2024-10-23
REL
PDBj
PDBj
PDBj
2019-01-15
2019-04-10
2019-04-24
2024-10-23
National Natural Science Foundation of China
31430020
China
National Natural Science Foundation of China
31621092
China
Ministry of Science and Technology (China)
2016YFA0501100
China
Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom
Wan R
Bai R
spliceosme, B* complex, branching, snRNP, U snRNA, SPLICING
Wan R
Bai R
Yan C
Lei J
Shi Y
Structures of the Catalytically Activated Yeast Spliceosome Reveal the Mechanism of Branching.
Cell
177
339
351.e13
2019
30879786
doi:10.1016/j.cell.2019.02.006
1097-4172
6j6g
FULLOVERLAP
catalytically activated spliceosome B*-a2 complex
catalytically activated spliceosome B*-a2 complex
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Saccharomyces cerevisiae S288c
S288c
Pre-mRNA-splicing factor 8
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.279867469
1
LEVO
MSGLPPPPPGFEEDSDLALPPPPPPPPGYEIEELDNPMVPSSVNEDTFLPPPPPPPSNFEINAEEIVDFTLPPPPPPPGL
DELETKAEKKVELHGKRKLDIGKDTFVTRKSRKRAKKMTKKAKRSNLYTPKAEMPPEHLRKIINTHSDMASKMYNTDKKA
FLGALKYLPHAILKLLENMPHPWEQAKEVKVLYHTSGAITFVNETPRVIEPVYTAQWSATWIAMRREKRDRTHFKRMRFP
PFDDDEPPLSYEQHIENIEPLDPINLPLDSQDDEYVKDWLYDSRPLEEDSKKVNGTSYKKWSFDLPEMSNLYRLSTPLRD
EVTDKNYYYLFDKKSFFNGKALNNAIPGGPKFEPLYPREEEEDYNEFNSIDRVIFRVPIRSEYKVAFPHLYNSRPRSVRI
PWYNNPVSCIIQNDEEYDTPALFFDPSLNPIPHFIDNNSSLNVSNTKENGDFTLPEDFAPLLAEEEELILPNTKDAMSLY
HSPFPFNRTKGKMVRAQDVALAKKWFLQHPDEEYPVKVKVSYQKLLKNYVLNELHPTLPTNHNKTKLLKSLKNTKYFQQT
TIDWVEAGLQLCRQGHNMLNLLIHRKGLTYLHLDYNFNLKPTKTLTTKERKKSRLGNSFHLMRELLKMMKLIVDTHVQFR
LGNVDAFQLADGIHYILNHIGQLTGIYRYKYKVMHQIRACKDLKHIIYYKFNKNLGKGPGCGFWQPAWRVWLNFLRGTIP
LLERYIGNLITRQFEGRSNEIVKTTTKQRLDAYYDLELRNSVMDDILEMMPESIRQKKARTILQHLSEAWRCWKANIPWD
VPGMPAPIKKIIERYIKSKADAWVSAAHYNRERIKRGAHVEKTMVKKNLGRLTRLWIKNEQERQRQIQKNGPEITPEEAT
TIFSVMVEWLESRSFSPIPFPPLTYKNDTKILVLALEDLKDVYASKVRLNASEREELALIEEAYDNPHDTLNRIKKYLLT
QRVFKPVDITMMENYQNISPVYSVDPLEKITDAYLDQYLWYEADQRKLFPNWIKPSDSEIPPLLVYKWTQGINNLSEIWD
VSRGQSAVLLETTLGEMAEKIDFTLLNRLLRLIVDPNIADYITAKNNVVINFKDMSHVNKYGLIRGLKFASFIFQYYGLV
IDLLLLGQERATDLAGPANNPNEFMQFKSKEVEKAHPIRLYTRYLDRIYMLFHFEEDEGEELTDEYLAENPDPNFENSIG
YNNRKCWPKDSRMRLIRQDVNLGRAVFWEIQSRVPTSLTSIKWENAFVSVYSKNNPNLLFSMCGFEVRILPRQRMEEVVS
NDEGVWDLVDERTKQRTAKAYLKVSEEEIKKFDSRIRGILMASGSTTFTKVAAKWNTSLISLFTYFREAIVATEPLLDIL
VKGETRIQNRVKLGLNSKMPTRFPPAVFYTPKELGGLGMISASHILIPASDLSWSKQTDTGITHFRAGMTHEDEKLIPTI
FRYITTWENEFLDSQRVWAEYATKRQEAIQQNRRLAFEELEGSWDRGIPRISTLFQRDRHTLAYDRGHRIRREFKQYSLE
RNSPFWWTNSHHDGKLWNLNAYRTDVIQALGGIETILEHTLFKGTGFNSWEGLFWEKASGFEDSMQFKKLTHAQRTGLSQ
IPNRRFTLWWSPTINRANVYVGFLVQLDLTGIFLHGKIPTLKISLIQIFRAHLWQKIHESIVFDICQILDGELDVLQIES
VTKETVHPRKSYKMNSSAADITMESVHEWEVSKPSLLHETNDSFKGLITNKMWFDVQLRYGDYDSHDISRYVRAKFLDYT
TDNVSMYPSPTGVMIGIDLAYNMYDAYGNWFNGLKPLIQNSMRTIMKANPALYVLRERIRKGLQIYQSSVQEPFLNSSNY
AELFNNDIKLFVDDTNVYRVTVHKTFEGNVATKAINGCIFTLNPKTGHLFLKIIHTSVWAGQKRLSQLAKWKTAEEVSAL
VRSLPKEEQPKQIIVTRKAMLDPLEVHMLDFPNIAIRPTELRLPFSAAMSIDKLSDVVMKATEPQMVLFNIYDDWLDRIS
SYTAFSRLTLLLRALKTNEESAKMILLSDPTITIKSYHLWPSFTDEQWITIESQMRDLILTEYGRKYNVNISALTQTEIK
DIILGQNIKAPSVKRQKMAELEAARSEKQNDEEAAGASTVMKTKTINAQGEEIVVVASADYESQTFSSKNEWRKSAIANT
LLYLRLKNIYVSADDFVEEQNVYVLPKNLLKKFIEISDVKIQVAAFIYGMSAKDHPKVKEIKTVVLVPQLGHVGSVQISN
IPDIGDLPDTEGLELLGWIHTQTEELKFMAASEVATHSKLFADKKRDCIDISIFSTPGSVSLSAYNLTDEGYQWGEENKD
IMNVLSEGFEPTFSTHAQLLLSDRITGNFIIPSGNVWNYTFMGTAFNQEGDYNFKYGIPLEFYNEMHRPVHFLQFSELAG
DEELEAEQIDVFS
P33334
Pre-mRNA-splicing factor SNU114
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.114174008
1
LEVO
MEGDDLFDEFGNLIGVDPFDSDEEESVLDEQEQYQTNTFEGSGNNNEIESRQLTSLGSKKELGISLEHPYGKEVEVLMET
KNTQSPQTPLVEPVTERTKLQEHTIFTQLKKNIPKTRYNRDYMLSMANIPERIINVGVIGPLHSGKTSLMDLLVIDSHKR
IPDMSKNVELGWKPLRYLDNLKQEIDRGLSIKLNGSTLLCTDLESKSRMINFLDAPGHVNFMDETAVALAASDLVLIVID
VVEGVTFVVEQLIKQSIKNNVAMCFVINKLDRLILDLKLPPMDAYLKLNHIIANINSFTKGNVFSPIDNNIIFASTKLGF
TFTIKEFVSYYYAHSIPSSKIDDFTTRLWGSVYYHKGNFRTKPFENVEKYPTFVEFILIPLYKIFSYALSMEKDKLKNLL
RSNFRVNLSQEALQYDPQPFLKHVLQLIFRQQTGLVDAITRCYQPFELFDNKTAHLSIPGKSTPEGTLWAHVLKTVDYGG
AEWSLVRIYSGLLKRGDTVRILDTSQSESRQKRQLHDISKTETSNEDEDEDDETPSCEVEEIGLLGGRYVYPVHEAHKGQ
IVLIKGISSAYIKSATLYSVKSKEDMKQLKFFKPLDYITEAVFKIVLQPLLPRELPKLLDALNKISKYYPGVIIKVEESG
EHVILGNGELYMDCLLYDLRASYAKIEIKISDPLTVFSESCSNESFASIPVSNSISRLGEENLPGLSISVAAEPMDSKMI
QDLSRNTLGKGQNCLDIDGIMDNPRKLSKILRTEYGWDSLASRNVWSFYNGNVLINDTLPDEISPELLSKYKEQIIQGFY
WAVKEGPLAEEPIYGVQYKLLSISVPSDVNIDVMKSQIIPLMKKACYVGLLTAIPILLEPIYEVDITVHAPLLPIVEELM
KKRRGSRIYKTIKVAGTPLLEVRGQVPVIESAGFETDLRLSTNGLGMCQLYFWHKIWRKVPGDVLDKDAFIPKLKPAPIN
SLSRDFVMKTRRRKGISTGGFMSNDGPTLEKYISAELYAQLRENGLVP
P36048
Pre-mRNA-splicing factor CWC21
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.015793596
1
LEVO
MSYNGIGLKSAKGSSTSGHVQRSLASNNRRRPQGSQQQRQQRQNAIKKASHDKASRPLAVQKQIETHMEKREIEVQVSEL
RDRLEEEETLSEEQIDKKCEALRAKLTNEWQEQQRMSSLYTPRKARLTEEQHRHE
Q03375
Pre-mRNA-splicing factor PRP46
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.050771289
1
LEVO
MDGNDHKVENLGDVDKFYSRIRWNNQFSYMATLPPHLQSEMEGQKSLLMRYDTYRKESSSFSGEGKKVTLQHVPTDFSEA
SQAVISKKDHDTHASAFVNKIFQPEVAEELIVNRYEKLLSQRPEWHAPWKLSRVINGHLGWVRCVAIDPVDNEWFITGSN
DTTMKVWDLATGKLKTTLAGHVMTVRDVAVSDRHPYLFSVSEDKTVKCWDLEKNQIIRDYYGHLSGVRTVSIHPTLDLIA
TAGRDSVIKLWDMRTRIPVITLVGHKGPINQVQCTPVDPQVVSSSTDATVRLWDVVAGKTMKVLTHHKRSVRATALHPKE
FSVASACTDDIRSWGLAEGSLLTNFESEKTGIINTLSINQDDVLFAGGDNGVLSFYDYKSGHKYQSLATREMVGSLEGER
SVLCSTFDKTGLRLITGEADKSIKIWKQDETATKESEPGLAWNPNLSAKRF
Q12417
Pre-mRNA-processing protein 45
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.042548726999999995
1
LEVO
MFSNRLPPPKHSQGRVSTALSSDRVEPAILTDQIAKNVKLDDFIPKRQSNFELSVPLPTKAEIQECTARTKSYIQRLVNA
KLANSNNRASSRYVTETHQAPANLLLNNSHHIEVVSKQMDPLLPRFVGKKARKVVAPTENDEVVPVLHMDGSNDRGEADP
NEWKIPAAVSNWKNPNGYTVALERRVGKALDNENNTINDGFMKLSEALENADKKARQEIRSKMELKRLAMEQEMLAKESK
LKELSQRARYHNGTPQTGAIVKPKKQTSTVARLKELAYSQGRDVSEKIILGAAKRSEQPDLQYDSRFFTRGANASAKRHE
DQVYDNPLFVQQDIESIYKTNYEKLDEAVNVKSEGASGSHGPIQFTKAESDDKSDNYGA
P28004
Pre-mRNA-splicing factor SLT11
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.04098858999999999
1
LEVO
MNDEINEPPPNICEQCLGDEANIRMTKIPQGSECKICTLPFTLYHFKTSKRSNNIIKTLICVRCATQRNICQCCMLDSRW
HIPIQLRDHLISLVNEENVMTEEAKNDMMKRFLSLKNVKLGGAQITSDPSEADNIVDKLKNILLRATSDGPSTPLIKNTT
ALYKNEKGANEVKNLEKYASVDISHILKKLPLNESFLKNPSTKSFFLYNIDASIPEWKITDTVSQLLGIKKWKDGNSLSL
IVNHKAKCGGLRFQSSELGERFVSKISETLVTPKGLKRGVLLIDRFRIFIIPWSSGFSAASFGTNTAENIKLSLSLNKLI
QLELGLSFPTKSTDNAKNDKKKTSKKVHKDRSKKSKPRANKLTI
P38241
Pre-mRNA-splicing factor CWC2
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.038486561999999995
1
LEVO
MTSWRDKSAKVQVKESELPSSIPAQTGLTFNIWYNKWSQGFAGNTRFVSPFALQPQLHSGKTRGDNDGQLFFCLFFAKGM
CCLGPKCEYLHHIPDEEDIGKLALRTEVLDCFGREKFADYREDMGGIGSFRKKNKTLYVGGIDGALNSKHLKPAQIESRI
RFVFSRLGDIDRIRYVESKNCGFVKFKYQANAEFAKEAMSNQTLLLPSDKEWDDRREGTGLLVKWANEDPDPAAQKRLQE
ELKLESLNMMVHLINNNTNSAGTEVNNKNNERLDRTFPEASVDNVKKRLLPLDNGMESDDFIEKLKKVKKNISRENISSK
PSVGKLGGPLLDYLSSDED
Q12046
Pre-mRNA-splicing factor CWC15
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.019975194999999998
1
LEVO
MTTSHRPQLEARSGAKAAAYTPTGIEHARLLPGHTTLKYRKFKEEENLRANCAQEDRSNDKSLEEAVMNEEKQDVVGSGN
LQETRSEKDQKDSLQELLVTQKNKVEDKAELEGNEQLKGGNSSRRSWRKGTAFGRHKVTKETNIKEHATKKSASGYINDM
TKSEYHQEFLHKHVR
Q03772
Pre-mRNA-splicing factor BUD31
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.018484502
1
LEVO
MPRIKTRRSKPAPDGFEKIKPTLTDFEIQLRDAQKDKSSKLAAKSNEQLWEIMQLHHQRSRYIYTLYYKRKAISKDLYDW
LIKEKYADKLLIAKWRKTGYEKLCCLRCIQKNETNNGSTCICRVPRAQLEEEARKKGTQVSFHQCVHCGCRGCASTD
P25337
Pre-mRNA-splicing factor CWC22
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.067386062
1
LEVO
MSTATIQDEDIKFQRENWEMIRSHVSPIISNLTMDNLQESHRDLFQVNILIGRNIICKNVVDFTLNKQNGRLIPALSALI
ALLNSDIPDIGETLAKELMLMFVQQFNRKDYVSCGNILQCLSILFLYDVIHEIVILQILLLLLEKNSLRLVIAVMKICGW
KLALVSKKTHDMIWEKLRYILQTQELSSTLRESLETLFEIRQKDYKSGSQGLFILDPTSYTVHTHSYIVSDEDEANKELG
NFEKCENFNELTMAFDTLRQKLLINNTSDTNEGSNSQLQIYDMTSTNDVEFKKKIYLVLKSSLSGDEAAHKLLKLKIANN
LKKSVVDIIIKSSLQESTFSKFYSILSERMITFHRSWQTAYNETFEQNYTQDIEDYETDQLRILGKFWGHLISYEFLPMD
CLKIIKLTEEESCPQGRIFIKFLFQELVNELGLDELQLRLNSSKLDGMFPLEGDAEHIRYSINFFTAIGLGLLTEDMRSR
LTIIQEVEDAEEEEKKLREEEELEKLRKKARESQPTQGPKIHESRLFLQKDTRENSRSRSPFTVETRKRARSRTPPRGSR
NHRNRSRTPPARRQRHR
P53333
Pre-mRNA-splicing factor CEF1
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.067837773
1
LEVO
MPPVPIYVKGGVWTNVEDQILKAAVQKYGTHQWSKVASLLQKKTARQSELRWNEYLNPKLNFTEFSKEEDAQLLDLAREL
PNQWRTIADMMARPAQVCVERYNRLLESEDSGGAALSTGVTDLKAGDINPNAETQMARPDNGDLEDEEKEMLAEARARLL
NTQGKKATRKIRERMLEESKRIAELQKRRELKQAGINVAIKKPKKKYGTDIDYNEDIVYEQAPMPGIYDTSTEDRQIKKK
FEQFERKVNRKGLDGNKDKPSKKNKDKKRKHDENEHVEKAALGESTTLTDEYKKPKLILSAPGTKQGKVTYKKKLESKRQ
KLIEAQATGTVLTPKELLPHDSGQEDNERSNIKSGKQLKSRIRKFLVQMFASLPSPKNDFEIVLSEDEKEEDAEIAEYEK
EFENERAMNEEDNFIEPPSQNDAPRVSLVAVPLAYSTLPIPEFKNNPQSAIDNKYNLLVANAINKEPHMVPEDTVDFLKE
VESRMQHITQGRTSMKIQFKTAMPPTEVLLESIQSKVESIEQLQRKLQHVQPLEQQNNEMCSTLCHHSLPALIEGQRKYY
ADYYAYRQEIRSLEGRRKRLQAMLNSSSSI
Q03654
Pre-mRNA-splicing factor CLF1
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.082555859
1
LEVO
MDTLEPTAVDTHVSAEQILRDVYKKGQKARGSTNIDILDLEELREYQRRKRTEYEGYLKRNRLDMGQWIRYAQFEIEQHD
MRRARSIFERALLVDSSFIPLWIRYIDAELKVKCINHARNLMNRAISTLPRVDKLWYKYLIVEESLNNVEIVRSLYTKWC
SLEPGVNAWNSFVDFEIRQKNWNGVREIYSKYVMAHPQMQTWLKWVRFENRHGNTEFTRSVYSLAIDTVANLQNLQIWSD
MEVAKLVNSFAHWEAAQQEYERSSALYQIAIEKWPSNQLLKAGLLDFEKQFGDINSIEETISYKRKMEYETILSNNAYDY
DTWWLYLDLISESFPKQIMQTFEKAIVDSRPKELSKNVQWKRYIYLWMRYICYVELELENSLLEEELFQRLIDDIIPHKH
FTFSKIWLMYAKFLIRHDDVPKARKILGKAIGLCPKAKTFKGYIELEVKLKEFDRVRKIYEKFIEFQPSDLQIWSQYGEL
EENLGDWDRVRGIYTIALDENSDFLTKEAKIVLLQKYITFETESQEFEKARKLYRRYLELNQYSPQSWIEFAMYQTSTPT
EQQLLDLAKLQSENVDEDIEFEITDENKLEARKVFEEAIVFFKEKDDKQGRLSILEALKDYEETYGTELDQETVKKRFPK
VIKKVRLQNGVEEEFVDYIFPDDIDDDKPKPSKFLELAKKWKQEQAL
Q12309
Pre-mRNA-splicing factor SYF2
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.024850719
1
LEVO
MDFYKLDEKLKELKRKRVDVSIKSRKLADREIQEVSANRKPRVYSMEDVNDADESVGDTESPEKEKAFHYTVQEYDAWER
RHPQGKTGQSQRGGISYDQLAKLSYEKTLRNLATQTQNSSKQDSSADEEDNKNVPKKGRIGKVQKDTKTGKITIADDDKL
VNKLAVSLQSESKKRYEARKRQMQNAKTLYGVESFINDKNKQFNEKLSRESKGSE
P53277
Pre-mRNA-processing factor 17
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.052208738
1
LEVO
MGLVDGYDTSSDSDLNFDEGKSVHEKKNGNLHEDTSYEPSSNNIHKRKSHFTKSELKRRRKTRKGDGPWGSW(SEP)SSD
DETSQASETQKEDQDIFVHALAEDNLDSEQIEVEEVSHFYGKSEKDYQGRGYLYPPNDVDVDLREERISFRCYLPKKVIR
NYPGHPEGTTALKFLPKTGHLILSGGNDHTIKIWDFYHDYECLRDFQGHNKPIKALRFTEDCQSFLSSSFDRSVKIWDTE
TGKVKTRLHLNSTPADVESRPTNPHEFIVGLSNSKILHYDDRVSENQGLVQTYDHHLSSILALKYFPDGSKFISSSEDKT
VRIWENQINVPIKQISDTAQHSMPFLNVHPSQNYFCAQSMDNRIYSFSLKPKYKRHPKKIFKGHSSAGYGISLAFSGDGR
YICSGDSKSRLFTWDWNTSRLLNNIKIPGNKPITQVDWHPQETSKVICSGAAGKIYVCD
P40968
Pre-mRNA-splicing factor ISY1
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.028073835999999998
1
LEVO
MSRNVDKANSVLVRFQEQQAESAGGYKDYSRYQRPRNVSKVKSIKEANEWKRQVSKEIKQKSTRIYDPSLNEMQIAELND
ELNNLFKEWKRWQWHIDHTLMEKKTKRKRLEDSHVLMNSGKLINGKRYFGRALELPEVKEWLKQSQRQNDGGSINTKCIP
KDRNDFYYHGKVTAALTEFEANWTSILKAHYNVPVNEDEEEMSRQTQEIHVPTLADMEHWLVQRRKKKLMDELNL
P21374
ACT1 pre-mRNA
Saccharomyces cerevisiae S288c
S288c
0.217099734
1
GAGAGAUUCCGUACACCAUCAGGGUACGAGCUAGCCCAUGGCGUACACCAUCAGGGUACGACUAGUAGAUCUCGUACACC
AUCAGGGUACGGAAUUCUCUAGAGUGUCGACGGAUCCCCCUUUUAGAUUUUUCACGCUUACUGCUUUUUUCUUCCCAAGA
UCGAAAAUUUACUGAAUUAACAAUGGAUUCUGGUAUGUUCUAGCGCUUGCACCAUCCCAUUUAACUGUAAGAAGAAUUGC
ACGGUCCCAAUUGCUCGAGAGAUUUCUCUUUUACCUUUUUUUACUAUUUUUCACUCUCCCAUAACCUCCUAUAUUGACUG
AUCUGUAAUAACCACGAUAUUAUUGGAAUAAAUAGGGGCUUGAAAUUUGGAAAAAAAAAAAAAACUGAAAUAUUUUCGUG
AUAAGUGAUAGUGAUAUUCUUCUUUUAUUUGCUACUGUUACUAAGUCUCAUGUACUAACAUCGAUUGCUUCAUUCUUUUU
GUUGCUAUAUUAUAUGUUUAGAGGUUGCUGCUUUGGUUAUUGAUAACGGUUCUGGUAUGUGUAAAGCCGGUUUUGCCGGU
GACGACGCUCCUCGUGCUGUCUUCCCAUCUAUCGUCGGUAGACCAAGACACCAAGGUAUCAUGGUCGGUAUGGGUCAAAA
AGACUCCUACGUUGGUGAUGAAGCUCAAUCCAAGAGAGG
U5 snRNA
Saccharomyces cerevisiae S288c
S288c
0.068643344
1
AAGCAGCUUUACAGAUCAAUGGCGGAGGGAGGUCAACAUCAAGAACUGUGGGCCUUUUAUUGCCUAUAGAACUUAUAACG
AACAUGGUUCUUGCCUUUUACCAGAACCAUCCGGGUGUUGUCUCCAUAGAAACAGGUAAAGCUGUCCGUUACUGUGGGCU
UGCCAUAUUUUUUGGAACUUUUCUGCCCUUUUUCUCAAUGAGUAAGGAGGGCGU
U6 snRNA
Saccharomyces cerevisiae S288c
0.035883175999999996
1
GUUCGCGAAGUAACCCUUCGUGGACAUUUGGUCAAUUUGAAACAAUACAGAGAUGAUCAGCAGUUCCCCUGCAUAAGGAU
GAACCGUUUUACAAAGAGAUUUAUUUCGUUUU
CP007992.1
U2 snRNA
Saccharomyces cerevisiae S288c
S288c
0.376267406
1
ACGAAUCUCUUUGCCUUUUGGCUUAGAUCAAGUGUAGUAUCUGUUCUUUUCAGUGUAACAACUGAAAUGACCUCAAUGAG
GCUCAUUACCUUUUAAUUUGUUACAAUACACAUUUUUUGGCACCCAAAAUAAUAAAAUGGACGGGAAGAGACUUUUUAAG
CAAGUUGUUUUCCGCUAAUGUCAGGUCUCACUACUUUUUGCUGCUAUUUUUCUUCGCUCAUGGUUUCUUCAUAAGGCGUU
UUUAUGAUGGUUUUUCGAAAUUGGUUUUUGAGACGACGGUUGCUCAAGGUUAUUGUUUUUGUUUUCUUCUGGUUGUUUUC
UAUUUUCUUUUUUUUAGCUUUCUGUUUCUCCCUUAGUUUGGCUUUUUGCUUCAUACUCUUCCCUGUCUUUCCGAGCCGUU
UAUGUCCAACGCGGGAUUUGGUUUUUCUUUAUCGAUGGGAAGAAAUGGUGCUAUAGUAGGUUGGGAGAUAAUAUUUAUGG
UAUGGGGUGCUAGUGCGGAUGGGGCGCUCUUAUUGUUGAUUUCUUCGCUCGUCUUCUUUUUCUGGUGGCGCUGCAAGAGG
AAGUUUUUCGACUUUGUUAUGAUUUUUGGUUUGCAAGGAAAGGUGUCUUACGAUUCUUUUUUUGAUGUAAUAGGAUAAGC
UUGCUUAUCCCCCAAGUAUCGGCCAAAGUUGUUGAUUUUCCUUUUGAAGUGUCCUCGGUUUGAGGGGGUGUAGGGUGGGG
UUGGUCUACAAUAAGAGUGUUCCAUUGUUAACGUGCUGGCGUCUUUUACUAUAUUUUUUUUCCCAGUUUAUUUUGUGCUU
AUUUUCUCAUUGAGGAGAAGGAGCUCUUCUCGCAGGAUAUAAAUGGAGGUUUGCUAAAGGGGAGGAGAUGUGUUUGUGAG
AAUACUGCUGAGAGAGUUCUGGAAGAGAAAAAAAGGAGGCAAUGGAAGGCGUUUGCUGGGAAAAGAGAAGAGCCAUGACU
GCAUCUGUUGUUUCAAGGCCAGUUUUAUUAACCGCCUAUGUCAUAGAGGCGUUUUUUUUGGAGGGAUUUGAAGAAUGCCG
GCGGCAUCAAGAAACGGACUUGAUGGUUGACGCCUGUUUUUAAAGUUAGAGACGUCGCGACCCUCGCACUUGUGGAGUCG
UUCUUGACUUUUACUUUGGUCGCUUGAUGUUUCUCUCGUCUUCCCGUUCGCUCUU
Pre-mRNA-splicing factor SYF1
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.100344016
1
LEVO
MSAYIAMKGVITNVDENIRNDEDVAFEYEIQKTPQNILTWKRYIEYWKEEGRTDKQIRWLYERFCSQFVTDTSIWEDYIR
WESTKEVVETSRIFWLFQRCLKSCVRDCDRICLSYLELAIEQYDLAMIRHALASSLMKMEREMHRKVWDPVIKFVEEKVL
PLTQLDSTQEDEEESTDEAELINVLLVKGFTKGGFISEEISENGSRGDIWSSHILERYLKVAPQQKRNESLATLALTRDN
ITIKSVYEKYLPQDENSGKYLPSSELPFELNFNYLASLEKLGLDNQYEEFMRQMNGIYPDKWLFLILSLAKYYISRGRLD
SCGDLLKKSLQQTLRYSDFDRIYNFYLLFEQECSQFILGKLKENDSKFFNQKDWTEKLQAHMATFESLINLYDIYLNDVA
LRQDSNLVETWMKRVSLQKSAAEKCNVYSEAILKIDPRKVGTPGSFGRLWCSYGDLYWRSNAISTARELWTQSLKVPYPY
IEDLEEIYLNWADRELDKEGVERAFSILEDALHVPTNPEILLEKYKNGHRKIPAQTVLFNSLRIWSKYIDYLEAYCPKDA
NSSDKIFNKTKMAYNTVIDLRLITPAMAENFALFLQNHYEVMESFQVYEKTIPLFPPEIQYELWIEYLEVATSHQLSSLS
PEHIRFLFEKALKNLCSNGIDCKTIFIAYSVFEERISGLISKSIEILRRGAVIGTVSVSTHLESRLQLWRMCISKAESTL
GPSVTRELYQECIQILPNSKAVEFVIKFSDFESSIGETIRAREILAYGAKLLPPSRNTELWDSFEIFELKHGDKETYKDM
LKMKKVLESNMLIDSASVSHEEGNINFVAAATSHAPNSHTLTQSTSSYSINPDEIELDI
Q04048
U2 small nuclear ribonucleoprotein B''
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.012850944
1
LEVO
MVEPARKKQRIDRDTHHTVAEPVTEAKNTLYVSQLNEKINMQRLRVNLFLLFATFGEVLKVSMNFKKQRGQAFITMRTID
QASLAQISLNGERFFGKPLKVEFSKSETKTL
P40567
U2 small nuclear ribonucleoprotein A'
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.027232252
1
LEVO
MKFTPSIVIDAPQYYVDHFNGKYNVDKCVILRDLQLETDSESMPSSLKHLTKPTHILDLTNNDLIMIPDLSRRDDIHTLL
LGRNNIVEVDGRLLPMNVQNLTLSNNSIRRFEDLQRLRRAPRTLKNLTLIGNQVCHLANYREHVLRLVPHLETLDFQNVT
AEERKSAMSFPRQADGDTLGPVNTAIRDNGSRDKTMEIMNLVVSKMTVERRNELKKQLAEATSLEEIARLEKLLSGGV
Q08963
Pre-mRNA-splicing factor SNT309
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.020741455000000002
1
LEVO
MDGLSFVDKGKIPDGYKNEIDQLVKKEFANIKREPVHPEIRGILAKRKGADNSVSTLTNALYTEYLKQRNNKKRRTPDFN
DDDDTLFLEEYRRKYPRIDTSRYIPNESSEVSLLGIVDSYLKHQEIVLDTLLPQTVSNQWRINNDYIRQTCTIVEEMNIQ
QRKQINDLEIYRKRL
Q06091
Small nuclear ribonucleoprotein E
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.010385097999999999
2
LEVO
MSNKVKTKAMVPPINCIFNFLQQQTPVTIWLFEQIGIRIKGKIVGFDEFMNVVIDEAVEIPVNSADGKEDVEKGTPLGKI
LLKGDNITLITSAD
Q12330
Small nuclear ribonucleoprotein Sm D1
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.016296798
2
LEVO
MKLVNFLKKLRNEQVTIELKNGTTVWGTLQSVSPQMNAILTDVKLTLPQPRLNKLNSNGIAMASLYLTGGQQPTASDNIA
SLQYINIRGNTIRQIILPDSLNLDSLLVDQKQLNSLRRSGQIANDPSKKRRRDFGAPANKRPRRGL
Q02260
Small nuclear ribonucleoprotein G
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.008490808999999998
2
LEVO
MVSTPELKKYMDKKILLNINGSRKVAGILRGYDIFLNVVLDDAMEINGEDPANNHQLGLQTVIRGNSIISLEALDAI
P40204
Small nuclear ribonucleoprotein F
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.009669945
2
LEVO
MSESSDISAMQPVNPKPFLKGLVNHRVGVKLKFNSTEYRGTLVSTDNYFNLQLNEAEEFVAGVSHGTLGEIFIRCNNVLY
IRELPN
P54999
Small nuclear ribonucleoprotein Sm D2
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.012876066
2
LEVO
MSSQIIDRPKHELSRAELEELEEFEFKHGPMSLINDAMVTRTPVIISLRNNHKIIARVKAFDRHCNMVLENVKELWTEKK
GKNVINRERFISKLFLRGDSVIVVLKTPVE
Q06217
Small nuclear ribonucleoprotein-associated protein B
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.02242699
2
LEVO
MSKIQVAHSSRLANLIDYKLRVLTQDGRVYIGQLMAFDKHMNLVLNECIEERVPKTQLDKLRPRKDSKDGTTLNIKVEKR
VLGLTILRGEQILSTVVEDKPLLSKKERLVRDKKEKKQAQKQTKLRKEKEKKPGKIAKPNTANAKHTSSNSREIAQPSSS
RYNGGNDNIGANRSRFNNEAPPQTRKFQPPPGFKRK
P40018
Small nuclear ribonucleoprotein Sm D3
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.011240138999999998
2
LEVO
MTMNGIPVKLLNEAQGHIVSLELTTGATYRGKLVESEDSMNVQLRDVIATEPQGAVTHMDQIFVRGSQIKFIVVPDLLKN
APLFKKNSSRPMPPIRGPKRR
P43321
Pre-mRNA-processing factor 19
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ATCC 204508 / S288c
0.056629777
4
LEVO
MLCAISGKVPRRPVLSPKSRTIFEKSLLEQYVKDTGNDPITNEPLSIEEIVEIVPSAQQASLTESTNSATLKANYSIPNL
LTSLQNEWDAIMLENFKLRSTLDSLTKKLSTVMYERDAAKLVAAQLLMEKNEDSKDLPKSSQQAVAITREEFLQGLLQSS
RDFVARGKLKAPKWPILKNLELLQAQNYSRNIKTFPYKELNKSMYYDKWVCMCRCEDGALHFTQLKDSKTITTITTPNPR
TGGEHPAIISRGPCNRLLLLYPGNQITILDSKTNKVLREIEVDSANEIIYMYGHNEVNTEYFIWADNRGTIGFQSYEDDS
QYIVHSAKSDVEYSSGVLHKDSLLLALYSPDGILDVYNLSSPDQASSRFPVDEEAKIKEVKFADNGYWMVVECDQTVVCF
DLRKDVGTLAYPTYTIPEFKTGTVTYDIDDSGKNMIAYSNESNSLTIYKFDKKTKNWTKDEESALCLQSDTADFTDMDVV
CGDGGIAAILKTNDSFNIVALTP
P32523
2.3.2.27
INOSITOL HEXAKISPHOSPHATE
0.0006600349999999999
1
IHP
GUANOSINE-5'-TRIPHOSPHATE
0.0005231799999999999
1
GTP
MAGNESIUM ION
2.4305e-05
6
MG
ZINC ION
6.5409e-05
6
ZN
singleParticle
particle
0.2
7.9
Quantifoil R1.2/1.3
GOLD
300
CARBON
CONTINUOUS
GLOW DISCHARGE
ETHANE
FEI TITAN KRIOS
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
300
OTHER
NITROGEN
GATAN K2 SUMMIT (4k x 4k)
SUPER-RESOLUTION
49.3
1
C1
3.2
FSC 0.143 CUT-OFF
RELION
3.0
555036
PROJECTION MATCHING
RELION
3.0
MAXIMUM LIKELIHOOD
RELION
3.0
RELION
3.0