2024-05-22 REL PDBe PDBe PDBe 2019-07-01 2019-07-24 2019-08-28 2024-05-22 European Union 665385 Austria Structure of ovine transhydrogenase in the apo state Kampjut D Sazanov LA mitochondrial, proton-translocating, nicotinamide nucleotide transhydrogenase, MEMBRANE PROTEIN Kampjut D Sazanov LA Structure and mechanism of mitochondrial proton-translocating transhydrogenase. Nature UK 573 291 295 2019 31462775 doi:10.1038/s41586-019-1519-2 1476-4687 0006 NATUAS 6s59 FULLOVERLAP Nicotinamide nucleotide transhydrogenase in the apo state Nicotinamide nucleotide transhydrogenase in the apo state 0 1 Ovis aries 0.22 Nicotinamide nucleotide transhydrogenase Ovis aries heart 0.11401030499999999 2 LEVO MANLLKTVVTGCSCPFLSNLGSCKVLPGKKNFLRTFHTHRILWCKAPVKPGIPYKQLTVGVPKEIFQNEKRVALSPAGVQ ALVKQGFNVVVESGAGEASKFSDDHYRAAGAQIQGAKEVLASDLVVKVRAPMLNPTLGIHEADLLKTSGTLISFIYPAQN PDLLNKLSKRNTTVLAMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANHFGRFFTGQITAAGKVPPAKILIVGGGVAGL ASAGAAKSMGAIVRGFDTRAAALEQFKSLGAEPLEVDLKESGEGQGGYAKEMSKEFIEAEMKLFAQQCKEVDILISTALI PGKKAPILFNKEMIESMKEGSVVVDLAAEAGGNFETTKPGELYVHKGITHIGYTDLPSRMATQASTLYSNNITKLLKAIS PDKDNFYFEVKDDFDFGTMGHVIRGTVVMKDGQVIFPAPTPKNIPQGAPVKQKTVAELEAEKAATITPFRKTMTSASVYT AGLTGILGLGIAAPNLAFSQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLVLMGGHLYPSTTSQGLA ALATFISSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPAGTFVGGYLASLYSGYNIEQIMYLGSGLCCVGALAGLST QGTARLGNALGMIGVAGGLAATLGGLKPCPELLAQMSGAMALGGTIGLTIAKRIQISDLPQLVAAFHSLVGLAAVLTCIA EYIIEYPHFATDAAANLTKIVAYLGTYIGGVTFSGSLVAYGKLQGILKSAPLLLPGRHLLNAGLLAASVGGIIPFMMDPS FTTGITCLGSVSALSAVMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNNLLTIVGALIGSSGAILSYIMCVAM NRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLSEQGKKVRF GIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIV MKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK W5PFI3 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE 0.0007901449999999999 8 PC1 singleParticle particle 5 7.4 20.0 HEPES 50.0 NaCl Sodium chloride 1.0 EDTA Quantifoil R0.6/1 COPPER 300 ETHANE 100 277 FEI VITROBOT MARK IV
4-6 s blotting time.
Sample was purified in LMNG (final conc. 0.06%) and contained 0.05% CHAPS as a secondary detergent.
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K3 (6k x 4k) SUPER-RESOLUTION 1 760 3.25 90.0 1 500001
Reconstruction from a previous dataset
 1 C1 3.7 FSC 0.143 CUT-OFF RELION 3.0 67908 MAXIMUM LIKELIHOOD RELION 3.0 MAXIMUM LIKELIHOOD RELION 3.0 emd_10099.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 147 149 167 0 0 0 167 149 147 140.28 125.159996 123.479996 90.0 90.0 90.0 Z Y X -0.04270431 0.08285316 0.0020778421 0.0064960094 0.84 0.84 0.84 0.023 AUTHOR Density for apo-NNT. dI density from consensus full NNT refinement and dIIdIII density from focused refinement. 6QTI PDB experimental model AB INITIO MODEL Correlation coefficient, EMRinger score REAL 137.0