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            <date>2024-03-27</date>
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            <deposition>2019-07-20</deposition>
            <header_release>2019-10-02</header_release>
            <map_release>2019-10-02</map_release>
            <update>2024-03-27</update>
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                <funding_body>Ministry of Science and Technology (China)</funding_body>
                <code>2016YFA0501902</code>
                <country>China</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Ministry of Science and Technology (China)</funding_body>
                <code>2016YFA0501102</code>
                <country>China</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Natural Science Foundation of China</funding_body>
                <code>31570730</code>
                <country>China</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Natural Science Foundation of China</funding_body>
                <code>91853113</code>
                <country>China</country>
            </grant_reference>
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        <title>200kV MicroED structure of FUS (37-42) SYSGYS solved from single crystal at 0.67 A</title>
        <authors_list>
            <author>Zhou H</author>
            <author>Luo F</author>
        </authors_list>
        <keywords>FUS, MicroED, Ultrahigh resolution, RNA BINDING PROTEIN</keywords>
    </admin>
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        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Zhou H</author>
                    <author order="2">Luo F</author>
                    <author order="3">Luo Z</author>
                    <author order="4">Li D</author>
                    <author order="5">Liu C</author>
                    <author ORCID="0000-0002-8451-9947" order="6">Li X</author>
                    <title>Programming Conventional Electron Microscopes for Solving Ultrahigh-Resolution Structures of Small and Macro-Molecules.</title>
                    <journal_abbreviation>Anal.Chem.</journal_abbreviation>
                    <country>US</country>
                    <volume>91</volume>
                    <first_page>10996</first_page>
                    <last_page>11003</last_page>
                    <year>2019</year>
                    <external_references type="PUBMED">31334636</external_references>
                    <external_references type="DOI">doi:10.1021/acs.analchem.9b01162</external_references>
                    <external_references type="ISSN">1520-6882</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
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                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
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                <natural_source database="NCBI">
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                <molecular_weight>
                    <theoretical units="MDa">0.000662648</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>SYSGYS</string>
                    <external_references type="UNIPROTKB">P35637</external_references>
                </sequence>
            </protein_or_peptide>
            <ligand macromolecule_id="2">
                <name>water</name>
                <molecular_weight>
                    <theoretical units="MDa">1.8015e-05</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <formula>HOH</formula>
            </ligand>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>electronCrystallography</method>
            <aggregation_state>threeDArray</aggregation_state>
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                        <cryogen_name>ETHANE</cryogen_name>
                    </vitrification>
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                <crystallography_microscopy microscopy_id="1">
                    <microscope>FEI TECNAI F20</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>DIFFRACTION</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">200</acceleration_voltage>
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                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN ULTRASCAN 4000 (4k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">0.05</average_electron_dose_per_image>
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                    <camera_length units="mm">520</camera_length>
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                    <resolution units="Å" res_type="BY AUTHOR">0.67</resolution>
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                    <r_sym>0.123</r_sym>
                    <r_merge>0.123</r_merge>
                    <overall_phase_error>40.93</overall_phase_error>
                    <overall_phase_residual>40.93</overall_phase_residual>
                    <phase_error_rejection_criteria>1</phase_error_rejection_criteria>
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                        <shell shell_id="1">
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                            <low_resolution units="Å">0.69</low_resolution>
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