2019-12-04 REL PDBe PDBe PDBe 2019-09-09 2019-11-13 2019-11-13 2019-12-04 Medical Research Council (United Kingdom) MC_UP_1201/8 United Kingdom Wellcome Trust 202231/Z/16/Z United Kingdom Medical Research Council (United Kingdom) MC_UP_1201/10 United Kingdom CLEM of resin-embedded scs2/22 tcb1/2/3 deletion mutant yeast cell expressing GFP-Ist2 shown in Figure 2F of publication Hoffmann PC Bharat TAM Wozny MR Boulanger J Miller EA Kukulski W Hoffmann PC Bharat TAM Wozny MR Boulanger J Miller EA Kukulski W Tricalbins Contribute to Cellular Lipid Flux and Form Curved ER-PM Contacts that Are Bridged by Rod-Shaped Structures. Dev.Cell US 51 488 502.e8 2019 31743663 doi:10.1016/j.devcel.2019.09.019 1534-5807 EMD-10299 other EM volume EMD-10287 other EM volume EMD-10300 other EM volume EMD-10301 associated EM volume Correlative FM and ET of resin-embedded scs2/22 tcb1/2/3 deletion mutant yeast cell expressing GFP-Ist2 Correlative FM and ET of resin-embedded scs2/22 tcb1/2/3 deletion mutant yeast cell expressing GFP-Ist2 0 Saccharomyces cerevisiae tomography cell 7.4 NONE Uranyl Acetate
0.03-0.05 % uranyl acetate during freeze substitution and post-staining of sections with Reynold's lead citrate
 Lowicryl HM20 NITROGEN
Electron Microscopy Sciences 15 Life Technologies 50 OTHER
The value given for _emd_high_pressure_freezing.instrument is Leica EM HPM100. This is not in a list of allowed values set(['LEICA EM PACT2', 'LEICA EM PACT', 'EMS-002 RAPID IMMERSION FREEZER', 'OTHER', 'LEICA EM HPM100', 'BAL-TEC HPM 010']) so OTHER is written into the XML file.
 Reichert Ultracut E 298 270 FEI TECNAI F20 OTHER BRIGHT FIELD FIELD EMISSION GUN 200
STEM mode on an axial brightfield detector with a high-tilt tomography holder (Fischione Instruments, Model 2020)
OTHER 1000.0
ET was done in STEM mode on an axial brightfield detector with a high-tilt tomography holder at 1.102 nm pixel size with a camera length of 200 mm. [NOTE: Electron dose unknown. Value specified here is a dummy]
 1 eTomo
239 tilted images used from two axes
239 eTomo emd_10301.map.gz 1 IMAGE STORED AS SIGNED BYTE 2048 2048 204 0 0 -102 2048 2048 204 22568.96 22568.96 2248.08 90.0 90.0 90.0 X Y Z -128 127 46.536835 3.915299 11.02 11.02 11.02 AUTHOR CLEM of resin-embedded GFP-Ist2 scs2/22 tcb1/2/3 deletion mutant yeast cell shown in Figure 2F of publication