em_specimen.staining_applied        NO 
_em_specimen.vitrification_applied   YES 
# 
_em_start_model.id                               1 
_em_start_model.details                          ? 
_em_start_model.image_processing_id              1 
_em_start_model.emdb_id                          EMD-4116 
_em_start_model.insilico_model                   ? 
_em_start_model.orthogonal_tilt_angle1           ? 
_em_start_model.orthogonal_tilt_angle2           ? 
_em_start_model.orthogonal_tilt_num_images       ? 
_em_start_model.other                            ? 
_em_start_model.pdb_id                           ? 
_em_start_model.random_conical_tilt_angle        ? 
_em_start_model.random_conical_tilt_num_images   ? 
_em_start_model.type                             'EMDB MAP' 
# 
_em_support_film.id                  1 
_em_support_film.sample_support_id   1 
_em_support_film.material            'GRAPHENE OXIDE' 
_em_support_film.thickness           ? 
_em_support_film.topology            CONTINUOUS 
# 
