2024-10-09 REL RCSB RCSB RCSB 2018-11-18 2019-01-23 2019-01-30 2024-10-09 National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health & Human Development (NIH/NICHD) R37DA036246 United States National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) R01GM083118 United States Krishna Kumar K Shalev-Benami M Cannabinoid receptor, GPCR, synthetic cannabinoid, Fubinaca, Gi, SIGNALING PROTEIN Krishna Kumar K Shalev-Benami M Robertson MJ Hu H Banister SD Hollingsworth SA Latorraca NR Kato HE Hilger D Maeda S Weis WI Farrens DL Dror RO Malhotra SV Kobilka BK Skiniotis G Cell 176 448 2019 30639101 doi:10.1016/j.cell.2018.11.040 1097-4172 6n4b FULLOVERLAP Cannabinoid Receptor 1-G Protein Complex Cannabinoid Receptor 1-G Protein Complex 0 1 2 3 4 5 Homo sapiens Guanine nucleotide-binding protein G(i) subunit alpha-1 Homo sapiens 0.040415031000000004 1 Trichoplusia ni LEVO MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQS IIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLD RIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIY THFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF P63096 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Homo sapiens 0.037671102 1 Trichoplusia ni LEVO PGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQD GKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQ IVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFP NGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVS CLGVTDDGMAVATGSWDSFLKIWN P62873 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Homo sapiens 0.007861143 1 Trichoplusia ni LEVO MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL P59768 Cannabinoid receptor 1 Homo sapiens 0.055678535 1 Spodoptera frugiperda LEVO DYKDDDDAMKSILDGLADTTFRTITTDLLYVGSNDIQYEDIKGDMASKLGYFPQKFPLTSFRGSPFQEKMTAGDNPQLVP ADQVNITEFYNKSLSENLYFQGSFKENEENIQCGENFMDIECFMVLNPSQQLAIAVLSLTLGTFTVLENLLVLCVILHSR SLRCRPSYHFIGSLAVADLLGSVIFVYSFIDFHVFHRKDSRNVFLFKLGGVTASFTASVGSLFLTAIDRYISIHRPLAYK RIVTRPKAVVAFCLMWTIAIVIAVLPLLGWNCEKLQSVCSDIFPHIDETYLMFWIGVTSVLLLFIVYAYMYILWKAHSHA VRMIQRGTQKSIIIHTSEDGKVQVTRPDQARMDIRLAKTLVLILVVLIICWGPLLAIMVYDVFGKMNKLIKTVFAFCSML CLLNSTVNPIIYALRSKDLRHAFRSMFPSCEGTAQPLDNSMGDSDCLHKHANNAASVHRAAESCIKSTVKIAKVTMSVST DTSAEALGSHHHHHH P21554 scFv16 Mus musculus 0.027784896 1 Trichoplusia ni LEVO DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLF LQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCR SSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG AGTKLELKAAAHHHHHHHH methyl N-{1-[(4-fluorophenyl)methyl]-1H-indazole-3-carbonyl}-3-methyl-L-valinate 0.000397443 1 KCA CHOLESTEROL 0.000386654 2 CLR singleParticle particle 5 7.5 Quantifoil R2/2 GOLD 200 GLOW DISCHARGE 80 ETHANE FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 SUMMIT (4k x 4k) COUNTING 50.0 1 C1 3.0 FSC 0.143 CUT-OFF 177787 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD