2019-03-06 REL RCSB RCSB RCSB 2018-12-09 2019-01-09 2019-02-27 2019-03-06 T.elongatus NDH Peripheral Arm Focus Map with X-cofactor(data-set 1) Laughlin TG Davies KM Laughlin TG Bayne AN Trempe JF Savage DF Davies KM Structure of the complex I-like molecule NDH of oxygenic photosynthesis. Nature UK 566 411 414 2019 30742075 doi:10.1038/s41586-019-0921-0 1476-4687 0006 NATUAS EMD-0415 other EM volume EMD-0416 other EM volume EMD-0417 other EM volume EMD-0418 other EM volume EMD-0419 associated EM volume NAD(P)H dehydrogenase-like complex peripheral arm(NDH/NDH-1_1/NDH1L) from T.elongatus NAD(P)H dehydrogenase-like complex peripheral arm(NDH/NDH-1_1/NDH1L) from T.elongatus 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 Thermosynechococcus elongatus BP-1 singleParticle particle 0.01 6.0 20.0 Bis-Tris 100.0 sodium chloride 0.03 B-dodecyl-maltoside ETHANE 100 295 FEI VITROBOT MARK IV
incubate on grid for 30 seconds and blot 2.5 seconds before plunging.
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 1-35 1 2519 0.2 50.0
One image per hole, focusing at each image.
 1
Super-resolution movies were aligned, exposure-weighted, and Fourier cropped to the physical pixel size.
176592 Gctf 1.06 RELION 2.1.1
Signal-subtraction of original map
 BACK PROJECTION 3.3 FSC 0.143 CUT-OFF RELION 3.0.b2 35662 MAXIMUM LIKELIHOOD RELION 2.1.1 MAXIMUM LIKELIHOOD RELION 2.1.1 emd_0419.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 384.47998 384.47998 384.47998 90.0 90.0 90.0 X Y Z -0.021727258 0.08977797 -0.00007326881 0.0011539819 1.068 1.068 1.068 0.0127 AUTHOR unsharpened emd_0419_msk_1.map emd_0419_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 384.12003 384.12003 384.12003 90.0 90.0 90.0 X Y Z -0.03943012 0.09366396 -0.0000664284 0.0025665446 1.067 1.067 1.067 AUTHOR em-half-volume_P2 emd_0419_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 384.12003 384.12003 384.12003 90.0 90.0 90.0 X Y Z -0.03599318 0.107844874 -0.00006694981 0.0025810895 1.067 1.067 1.067 AUTHOR em-half-volume_P1