2021-04-14 REL PDBe PDBe PDBe 2019-10-09 2020-10-14 2020-10-14 2021-04-14 French National Research Agency ANR-17CE11-0028 France CryoEM structure of MexB in nanodisc Glavier M Puvanendran D Salvador D Decossas M Phan G Garnier C Frezza E Cece Q Schoehn G Picard M Taveau J-C Daury L Broutin I Lambert O Glavier M Puvanendran D Salvador D Decossas M Phan G Garnier C Frezza E Cece Q Schoehn G Picard M Taveau JC Daury L Broutin I Lambert O Antibiotic export by MexB multidrug efflux transporter is allosterically controlled by a MexA-OprM chaperone-like complex. Nat Commun UK 11 4948 4948 2020 33009415 doi:10.1038/s41467-020-18770-5 2041-1723 EMD-10371 associated EM volume 6t7s FULLOVERLAP MexB MexB 0 1 Pseudomonas aeruginosa Escherichia coli MEXB Pseudomonas aeruginosa Escherichia coli LEVO MSKFFIDRPIFAWVIALVIMLAGGLSILSLPVNQYPAIAPPAIAVQVSYPGASAETVQDT VVQVIEQQMNGIDNLRYISSESNSDGSMTITVTFEQGTDPDIAQVQVQNKLQLATPLLPQ EVQRQGIRVTKAVKNFLMVVGVVSTDGSMTKEDLSNYIVSNIQDPLSRTKGVGDFQVFGS QYSMRIWLDPAKLNSYQLTPGDVSSAIQAQNVQISSGQLGGLPAVKGQQLNATIIGKTRL QTAEQFENILLKVNPDGSQVRLKDVADVGLGGQDYSINAQFNGSPASGIAIKLATGANAL DTAKAIRQTIANLEPFMPQGMKVVYPYDTTPVVSASIHEVVKTLGEAILLVFLVMYLFLQ NFRATLIPTIAVPVVLLGTFGVLAAFGFSINTLTMFGMVLAIGLLVDDAIVVVENVERVM AEEGLSPREAARKSMGQIQGALVGIAMVLSAVFLPMAFFGGSTGVIYRQFSITIVSAMAL SVIVALILTPALCATMLKPIEKGDHGEHKGGFFGWFNRMFLSTTHGYERGVASILKHRAP YLLIYVVIVAGMIWMFTRIPTAFLPDEDQGVLFAQVQTPPGSSAERTQVVVDSMREYLLE KESSSVSSVFTVTGFNFAGRGQSSGMAFIMLKPWEERPGGENSVFELAKRAQMHFFSFKD AMVFAFAPPSVLELGNATGFDLFLQDQAGVGHEVLLQARNKFLMLAAQNPALQRVRPNGM SDEPQYKLEIDDEKASALGVSLADINSTVSIAWGSSYVNDFIDRGRVKRVYLQGRPDARM NPDDLSKWYVRNDKGEMVPFNAFATGKWEYGSPKLERYNGVPAMEILGEPAPGLSSGDAM AAVEEIVKQLPKGVGYSWTGLSYEERLSGSQAPALYALSLLVVFLCLAALYESWSIPFSV MLVVPLGVIGALLATSMRGLSNDVFFQVGLLTTIGLSAKNAILIVEFAKELHEQGKGIVE AAIEACRMRLRPIVMTSLAFILGVVPLAISTGAGSGSQHAIGTGVIGGMVTATVLAIFWV PLFYVAVSTLFKDEASKQQASVEKGQ singleParticle particle 7.4 ETHANE FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 QUANTUM (4k x 4k) 42.0 1 212667 Gctf C1 4.5 FSC 0.143 CUT-OFF RELION 41604 MAXIMUM LIKELIHOOD RELION MAXIMUM LIKELIHOOD RELION emd_10371.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 408.0 408.0 408.0 90.0 90.0 90.0 X Y Z -0.029316468 0.067547865 -3.3355784e-06 0.0027080537 1.36 1.36 1.36 0.02 AUTHOR CryoEM structure of RND transport MexB trimer from Pseudomonas aeruginosa stabilized in nanodisc