ER microsome released with the code in (Martinez-Sanchez et al., Nature Methods)

2020-02-12 REL PDBe PDBe PDBe 2019-11-04 2019-12-11 2019-12-11 2020-02-12 Seneca Foundation Spain ER microsome released with the code in (Martinez-Sanchez et al., Nature Methods) Martinez-Sanchez A Lucic V Pfeffer S Forster F Martinez-Sanchez A Kochovski Z Laugks U Meyer Zum Alten Borgloh J Chakraborty S Pfeffer S Baumeister W Lucic V Template-free detection and classification of membrane-bound complexes in cryo-electron tomograms. Nat.Methods US 17 209 216 2020 31907446 doi:10.1038/s41592-019-0675-5 1548-7105 Pfeffer S Forster F Structure of the native Sec61 protein-conducting channel Nature Communications UK 6 2015 2041-1723 EMD-3072 other EM volume

Same sample (original publication)

EMD-0074 other EM volume

Averged density obtained from this sample

EMD-0084 other EM volume

Averged density obtained from this sample

EMD-0075 other EM volume

Averged density obtained from this sample

EMD-0085 other EM volume

Averged density obtained from this sample

EMD-0086 other EM volume

Averged density obtained from this sample

EMD-10449 other EM volume

Same sample and same publication

EMD-10450 other EM volume

Same sample and same publication

EMD-10451 associated EM volume EMD-0087 other EM volume

Averaged density obtained from this sample

Representative tomogram of a canine pancreatic rough Endoplasmic Reticulum vesicle. Representative tomogram of a canine pancreatic rough Endoplasmic Reticulum vesicle. 0 Canis lupus familiaris pancreas tomography particle 2.00 7.6 20.0 Hepes 50.0 KCl 2.0 MgCl2 ETHANE-PROPANE 70 FEI VITROBOT MARK IV

Blot 3 seconds before plunging..

Aurion, Wageningen, The Netherlands 10 NO SECTIONING FEI TITAN KRIOS OTHER OTHER FIELD EMISSION GUN 300 70.0 2.7 3.0 4.0 42000.0 NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 1.5 1

Reconstruction of a single microsome after two binning steps (from 2.62A/pixel to 10.48A/pixel)

PyTom 61 PyTom emd_10451.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 166 166 50 0 0 0 166 166 50 1739.6799 1739.6799 524.0 90.0 90.0 90.0 X Y Z -1.323595 0.82137954 0.0010220177 0.097294405 10.48 10.48 10.48 AUTHOR ::::EMDATABANK.org::::EMD-10451:::: ER microsome used in the code released in (Martinez-Sanchez et al., Nature Methods)