2019-06-19 REL RCSB RCSB RCSB 2019-02-21 2019-03-20 2019-03-20 2019-06-19 National Institutes of Health/National Human Genome Research Institute R01-GM065050 United States Structure of a drug-like molecule stalled ribosome nascent chain complex (PCSK9-RNC) in rotated state with AA tRAN and PE tRNA Li W Cate JHD Li W Ward FR McClure KF Chang ST Montabana E Liras S Dullea RG Cate JHD Structural basis for selective stalling of human ribosome nascent chain complexes by a drug-like molecule. Nat.Struct.Mol.Biol. US 26 501 509 2019 31160784 doi:10.1038/s41594-019-0236-8 1545-9985 EMD-0597 associated EM volume EMD-0598 other EM volume EMD-0596 other EM volume EMD-0599 other EM volume EMD-0600 other EM volume EMD-0601 other EM volume 6om7 FULLOVERLAP Drug-like molecule PF846 stalled human ribosome nascent chain complex, PCSK9-RNC in rotated state with AA tRNA and PE tRNA Drug-like molecule PF846 stalled human ribosome nascent chain complex, PCSK9-RNC in rotated state with AA tRNA and PE tRNA 0 1 Homo sapiens Homo sapiens HeLa 4.3 singleParticle particle 7.8
unspecified
 ETHANE 100 4
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 SUMMIT (4k x 4k) 50.0 1 3.72 FSC 0.143 CUT-OFF 9564 ANGULAR RECONSTITUTION ANGULAR RECONSTITUTION emd_0597.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 430 430 430 0 0 0 430 430 430 524.60004 524.60004 524.60004 90.0 90.0 90.0 X Y Z -0.06245838 0.11255686 0.00044033065 0.006970685 1.22 1.22 1.22 0.022 AUTHOR PF846 stalled human ribosome nascent chain complex (PCSK9-RNC) in rotated state with AA tRNA and PE tRNA; map is sharpened. REAL 91.0 emd_0597_additional.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 430 430 430 0 0 0 430 430 430 524.60004 524.60004 524.60004 90.0 90.0 90.0 X Y Z -0.018077439 0.046085056 0.00046038718 0.003027126 1.22 1.22 1.22 AUTHOR PF846 stalled human ribosome nascent chain complex (PCSK9-RNC) in rotated state with AA tRNA and PE tRNA, map is not sharpened. emd_0597_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 430 430 430 0 0 0 430 430 430 524.60004 524.60004 524.60004 90.0 90.0 90.0 X Y Z -0.07964172 0.110560566 0.00044946137 0.01013388 1.22 1.22 1.22 EMDB Half map of PF846 stalled human ribosome nascent chain complex (PCSK9-RNC) in rotated state with AA tRNA and PE tRNA. emd_0597_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 430 430 430 0 0 0 430 430 430 524.60004 524.60004 524.60004 90.0 90.0 90.0 X Y Z -0.07481344 0.10960023 0.00043119997 0.009937811 1.22 1.22 1.22 EMDB Half map of PF846 stalled human ribosome nascent chain complex (PCSK9-RNC) in rotated state with AA tRNA and PE tRNA.