2024-05-22 REL PDBe PDBe PDBe 2019-12-09 2019-12-18 2020-09-30 2024-05-22 French National Research Agency France Oerum S Dendooven T Mini-RNase III, Mini-III, complex, ribosome, 50S, RNA maturation, RNA processing, precursor 23S rRNA, pre-23S rRNA, 23S rRNA, L3, Ribosomal protein, RNase III-like fold, RNase Oerum S Dendooven T Catala M Gilet L Degut C Trinquier A Bourguet M Barraud P Cianferani S Luisi BF Condon C Tisne C Mol.Cell US 80 227 2020 32991829 doi:10.1016/j.molcel.2020.09.008 1097-2765 2168 MOCEFL 6tnn FULLOVERLAP Mini-RNase III bound to 50S ribosome with precursor 23S rRNA Mini-RNase III bound to 50S ribosome with precursor 23S rRNA 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Mini-RNase III bound to 50S ribosome with precursor 23S rRNA 1 1 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Bacillus subtilis subsp. subtilis str. 168 Mini-RNase III bound to 50S ribosome with precursor 23S rRNA 1 2 Bacillus subtilis subsp. subtilis str. 168 50S ribosomal protein L10 Bacillus subtilis subsp. subtilis str. 168 0.018101747999999997 1 LEVO MSSAIETKKVVVEEIASKLKESKSTIIVDYRGLNVSEVTELRKQLREANVEFKVYKNTMTRRAVEQAELNGLNDFLTGPN AIAFSTEDVVAPAKVLNDFAKNHEALEIKAGVIEGKVSTVEEVKALAELPSREGLLSMLLSVLQAPVRNLALAAKAVAEQ KEEQGA P42923 Mini-ribonuclease 3 Bacillus subtilis subsp. subtilis str. 168 0.016251370999999997 2 Escherichia coli LEVO MLEFDTIKDSKQLNGLALAYIGNAIFEVYVRHHLLKQGFTKPNDLHKKSSRIVSAKSQAEILFFLQNQSFFTEEEEAVLK RGRNAKSGTTPKNTDVQTYRYSTAFEALLGYLFLEKKEERLSQLVAEAIQFGTSGRKTNESAT O31418 3.1.26.- pre-23S rRNA Bacillus subtilis subsp. subtilis str. 168 0.950280812 1 ACGGUUAAGUUAGAAAGGGCGCACGGUGGAUGCCUUGGCACUAGGAGCCGAUGAAGGACGGGACGAACACCGAUAUGCUU CGGGGAGCUGUAAGCAAGCUUUGAUCCGGAGAUUUCCGAAUGGGGAAACCCACCACUCGUAAUGGAGUGGUAUCCAUAUC UGAAUUCAUAGGAUAUGAGAAGGCAGACCCGGGGAACUGAAACAUCUAAGUACCCGGAGGAAGAGAAAGCAAAUGCGAUU CCCUGAGUAGCGGCGAGCGAAACGGGAUCAGCCCAAACCAAGAGGCUUGCCUCUUGGGGUUGUAGGACACUCUGUACGGA GUUACAAAGGAACGAGGUAGAUGAAGAGGUCUGGAAAGGCCCGCCAUAGGAGGUAACAGCCCUGUAGUCAAAACUUCGUU CUCUCCUGAGUGGAUCCUGAGUACGGCGGAACACGUGAAAUUCCGUCGGAAUCCGGGAGGACCAUCUCCCAAGGCUAAAU ACUCCCUAGUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGUGAAAAGCACCCCGGAAGGGGAGUGAAAGAGAUCCUG AAACCGUGUGCCUACAAGUAGUCAGAGCCCGUUAACGGGUGAUGGCGUGCCUUUUGUAGAAUGAACCGGCGAGUUACGAU CCCGUGCAAGGUUAAGCAGAAGAUGCGGAGCCGCAGCGAAAGCGAGUCUGAAUAGGGCGCAUGAGUACGUGGUCGUAGAC CCGAAACCAGGUGAUCUACCCAUGUCCAGGGUGAAGUUCAGGUAACACUGAAUGGAGGCCCGAACCCACGCACGUUGAAA AGUGCGGGGAUGAGGUGUGGGUAGGGGUGAAAUGCCAAUCGAACCUGGAGAUAGCUGGUUCUCUCCGAAAUAGCUUUAGG GCUAGCCUCAAGGUAAGAGUCUUGGAGGUAGAGCACUGAUUGGACUAGGGGCCCCUACCGGGUUACCGAAUUCAGUCAAA CUCCGAAUGCCAAUGACUUAUCCUUGGGAGUCAGACUGCGAGUGAUAAGAUCCGUAGUCGAAAGGGAAACAGCCCAGACC GCCAGCUAAGGUCCCAAAGUAUACGUUAAGUGGAAAAGGAUGUGGAGUUGCUUAGACAACCAGGAUGUUGGCUUAGAAGC AGCCACCAUUUAAAGAGUGCGUAAUAGCUCACUGGUCGAGUGACUCUGCGCCGAAAAUGUACCGGGGCUAAACGUAUCAC CGAAGCUGCGGACUGUUCUUCGAACAGUGGUAGGAGAGCGUUCUAAGGGCUGUGAAGCCAGACCGGAAGGACUGGUGGAG CGCUUAGAAGUGAGAAUGCCGGUAUGAGUAGCGAAAGAGGGGUGAGAAUCCCCUCCACCGAAUGCCUAAGGUUUCCUGAG GAAGGCUCGUCCGCUCAGGGUUAGUCGGGACCUAAGCCGAGGCCGAAAGGCGUAGGCGAUGGACAACAGGUUGAUAUUCC UGUACCACCUCCUCACCAUUUGAGCAAUGGGGGGACGCAGGAGGAUAGGGUAAGCGCGGUAUUGGAUAUCCGCGUCCAAG CAGUUAGGCUGGGAAAUAGGCAAAUCCGUUUCCCAUAAGGCUGAGCUGUGAUGGCGAGCGAAAUAUAGUAGCGAAGUUCC UGAUUCCACACUGCCAAGAAAAGCCUCUAGCGAGGUGAGAGGUGCCCGUACCGCAAACCGACACAGGUAGGCGAGGAGAG AAUCCUAAGGUGAUCGAGAGAACUCUCGUUAAGGAACUCGGCAAAAUGACCCCGUAACUUCGGGAGAAGGGGUGCUCUGU UAGGGUGCAAGCCCGAGAGAGCCGCAGUGAAUAGGCCCAGGCGACUGUUUAGCAAAAACACAGGUCUCUGCGAAGCCGUA AGGCGAAGUAUAGGGGCUGACGCCUGCCCGGUGCUGGAAGGUUAAGAGGAGCGCUUAGCGUAAGCGAAGGUGCGAAUUGA AGCCCCAGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGA AAGGCGCAACGAUCUGGGCACUGUCUCAACGAGAGACUCGGUGAAAUUAUAGUACCUGUGAAGAUGCAGGUUACCCGCGA CAGGACGGAAAGACCCCGUGGAGCUUUACUGCAGCCUGAUAUUGAAUGUUGGUACAGCUUGUACAGGAUAGGUAGGAGCC UUGGAAACCGGAGCGCCAGCUUUGGUGGAGGCAUCGGUGGGAUACUACCCUGGCUGUAUUGACCUUCUAACCCGCCGCCC UUAUCGGGCGGGGAGACAGUGUCAGGUGGGCAGUUUGACUGGGGCGGUCGCCUCCUAAAAGGUAACGGAGGCGCCCAAAG GUUCCCUCAGAAUGGUUGGAAAUCAUUCGCAGAGUGUAAAGGCACAAGGGAGCUUGACUGCGAGACCUACAAGUCGAGCA GGGACGAAAGUCGGGCUUAGUGAUCCGGUGGUUCCGCAUGGAAGGGCCAUCGCUCAACGGAUAAAAGCUACCCCGGGGAU AACAGGCUUAUCUCCCCCAAGAGUCCACAUCGACGGGGAGGUUUGGCACCUCGAUGUCGGCUCAUCGCAUCCUGGGGCUG UAGUCGGUCCCAAGGGUUGGGCUGUUCGCCCAUUAAAGCGGUACGCGAGCUGGGUUCAGAACGUCGUGAGACAGUUCGGU CCCUAUCCGUCGCGGGCGCAGGAAAUUUGAGAGGAGCUGUCCUUAGUACGAGAGGACCGGGAUGGACGCACCGCUGGUGU ACCAGUUGUUCUGCCAAGGGCAUCGCUGGGUAGCUAUGUGCGGACGGGAUAAGUGCUGAAAGCAUCUAAGCAUGAAGCCC CCCUCAAGAUGAGAUUUCCCAUUCCGCAAGGAAGUAAGAUCCCUGAAAGAUGAUCAGGUUGAUAGGUCUGAGGUGGAAGU GUGGCGACACAUGGAGCUGACAGAUACUAAUCGAUCGAGGACUUAACCAU D26185.1 5S rRNA Bacillus subtilis subsp. subtilis str. 168 0.037420242 1 UUUGGUGGCGAUAGCGAAGAGGUCACACCCGUUCCCAUACCGAACACGGAAGUUAAGCUCUUCAGCGCCGAUGGUAGUCG GGGGUUUCCCCCUGUGAGAGUAGGACGCCGCCAAGC CP019662.1 50S ribosomal protein L2 Bacillus subtilis subsp. subtilis str. 168 0.030335124999999998 1 LEVO MAIKKYKPTSNGRRGMTTSDFAEITTDKPEKSLLAPLHKKGGRNNQGKLTVRHQGGGHKRQYRVIDFKRDKDGIPGRVAT VEYDPNRSANIALINYADGEKRYILAPKGIQVGTEIMSGPEADIKVGNALPLINIPVGTVVHNIELKPGKGGQLVRSAGT SAQVLGKEGKYVLVRLNSGEVRMILSACRASIGQVGNEQHELINIGKAGRSRWKGIRPTVRGSVMNPNDHPHGGGEGRAP IGRKSPMSPWGKPTLGFKTRKKKNKSDKFIVRRRKNK P42919 50S ribosomal protein L3 Bacillus subtilis subsp. subtilis str. 168 0.022723348 1 LEVO MTKGILGRKIGMTQVFAENGDLIPVTVIEAAPNVVLQKKTAENDGYEAIQLGFDDKREKLSNKPEKGHVAKAETAPKRFV KELRGVEMDAYEVGQEVKVEIFSAGEIVDVTGVSKGKGFQGAIKRHGQSRGPMSHGSRYHRRPGSMGPVDPNRVFKGKLL PGRMGGEQITVQNLEIVKVDAERNLLLIKGNVPGAKKSLITVKSAVKSK P42920 50S ribosomal protein L4 Bacillus subtilis subsp. subtilis str. 168 0.022424951 1 LEVO MPKVALYNQNGSTAGDIELNASVFGIEPNESVVFDAILMQRASLRQGTHKVKNRSEVRGGGRKPWRQKGTGRARQGSIRS PQWRGGGVVFGPTPRSYSYKLPKKVRRLAIKSVLSSKVIDNNIIVLEDLTLDTAKTKEMAAILKGLSVEKKALIVTADAN EAVALSARNIPGVTVVEANGINVLDVVNHEKLLITKAAVEKVEEVLA P42921 50S ribosomal protein L5 Bacillus subtilis subsp. subtilis str. 168 0.020177564 1 LEVO MNRLKEKYNKEIAPALMTKFNYDSVMQVPKIEKIVINMGVGDAVQNAKAIDSAVEELTFIAGQKPVVTRAKKSIAGFRLR EGMPIGAKVTLRGERMYDFLDKLISVSLPRVRDFRGVSKKSFDGRGNYTLGIKEQLIFPEIDYDKVTKVRGMDIVIVTTA NTDEEARELLTQVGMPFQK P12877 50S ribosomal protein L6 Bacillus subtilis subsp. subtilis str. 168 0.019543388999999998 1 LEVO MSRVGKKLLEIPSDVTVTLNDNNTVAVKGPKGELTRTFHPDMEIKVEDNVLTVARPSDQKEHRALHGTTRSLLGNMVEGV SKGFERGLELVGVGYRASKSGNKLVLNVGYSHPVEIVPEEGIEIEVPSQTKVVVKGTDKERVGAIAANIRAVRSPEPYKG KGIRYEGEVVRRKEGKSAK P46898 50S ribosomal protein L13 Bacillus subtilis subsp. subtilis str. 168 0.016407104 1 LEVO MRTTPMANASTIERKWLVVDAAGKTLGRLSSEVAAILRGKHKPTYTPHVDTGDHVIIINAEKIELTGKKLTDKIYYRHTQ HPGGLKSRTALEMRTNYPEKMLELAIKGMLPKGSLGRQMFKKLNVYRGSEHPHEAQKPEVYELRG P70974 50S ribosomal protein L14 Bacillus subtilis subsp. subtilis str. 168 0.013175288 1 LEVO MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVKQATPGGVVKKGEVVKAVIVRTKSGARRSDGSYISFD ENACVIIRDDKSPRGTRIFGPVARELRENNFMKIVSLAPEVI P12875 50S ribosomal protein L15 Bacillus subtilis subsp. subtilis str. 168 0.015410693999999999 1 LEVO MKLHELKPSEGSRKTRNRVGRGIGSGNGKTAGKGHKGQNARSGGGVRPGFEGGQMPLFQRLPKRGFTNINRKEYAVVNLD KLNGFAEGTEVTPELLLETGVISKLNAGVKILGNGKLEKKLTVKANKFSASAKEAVEAAGGTAEVI P19946 50S ribosomal protein L16 Bacillus subtilis subsp. subtilis str. 168 0.016223049 1 LEVO MLLPKRVKYRREHRGKMRGRAKGGTEVHFGEFGIQALEASWITNRQIEAARIAMTRYMKRGGKVWIKIFPSKPYTAKPLE VRMGSGKGAPEGWVAVVKPGKVLFEISGVSEEVAREALRLASHKLPIKTKFVKREEIGGESNES P14577 50S ribosomal protein L17 Bacillus subtilis subsp. subtilis str. 168 0.013774806 1 LEVO MSYRKLGRTSAQRKAMLRDLTTDLIINERIETTETRAKELRSVVEKMITLGKRGDLHARRQAAAYIRNEVANEENNQDAL QKLFSDIATRYEERQGGYTRIMKLGPRRGDGAPMAIIELV P20277 50S ribosomal protein L18 Bacillus subtilis subsp. subtilis str. 168 0.012993828999999998 1 LEVO MITKTSKNAARLKRHARVRAKLSGTAERPRLNVFRSNKHIYAQIIDDVNGVTLASASTLDKDLNVESTGDTSAATKVGEL VAKRAAEKGISDVVFDRGGYLYHGRVKALADAAREAGLKF P46899 50S ribosomal protein L19 Bacillus subtilis subsp. subtilis str. 168 0.013416852999999998 1 LEVO MQKLIEDITKEQLRTDLPAFRPGDTLRVHVKVVEGNRERIQIFEGVVIKRRGGGISETFTVRKISYGVGVERTFPVHTPK IAKIEVVRYGKVRRAKLYYLRELRGKAARIKEIRR O31742 50S ribosomal protein L20 Bacillus subtilis subsp. subtilis str. 168 0.013669189 1 LEVO MPRVKGGTVTRKRRKKVLKLAKGYFGSKHTLYKVANQQVMKSGNYAFRDRRQKKRDFRKLWITRINAAARMNGLSYSRLM HGLKLSGIEVNRKMLADLAVNDLTAFNQLADAAKAQLNK P55873 50S ribosomal protein L21 Bacillus subtilis subsp. subtilis str. 168 0.011296081 1 LEVO MYAIIKTGGKQIKVEEGQTVYIEKLAAEAGETVTFEDVLFVGGDNVKVGNPTVEGATVTAKVEKQGRAKKITVFRYKPKK NVHKKQGHRQPYTKVTIEKINA P26908 50S ribosomal protein L22 Bacillus subtilis subsp. subtilis str. 168 0.012481608 1 LEVO MQAKAVARTVRIAPRKARLVMDLIRGKQVGEAVSILNLTPRAASPIIEKVLKSAIANAEHNYEMDANNLVISQAFVDEGP TLKRFRPRAMGRASQINKRTSHITIVVSEKKEG P42060 50S ribosomal protein L23 Bacillus subtilis subsp. subtilis str. 168 0.010978813 1 LEVO MKDPRDVLKRPVITERSADLMTEKKYTFEVDVRANKTEVKDAVESIFGVKVDKVNIMNYKGKSKRVGRYTGMTSRRRKAI VKLTADSKEIEIFEA P42924 50S ribosomal protein L24 Bacillus subtilis subsp. subtilis str. 168 0.01116612 1 LEVO MHVKKGDKVMVISGKDKGKQGTILAAFPKKDRVLVEGVNMVKKHSKPTQANPQGGISNQEAPIHVSNVMPLDPKTGEVTR VGYKVEDGKKVRVAKKSGQVLDK P0CI78 50S ribosomal protein L27 Bacillus subtilis subsp. subtilis str. 168 0.010391854999999998 1 LEVO MLRLDLQFFASKKGVGSTKNGRDSEAKRLGAKRADGQFVTGGSILYRQRGTKIYPGENVGRGGDDTLFAKIDGTVKFERF GRDRKKVSVYPVAQ P05657 50S ribosomal protein L32 Bacillus subtilis subsp. subtilis str. 168 0.006745073 1 LEVO MAVPFRRTSKMKKRLRRTHFKLNVPGMTECPSCGEMKLSHRVCKACGSYNGKDINVKSN O34687 50S ribosomal protein L33 1 Bacillus subtilis subsp. subtilis str. 168 0.005915875 1 LEVO MRVNITLACTECGERNYISKKNKRNNPDRVEFKKYCPRDKKSTLHRETK P56849 50S ribosomal protein L34 Bacillus subtilis subsp. subtilis str. 168 0.005271332 1 LEVO MKRTFQPNNRKRSKVHGFRSRMSSKNGRLVLARRRRKGRKVLSA P05647 50S ribosomal protein L35 Bacillus subtilis subsp. subtilis str. 168 0.0075811279999999995 1 LEVO MPKMKTHRGSAKRFKKTGSGKLKRSHAYTSHLFANKSQKQKRKLRKSAVVSAGDFKRIKQMLANIK P55874 50S ribosomal protein L36 Bacillus subtilis subsp. subtilis str. 168 0.004318422 1 LEVO MKVRPSVKPICEKCKVIRRKGKVMVICENPKHKQKQG P20278 50S ribosomal protein L28 Bacillus subtilis subsp. subtilis str. 168 0.006826144 1 LEVO MARKCVITGKKTTAGNNRSHAMNASKRTWGANLQKVRILVNGKPKKVYVSARALKSGKVERV P37807 50S ribosomal protein L29 Bacillus subtilis subsp. subtilis str. 168 0.007728029 1 LEVO MKANEIRDLTTAEIEQKVKSLKEELFNLRFQLATGQLENTARIREVRKAIARMKTVIREREIAANK P12873 50S ribosomal protein L30 Bacillus subtilis subsp. subtilis str. 168 0.006650795 1 LEVO MAKLEITLKRSVIGRPEDQRVTVRTLGLKKTNQTVVHEDNAAIRGMINKVSHLVSVKEQ P19947 MAGNESIUM ION 2.4305e-05 221 MG ZINC ION 6.5409e-05 3 ZN singleParticle particle 7.5 FEI TALOS ARCTICA FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 FEI FALCON III (4k x 4k) 23.94 1 C1 3.07 FSC 0.143 CUT-OFF 57683 OTHER MAXIMUM LIKELIHOOD 3J3V PDB experimental model