2019-12-04 REL RCSB RCSB RCSB 2019-02-13 2019-02-20 2019-11-06 2019-12-04 National Institutes of Health/National Institute of General Medical Sciences 2R01GM111730-05 United States Cryo-EM Map of the active Ragulator-RagA-RagC Complex Yokom AL Fromm SA Hurley JH Lawrence RE Fromm SA Fu Y Yokom AL Kim DJ Thelen AM Young LN Lim CY Samelson AJ Hurley JH Zoncu R Structural mechanism of a Rag GTPase activation checkpoint by the lysosomal folliculin complex. Science US 366 971 977 2019 31672913 doi:10.1126/science.aax0364 1095-9203 0038 SCIEAS EMD-0554 other EM volume EMD-7072 other EM volume EMD-0556 associated EM volume active RagA-RagC-Ragulator Complex active RagA-RagC-Ragulator Complex 0 1 2 3 4 5 6 7 8 9
RagA bound to GTP; RagC bound to XDP RagC has T90N and D181N mutations
Homo sapiens Spodoptera frugiperda Sf9 0.15 singleParticle particle 0.5 7.4 25.0 HEPES 130.0 NaCl sodium chloride 2.5 MgCl2 magnesium chloride 0.5 TCEP C-flat-2/1 COPPER 400 CARBON HOLEY ETHANE 90 298 FEI VITROBOT MARK IV
FEI TITAN KRIOS OTHER BRIGHT FIELD FIELD EMISSION GUN 300 2.7 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 20 GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 8.0 48.0 1 Gctf
cryoSPARC v2 ab-initio
 C1 8.1 FSC 0.143 CUT-OFF cryoSPARC 2 75996 NOT APPLICABLE NOT APPLICABLE emd_0556.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 229.8 229.8 229.8 90.0 90.0 90.0 X Y Z -0.8477015 1.839886 0.0055222926 0.050337825 1.149 1.149 1.149 0.3 AUTHOR em-volume_P1