2020-12-02 REL RCSB RCSB RCSB 2019-02-21 2019-02-13 2019-03-13 2020-12-02 National Institutes of Health/National Institute of General Medical Sciences R01-GM63072 United States Swiss National Science Foundation P300PA_160983 Switzerland National Institutes of Health/National Institute of General Medical Sciences P01-GM063210 United States Swiss National Science Foundation P300PA_174355 Switzerland Cryo-EM structure of the human TFIIH core complex: Multibody-refined map, body 2 Greber BJ Toso D Fang J Nogales E Greber BJ Toso DB Fang J Nogales E The complete structure of the human TFIIH core complex. Elife US 8 2019 30860024 doi:10.7554/eLife.44771 2050-084X EMD-0452 other EM volume EMD-0603 associated EM volume EMD-0602 other EM volume EMD-0604 other EM volume Transcription factor IIH (TFIIH) Transcription factor IIH (TFIIH) 0 1 2 3 4 5 6 7 8 Homo sapiens 0.5 singleParticle particle 0.0049 7.9 20.0 Hepes-KOH 150.0 Sodium chloride 5.0 Magnesium chloride 0.015 NP40 substitute 1.5 Glycerol 2.0 Trehalose ETHANE 100 277 FEI VITROBOT MARK IV
A thin film of continuous carbon was floated onto Protochips C-flat CF-4/2 holey carbon grids and glow discharged or plasma cleaned before application of 4 uL of sample solution..
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.5 0.7 43478.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN VOLTA PHASE PLATE GIF Quantum LS 20
Residual beam tilt corrected in RELION 3.
GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 7 21437 8.25 50.0
Images were collected as dose-fractionated movie frames (33 or 50 frames per exposure).
 1 Gctf CTF determination RELION 3 CTF correction
CTF correction during 3D reconstruction in RELION 3.
 EMD-3802 1 C1 FOURIER SPACE 3.6 FSC 0.143 CUT-OFF RELION 3
Multibody-refined in RELION 3.
138659 MAXIMUM LIKELIHOOD RELION 3 MAXIMUM LIKELIHOOD RELION 3 8 100000 RELION 3 emd_0603.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 294.4 294.4 294.4 90.0 90.0 90.0 X Y Z -0.10053686 0.20372707 -0.0000213628 0.0031070653 1.15 1.15 1.15 0.0243 AUTHOR Multibody-refined cryo-EM map, body 2 (post-processed). 6NMI RIGID BODY FIT
This map supported model re-building in O and COOT.
REAL emd_0603_msk_1.map emd_0603_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 294.4 294.4 294.4 90.0 90.0 90.0 X Y Z -0.023343291 0.08368132 -0.00002000335 0.0015784134 1.15 1.15 1.15 AUTHOR Unfiltered half-map. emd_0603_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 294.4 294.4 294.4 90.0 90.0 90.0 X Y Z -0.022527752 0.09605159 -0.00002272215 0.0015782636 1.15 1.15 1.15 AUTHOR Unfiltered half-map.