REL PDBe PDBe PDBe 2003-02-28 2003-02-28 2003-03-28 2016-04-13 Nucleotide-induced conformations in the neck region of dimeric kinesin. Skiniotis G Skiniotis G Surrey T Altmann S Gross H Song YH Mandelkow E Hoenger A Nucleotide-induced conformations in the neck region of dimeric kinesin. EMBO J. 22 1518 1528 2003 12660159 doi:10.1093/emboj/cdg164 rat kinesin construct rK379 rat kinesin construct rK379 dimer 2 rat kinesin Rattus norvegicus rat kinesin 0.085 1 dimer true Escherichia coli pET3 tubulin Rattus norvegicus rat kinesin brain neuronal cells cytoplasm 0.11 1 hetero-dimer false helical filament 0.5 6.8
PIPES 80 mM, MgCl2 1 mM, GTP 1 mM, Taxol 20 uM, DMSO 7.5%
 NEGATIVE
ice-embeded
holey grids
 ETHANE 93 HOMEMADE PLUNGER
Vitrification instrument: self made
 FEI/PHILIPS CM200FEG/ST FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2 2.0 2.5 38000.0 GATAN LIQUID NITROGEN 95 was corrected at 180,000 times mag. KODAK SO-163 FILM ZEISS SCAI 21 17 5 8. side-entry 0 0 C1 OTHER 25.0 FSC 0.5 CUT-OFF PHOELIX, SUPREME
Final map from 33 averaged datasets = 17 helical tubes
 emd_1037.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 100 100 100 0 0 0 100 100 100 552.6 552.6 552.6 90 90 90 X Y Z 0.0 100.0 44.555100000000003 9.680400000000001 5.526 5.526 5.526 65.400000000000006 Rat Kinesin Dimer rK379,Kozielski et al. 1997, complexed to microtubules in the presence of AMP-PNP
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