2024-03-20 REL RCSB RCSB RCSB 2019-01-10 2019-06-12 2019-06-12 2024-03-20 CryoEM structure of the LbCas12a-crRNA-2xAcrVA1 complex Chang L Li Z Unknown Function-RNA complex Zhang H Li Z Daczkowski CM Gabel C Mesecar AD Chang L Structural Basis for the Inhibition of CRISPR-Cas12a by Anti-CRISPR Proteins. Cell Host Microbe US 25 815 2019 31155345 doi:10.1016/j.chom.2019.05.004 1934-6069 6nmc FULLOVERLAP protein complex 3 protein complex 3 0 1 2 3 Lachnospiraceae bacterium ND2006 Cpf1 Lachnospiraceae bacterium ND2006 0.143750219 1 Escherichia coli LEVO MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAEDYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISL FRKKTRTEKENKELENLEINLRKEIAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNREN MFSEEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGEFFNFVLTQEGIDVYNAI IGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLSDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKK LEKLFKNFDEYSSAGIFVKNGPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDLLDSVKSFENYIKAFFGEGKET NRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSKDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDK KYAKCLQKIDKDDVNGNYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLIDFFK DSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVEEGKLYMFQIYNKDFSDKSHGTPNLH TMYFKLLFDENNHGQIRLSGGAELFMRRASLKKEELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPI AINKCPKNIFKINTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDYHSLLDKKE KERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGFKNSRVKVEKQVYQKFEKMLIDKLNYMVDK KSNPCATGGALKGYQITNKFESFKSMSTQNGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEE DLFEFALDYKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQGDIRALLCEQSD KAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNYEAQENAILPKNADANGAYNIARKVLWAIGQFKK AEDEKLDKVKIAISNKEWLEYAQTSVK A0A182DWE3 RNA Lachnospiraceae bacterium ND2006 0.012879634 1 AAUUUCUACUAAGUGUAGAUGGAAAUUAGGUGCGCUUGGC AcrVA1 Moraxella bovoculi 0.019650275000000002 2 Escherichia coli 'BL21-Gold(DE3)pLysS AG' LEVO SKAMYEAKERYAKKKMQENTKIDTLTDEQHDALAQLCAFRHKFHSNKDSLFLSESAFSGEFSFEMQSDENSKLREVGLPT IEWSFYDNSHIPDDSFREWFNFANYSELSETIQEQGLELDLDDDETYELVYDELYTEAMGEYEELNQDIEKYLRRIDEEH GTQYC MAGNESIUM ION 2.4305e-05 2 MG water 1.8015e-05 6 HOH singleParticle particle 1 7.5 ETHANE FEI TITAN KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 35.0 1 C1 4.24 FSC 0.143 CUT-OFF RELION 3.0b 28402 RANDOM ASSIGNMENT PROJECTION MATCHING emd_0446.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 168 168 168 0 0 0 168 168 168 179.088 179.088 179.088 90.0 90.0 90.0 X Y Z -0.070723824 0.14439781 0.00096183794 0.007162692 1.066 1.066 1.066 0.0169 AUTHOR LbCas12a-crRNA-2xAcrVA1