2024-05-29 REL PDBj PDBj PDBj 2019-08-05 2020-08-12 2020-08-12 2024-05-29 Japan Agency for Medical Research and Development (AMED) JP19am0101071 Japan Adachi N Yamaguchi T Moriya T Kawasaki M Koiwai K Shinoda A Yamada Y Yumoto F Kohzuma T Senda T Cu containing nitrite reductase, OXIDOREDUCTASE Adachi N Yamaguchi T Moriya T Kawasaki M Koiwai K Shinoda A Yamada Y Yumoto F Kohzuma T Senda T J.Struct.Biol. US 213 107768 107768 2021 34217801 doi:10.1016/j.jsb.2021.107768 1095-8657 0803 JSBIEM 6kng FULLOVERLAP Nitrite reductase Nitrite reductase 0 1 Achromobacter cycloclastes 0.11 Copper-containing nitrite reductase Achromobacter cycloclastes 0.036621332 3 LEVO VDISTLPRVKVDLVKPPFVHAHDQVAKTGPRVVEFTMTIEEKKLVIDREGTEIHAMTFNGSVPGPLMVVHENDYVELRLI NPDTNTLLHNIDFHAATGALGGGALTQVNPGEETTLRFKATKPGVFVYHCAPEGMVPWHVTSGMNGAIMVLPRDGLKDEK GQPLTYDKIYYVGEQDFYVPKDEAGNYKKYETPGEAYEDAVKAMRTLTPTHIVFNGAVGALTGDHALTAAVGERVLVVHS QANRDTRPHLIGGHGDYVWATGKFRNPPDLDQETWLIPGGTAGAAFYTFRQPGVYAYVNHNLIEAFELGAAGHFKVTGEW NDDLMTSVVKPASM P25006 1.7.2.1 COPPER (II) ION 6.354599999999999e-05 6 CU singleParticle particle 1.1 8.1 100.0 pottasium phosphate Quantifoil R1.2/1.3 COPPER 300 CARBON HOLEY GLOW DISCHARGE 30 AIR

The grid was washed by acetone prior to use.

ETHANE 100 291 FEI VITROBOT MARK IV

Blotting time was 15 seconds..

This sample was mono-disperse.

FEI TALOS ARCTICA FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 50.0 2.7 1.0 3.0 120000.0 NITROGEN FEI FALCON III (4k x 4k) COUNTING 1 694 56.37 50.0 1

An ab initio model was generated using RELION3's own implementation of Stochastic Gradient Descent (SGD) algorithm and low-pass filtered to 25 A for use as an initial model for 3D refinement.

C3 2.85 FSC 0.143 CUT-OFF RELION 3 89513 MAXIMUM LIKELIHOOD RELION 3 MAXIMUM LIKELIHOOD RELION 3