2020-02-12 REL PDBe PDBe PDBe 2019-10-31 2019-12-11 2019-12-11 2020-02-12 Seneca Foundation Spain Putative in situ ER membrane bound major histocompatibility peptide loading complex (PLC) Martinez-Sanchez A Lucic V Chakraborty S Martinez-Sanchez A Kochovski Z Laugks U Meyer Zum Alten Borgloh J Chakraborty S Pfeffer S Baumeister W Lucic V Template-free detection and classification of membrane-bound complexes in cryo-electron tomograms. Nat.Methods US 17 209 216 2020 31907446 doi:10.1038/s41592-019-0675-5 1548-7105 EMD-10435 other EM volume
Same sample and putatively a similar complex
 EMD-10436 associated EM volume Intact P19 cells Intact P19 cells 0 Mus musculus teratocarcinoma subtomogramAveraging cell 7.4 ETHANE-PROPANE 90 310.15 FEI VITROBOT MARK IV
FEI TITAN KRIOS OTHER OTHER FIELD EMISSION GUN 300 70.0 2.7 0.2 0.25 42000.0 NITROGEN VOLTA PHASE PLATE 20 GATAN K2 SUMMIT (4k x 4k) COUNTING 1.0 1 C1 32.18 FSC 0.143 CUT-OFF RELION 2.1 177 12 172000 Template-free IMOD PySeg for particle picking and IMOD for subvolume reconstruction
PySeg software, automated and comprehensive detection of heterogeneous membrane-bound complexes.
 RELION 2.1 Before final classification with RELION, PySeg unsuprevised classification was used. MAXIMUM LIKELIHOOD RELION 2.1 emd_10436.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 160 160 160 0 0 0 160 160 160 547.2 547.2 547.2 90.0 90.0 90.0 X Y Z -0.5688855 0.7600839 0.00051101693 0.058727626 3.42 3.42 3.42 0.067 AUTHOR Putative in situ ER membrane bound major histocompatibility peptide loading complex (PLC)