data_EMD-0322
# 
_entry.id   EMD-0322 
# 
_audit_conform.dict_name       mmcif_pdbx.dic 
_audit_conform.dict_version    5.397 
_audit_conform.dict_location   http://mmcif.pdb.org/dictionaries/ascii/mmcif_pdbx.dic 
# 
loop_
_database_2.database_id 
_database_2.database_code 
_database_2.pdbx_database_accession 
_database_2.pdbx_DOI 
PDB   6I0Y         pdb_00006i0y 10.2210/pdb6i0y/pdb 
WWPDB D_1200008610 ?            ?                   
EMDB  EMD-0322     ?            ?                   
# 
_pdbx_database_related.db_name        EMDB 
_pdbx_database_related.details        
'TnaC-stalled ribosome complex with the titin I27 domain folding close to the ribosomal exit tunnel' 
_pdbx_database_related.db_id          EMD-0322 
_pdbx_database_related.content_type   'associated EM volume' 
# 
_citation.abstract                  ? 
_citation.abstract_id_CAS           ? 
_citation.book_id_ISBN              ? 
_citation.book_publisher            ? 
_citation.book_publisher_city       ? 
_citation.book_title                ? 
_citation.coordinate_linkage        ? 
_citation.country                   US 
_citation.database_id_Medline       ? 
_citation.details                   ? 
_citation.id                        primary 
_citation.journal_abbrev            'Proc. Natl. Acad. Sci. U.S.A.' 
_citation.journal_id_ASTM           PNASA6 
_citation.journal_id_CSD            0040 
_citation.journal_id_ISSN           1091-6490 
_citation.journal_full              ? 
_citation.journal_issue             ? 
_citation.journal_volume            115 
_citation.language                  ? 
_citation.page_first                E11284 
_citation.page_last                 E11293 
_citation.title                     'Folding pathway of an Ig domain is conserved on and off the ribosome.' 
_citation.year                      2018 
_citation.database_id_CSD           ? 
_citation.pdbx_database_id_DOI      10.1073/pnas.1810523115 
_citation.pdbx_database_id_PubMed   30413621 
_citation.unpublished_flag          ? 
# 
loop_
_citation_author.citation_id 
_citation_author.name 
_citation_author.ordinal 
_citation_author.identifier_ORCID 
primary 'Tian, P.'       1  ?                   
primary 'Steward, A.'    2  ?                   
primary 'Kudva, R.'      3  0000-0003-0426-3716 
primary 'Su, T.'         4  ?                   
primary 'Shilling, P.J.' 5  0000-0001-5216-6975 
primary 'Nickson, A.A.'  6  ?                   
primary 'Hollins, J.J.'  7  ?                   
primary 'Beckmann, R.'   8  ?                   
primary 'von Heijne, G.' 9  0000-0002-4490-8569 
primary 'Clarke, J.'     10 ?                   
primary 'Best, R.B.'     11 0000-0002-7893-3543 
# 
loop_
_entity.id 
_entity.type 
_entity.src_method 
_entity.pdbx_description 
_entity.formula_weight 
_entity.pdbx_number_of_molecules 
_entity.pdbx_ec 
_entity.pdbx_mutation 
_entity.pdbx_fragment 
_entity.details 
1  polymer     nat '50S ribosomal protein L24'           11339.250  1   ?        ? ? ? 
2  polymer     nat '50S ribosomal protein L32'           6463.445   1   ?        ? ? ? 
3  polymer     nat '50S ribosomal protein L33'           6388.631   1   ?        ? ? ? 
4  polymer     nat '50S ribosomal protein L34'           5397.463   1   ?        ? ? ? 
5  polymer     nat '50S ribosomal protein L35'           7313.032   1   ?        ? ? ? 
6  polymer     nat '50S ribosomal protein L36'           4377.390   1   ?        ? ? ? 
7  polymer     nat '50S ribosomal protein L10'           17736.596  1   ?        ? ? ? 
8  polymer     nat '50S ribosomal protein L7/L12'        12309.155  1   ?        ? ? ? 
9  polymer     man 'Tryptophanase operon leader peptide' 2899.416   1   ?        ? ? ? 
10 polymer     nat '50S ribosomal protein L25'           10713.465  1   ?        ? ? ? 
11 polymer     nat '23S ribosomal RNA'                   941306.188 1   ?        ? ? ? 
12 polymer     nat '5S ribosomal RNA'                    38177.762  1   ?        ? ? ? 
13 polymer     nat '50S ribosomal protein L2'            29923.619  1   ?        ? ? ? 
14 polymer     nat '50S ribosomal protein L3'            22277.535  1   ?        ? ? ? 
15 polymer     nat '50S ribosomal protein L4'            22121.566  1   ?        ? ? ? 
16 polymer     nat '50S ribosomal protein L5'            20073.234  1   ?        ? ? ? 
17 polymer     nat '50S ribosomal protein L6'            18801.598  1   ?        ? ? ? 
18 polymer     nat '50S ribosomal protein L9'            5467.367   1   ?        ? ? ? 
19 polymer     nat '50S ribosomal protein L11'           14763.165  1   ?        ? ? ? 
20 polymer     nat '50S ribosomal protein L13'           16050.606  1   ?        ? ? ? 
21 polymer     nat '50S ribosomal protein L14'           13451.910  1   ?        ? ? ? 
22 polymer     nat '50S ribosomal protein L15'           14877.273  1   ?        ? ? ? 
23 polymer     nat '50S ribosomal protein L16'           15312.269  1   ?        ? ? ? 
24 polymer     nat '50S ribosomal protein L17'           13721.938  1   ?        ? ? ? 
25 polymer     nat '50S ribosomal protein L18'           12663.471  1   ?        ? ? ? 
26 polymer     nat '50S ribosomal protein L19'           13028.082  1   ?        ? ? ? 
27 polymer     nat '50S ribosomal protein L20'           13396.828  1   ?        ? ? ? 
28 polymer     nat '50S ribosomal protein L21'           11586.374  1   ?        ? ? ? 
29 polymer     nat '50S ribosomal protein L22'           12253.359  1   ?        ? ? ? 
30 polymer     nat '50S ribosomal protein L23'           10546.472  1   ?        ? ? ? 
31 polymer     nat 'Proline tRNA'                        24876.777  1   ?        ? ? ? 
32 polymer     nat '50S ribosomal protein L27'           8476.680   1   ?        ? ? ? 
33 polymer     nat '50S ribosomal protein L28'           8896.354   1   ?        ? ? ? 
34 polymer     nat '50S ribosomal protein L29'           7286.464   1   ?        ? ? ? 
35 polymer     nat '50S ribosomal protein L30'           6423.625   1   ?        ? ? ? 
36 polymer     man Titin                                 9794.193   1   2.7.11.1 ? ? ? 
37 non-polymer syn 'MAGNESIUM ION'                       24.305     143 ?        ? ? ? 
38 non-polymer syn 'ZINC ION'                            65.409     1   ?        ? ? ? 
39 non-polymer nat TRYPTOPHAN                            204.225    1   ?        ? ? ? 
40 water       nat water                                 18.015     439 ?        ? ? ? 
# 
loop_
_entity_poly.entity_id 
_entity_poly.type 
_entity_poly.pdbx_seq_one_letter_code 
1  'polypeptide(L)'   
;MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKKHQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKA
DRVGFRFEDGKKVRFFKSNSETIK
;
2  'polypeptide(L)'   MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHITADGYYRGRKVIAK 
3  'polypeptide(L)'   MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVRQHVIYKEAKIK 
4  'polypeptide(L)'   MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTVSK 
5  'polypeptide(L)'   MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHLRPKAMVSKGDLGLVIACLPYA 
6  'polypeptide(L)'   MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG 
7  'polypeptide(L)'   
;MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKAGREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPT
LIAYSMEHPGAAARLFKEFAKANAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
AKEAA
;
8  'polypeptide(L)'   
;MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAGPVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLG
LKEAKDLVESAPAALKEGVSKDDAEALKKALEEAGAEVEVK
;
9  'polypeptide(L)'   MNILHICVTSKWFNIDNKIVDHRP 
10 'polypeptide(L)'   
;MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDHDKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRH
PYKPKLQHIDFVRA
;
11 polyribonucleotide 
;GGUUAAGCGACUAAGCGUACACGGUGGAUGCCCUGGCAGUCAGAGGCGAUGAAGGACGUGCUAAUCUGCGAUAAGCGUCG
GUAAGGUGAUAUGAACCGUUAUAACCGGCGAUUUCCGAAUGGGGAAACCCAGUGUGUUUCGACACACUAUCAUUAACUGA
AUCCAUAGGUUAAUGAGGCGAACCGGGGGAACUGAAACAUCUAAGUACCCCGAGGAAAAGAAAUCAACCGAGAUUCCCCC
AGUAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCUGAAUCAGUGUGUGUGUUAGUGGAAGCGUCUGGAAAGGCGCGCGA
UACAGGGUGACAGCCCCGUACACAAAAAUGCACAUGCUGUGAGCUCGAUGAGUAGGGCGGGACACGUGGUAUCCUGUCUG
AAUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCCUGACUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAA
GAACCCCGGCGAGGGGAGUGAAAAAGAACCUGAAACCGUGUACGUACAAGCAGUGGGAGCACGCUUAGGCGUGUGACUGC
GUACCUUUUGUAUAAUGGGUCAGCGACUUAUAUUCUGUAGCAAGGUUAACCGAAUAGGGGAGCCGAAGGGAAACCGAGUC
UUAACUGGGCGUUAAGUUGCAGGGUAUAGACCCGAAACCCGGUGAUCUAGCCAUGGGCAGGUUGAAGGUUGGGUAACACU
AACUGGAGGACCGAACCGACUAAUGUUGAAAAAUUAGCGGAUGACUUGUGGCUGGGGGUGAAAGGCCAAUCAAACCGGGA
GAUAGCUGGUUCUCCCCGAAAGCUAUUUAGGUAGCGCCUCGUGAAUUCAUCUCCGGGGGUAGAGCACUGUUUCGGCAAGG
GGGUCAUCCCGACUUACCAACCCGAUGCAAACUGCGAAUACCGGAGAAUGUUAUCACGGGAGACACACGGCGGGUGCUAA
CGUCCGUCGUGAAGAGGGAAACAACCCAGACCGCCAGCUAAGGUCCCAAAGUCAUGGUUAAGUGGGAAACGAUGUGGGAA
GGCCCAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGGCCUG
CGCGGAAGAUGUAACGGGGCUAAACCAUGCACCGAAGCUGCGGCAGCGACGCUUAUGCGUUGUUGGGUAGGGGAGCGUUC
UGUAAGCCUGCGAAGGUGUGCUGUGAGGCAUGCUGGAGGUAUCAGAAGUGCGAAUGCUGACAUAAGUAACGAUAAAGCGG
GUGAAAAGCCCGCUCGCCGGAAGACCAAGGGUUCCUGUCCAACGUUAAUCGGGGCAGGGUGAGUCGACCCCUAAGGCGAG
GCCGAAAGGCGUAGUCGAUGGGAAACAGGUUAAUAUUCCUGUACUUGGUGUUACUGCGAAGGGGGGACGGAGAAGGCUAU
GUUGGCCGGGCGACGGUUGUCCCGGUUUAAGCGUGUAGGCUGGUUUUCCAGGCAAAUCCGGAAAAUCAAGGCUGAGGCGU
GAUGACGAGGCACUACGGUGCUGAAGCAACAAAUGCCCUGCUUCCAGGAAAAGCCUCUAAGCAUCAGGUAACAUCAAAUC
GUACCCCAAACCGACACAGGUGGUCAGGUAGAGAAUACCAAGGCGCUUGAGAGAACUCGGGUGAAGGAACUAGGCAAAAU
GGUGCCGUAACUUCGGGAGAAGGCACGCUGAUAUGUAGGUGAGGUCCCUCGCGGAUGGAGCUGAAAUCAGUCGAAGAUAC
CAGCUGGCUGCAACUGUUUAUUAAAAACACAGCACUGUGCAAACACGAAAGUGGACGUAUACGGUGUGACGCCUGCCCGG
UGCCGGAAGGUUAAUUGAUGGGGUUAGCGCAAGCGAAGCUCUUGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAAC
GGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCUGCACGAAUGGCGUAAUGAUGGCCAGGCUGUCUCCACC
CGAGACUCAGUGAAAUUGAACUCGCUGUGAAGAUGCAGUGUACCCGCGGCAAGACGGAAAGACCCCGUGAACCUUUACUA
UAGCUUGACACUGAACAUUGAGCCUUGAUGUGUAGGAUAGGUGGGAGGCUUUGAAGUGUGGACGCCAGUCUGCAUGGAGC
CGACCUUGAAAUACCACCCUUUAAUGUUUGAUGUUCUAACGUUGACCCGUAAUCCGGGUUGCGGACAGUGUCUGGUGGGU
AGUUUGACUGGGGCGGUCUCCUCCUAAAGAGUAACGGAGGAGCACGAAGGUUGGCUAAUCCUGGUCGGACAUCAGGAGGU
UAGUGCAAUGGCAUAAGCCAGCUUGACUGCGAGCGUGACGGCGCGAGCAGGUGCGAAAGCAGGUCAUAGUGAUCCGGUGG
UUCUGAAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCCGGGGAUAACAGGCUGAUACCGCCCAAGAGUUCAUAUC
GACGGCGGUGUUUGGCACCUCGAUGUCGGCUCAUCACAUCCUGGGGCUGAAGUAGGUCCCAAGGGUAUGGCUGUUCGCCA
UUUAAAGUGGUACGCGAGCUGGGUUUAGAACGUCGUGAGACAGUUCGGUCCCUAUCUGCCGUGGGCGCUGGAGAACUGAG
GGGGGCUGCUCCUAGUACGAGAGGACCGGAGUGGACGCAUCACUGGUGUUCGGGUUGUCAUGCCAAUGGCACUGCCCGGU
AGCUAAAUGCGGAAGAGAUAAGUGCUGAAAGCAUCUAAGCACGAAACUUGCCCCGAGAUGAGUUCUCCCUGACCCUUUAA
GGGUCCUGAAGGAACGUUGAAGACGACGACGUUGAUAGGCCGGGUGUGUAAGCGCAGCGAUGCGUUGAGCUAACCGGUAC
UAAUGAACCGUGAGGCUUAACCU
;
12 polyribonucleotide 
;GCCUGGCGGCCGUAGCGCGGUGGUCCCACCUGACCCCAUGCCGAACUCAGAAGUGAAACGCCGUAGCGCCGAUGGUAGUG
UGGGGUCUCCCCAUGCGAGAGUAGGGAACUGCCAGGCA
;
13 'polypeptide(L)'   
;MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRNNNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVER
LEYDPNRSANIALVLYKDGERRYILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRPTVRGTAMNPVDHPHGGGEGRNF
GKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK
;
14 'polypeptide(L)'   
;MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDGYRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLW
EFRLAEGEEFTVGQSISVELFADVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA
;
15 'polypeptide(L)'   
;MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTRAQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRS
GGVTFAARPQDHSQKVNKKMYRGALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA
;
16 'polypeptide(L)'   
;AKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQ
GYPIGCKVTLRGERMWEFFERLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTAK
SDEEGRALLAAFDFPFR
;
17 'polypeptide(L)'   
;SRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVKHADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTE
GFTKKLQLVGVGYRAAVKGNVINLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGKG
VRYADEVVRTKEAKKK
;
18 'polypeptide(L)'   MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNIEFFEAR 
19 'polypeptide(L)'   
;AKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNAKTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLK
KAAGIKSGSGKPNKDKVGKISRAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED
;
20 'polypeptide(L)'   
;MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEYTPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGH
IGGIKQATFEEMIARRPERVIEIAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI
;
21 'polypeptide(L)'   
;MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIKEAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFD
GNACVLLNNNSEQPIGTRIFGPVTRELRSEKFMKIISLAPEV
;
22 'polypeptide(L)'   
;RLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGGGVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEIRLSD
LAKVEGGVVDLNTLKAANIIGIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE
;
23 'polypeptide(L)'   
;MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTARQIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAV
RMGKGKGNVEYWVALIQPGKVLYEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM
;
24 'polypeptide(L)'   
;MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKELRRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLF
NELGPRFASRAGGYTRILKCGFRAGDNAPMAYIELVDRSE
;
25 'polypeptide(L)'   
;DKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNGSEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAER
ALEKGIKDVSFDRSGFQYHGRVQALADAAREAGLQF
;
26 'polypeptide(L)'   
;SNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFEGVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVV
DSISVKRRGAVRKAKLYYLRERTGKAARIKERLN
;
27 'polypeptide(L)'   
;ARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQYAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFIN
GLKKASVEIDRKILADIAVFDKVAFTALVEKAKAALA
;
28 'polypeptide(L)'   
;MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANGEEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRR
KHYRKQQGHRQWFTDVKITGISA
;
29 'polypeptide(L)'   
;METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAAVLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGP
SMKRIMPRAKGRADRILKRTSHITVVVSDR
;
30 'polypeptide(L)'   
;MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIKAAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDW
KKAYVTLKEGQNL
;
31 polyribonucleotide CGGCACGUAGCGCAGCCUGGUAGCGCACCGUCAUGGGGUGUCGGGGGUCGGAGGUUCAAAUCCUCUCGUGCCGACCA 
32 'polypeptide(L)'   GGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTKFHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE 
33 'polypeptide(L)'   SRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEKRFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY 
34 'polypeptide(L)'   MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLKQVRRDVARVKTLLNEKAGA 
35 'polypeptide(L)'   AKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAIRGMINAVSFMVKVEE 
36 'polypeptide(L)'   
;LIEVEKPLYGVEVFVGETAHFEIELSEPDVHGQWKLKGQPLTASPDCEIIEDGKKHILILHNCQLGMTGEVSFQAANAKS
AANLKVKEL
;
# 
loop_
_pdbx_entity_nonpoly.entity_id 
_pdbx_entity_nonpoly.name 
_pdbx_entity_nonpoly.comp_id 
37 'MAGNESIUM ION' MG  
38 'ZINC ION'      ZN  
39 TRYPTOPHAN      TRP 
40 water           HOH 
# 
loop_
_entity_src_gen.entity_id 
_entity_src_gen.pdbx_src_id 
_entity_src_gen.pdbx_alt_source_flag 
_entity_src_gen.pdbx_seq_type 
_entity_src_gen.pdbx_beg_seq_num 
_entity_src_gen.pdbx_end_seq_num 
_entity_src_gen.gene_src_common_name 
_entity_src_gen.gene_src_genus 
_entity_src_gen.pdbx_gene_src_gene 
_entity_src_gen.gene_src_species 
_entity_src_gen.gene_src_strain 
_entity_src_gen.gene_src_tissue 
_entity_src_gen.gene_src_tissue_fraction 
_entity_src_gen.gene_src_details 
_entity_src_gen.pdbx_gene_src_fragment 
_entity_src_gen.pdbx_gene_src_scientific_name 
_entity_src_gen.pdbx_gene_src_ncbi_taxonomy_id 
_entity_src_gen.pdbx_gene_src_variant 
_entity_src_gen.pdbx_gene_src_cell_line 
_entity_src_gen.pdbx_gene_src_atcc 
_entity_src_gen.pdbx_gene_src_culture_collection 
_entity_src_gen.pdbx_gene_src_organ 
_entity_src_gen.pdbx_gene_src_organelle 
_entity_src_gen.pdbx_gene_src_cell 
_entity_src_gen.pdbx_gene_src_cellular_location 
_entity_src_gen.host_org_common_name 
_entity_src_gen.pdbx_host_org_scientific_name 
_entity_src_gen.pdbx_host_org_ncbi_taxonomy_id 
_entity_src_gen.host_org_genus 
_entity_src_gen.pdbx_host_org_gene 
_entity_src_gen.pdbx_host_org_organ 
_entity_src_gen.host_org_species 
_entity_src_gen.pdbx_host_org_tissue 
_entity_src_gen.pdbx_host_org_tissue_fraction 
_entity_src_gen.pdbx_host_org_strain 
_entity_src_gen.pdbx_host_org_variant 
_entity_src_gen.pdbx_host_org_cell_line 
_entity_src_gen.pdbx_host_org_atcc 
_entity_src_gen.pdbx_host_org_culture_collection 
_entity_src_gen.pdbx_host_org_cell 
_entity_src_gen.pdbx_host_org_organelle 
_entity_src_gen.pdbx_host_org_cellular_location 
_entity_src_gen.pdbx_host_org_vector_type 
_entity_src_gen.pdbx_host_org_vector 
_entity_src_gen.host_org_details 
_entity_src_gen.expression_system_id 
_entity_src_gen.plasmid_name 
_entity_src_gen.plasmid_details 
_entity_src_gen.pdbx_description 
9  1 sample 'Biological sequence' 1 24 ?     ? 'tnaC, tnaL, b3707, JW3685' ? KC6 ? ? ? ? 'Escherichia coli' 562  ? ? ? ? ? ? ? ? ? 
'Escherichia coli' 562 ? ? ? ? ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
36 1 sample 'Biological sequence' 1 89 Human ? TTN                         ? ?   ? ? ? ? 'Homo sapiens'     9606 ? ? ? ? ? ? ? ? ? 
'Escherichia coli' 562 ? ? ? ? ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
# 
loop_
_entity_src_nat.entity_id 
_entity_src_nat.pdbx_src_id 
_entity_src_nat.pdbx_alt_source_flag 
_entity_src_nat.pdbx_beg_seq_num 
_entity_src_nat.pdbx_end_seq_num 
_entity_src_nat.common_name 
_entity_src_nat.pdbx_organism_scientific 
_entity_src_nat.pdbx_ncbi_taxonomy_id 
_entity_src_nat.genus 
_entity_src_nat.species 
_entity_src_nat.strain 
_entity_src_nat.tissue 
_entity_src_nat.tissue_fraction 
_entity_src_nat.pdbx_secretion 
_entity_src_nat.pdbx_fragment 
_entity_src_nat.pdbx_variant 
_entity_src_nat.pdbx_cell_line 
_entity_src_nat.pdbx_atcc 
_entity_src_nat.pdbx_culture_collection 
_entity_src_nat.pdbx_cellular_location 
_entity_src_nat.pdbx_organ 
_entity_src_nat.pdbx_organelle 
_entity_src_nat.pdbx_cell 
_entity_src_nat.pdbx_plasmid_name 
_entity_src_nat.pdbx_plasmid_details 
_entity_src_nat.details 
1  1 sample 1 104  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
2  1 sample 1 57   ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
3  1 sample 1 55   ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
4  1 sample 1 46   ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
5  1 sample 1 65   ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
6  1 sample 1 38   ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
7  1 sample 1 165  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
8  1 sample 1 121  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
10 1 sample 1 94   ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
11 1 sample 1 2903 ? 'Escherichia coli' 562 ? ? ?   ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
12 1 sample 1 118  ? 'Escherichia coli' 562 ? ? ?   ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
13 1 sample 1 273  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
14 1 sample 1 209  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
15 1 sample 1 201  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
16 1 sample 1 177  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
17 1 sample 1 176  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
18 1 sample 1 50   ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
19 1 sample 1 141  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
20 1 sample 1 142  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
21 1 sample 1 122  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
22 1 sample 1 143  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
23 1 sample 1 136  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
24 1 sample 1 120  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
25 1 sample 1 116  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
26 1 sample 1 114  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
27 1 sample 1 117  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
28 1 sample 1 103  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
29 1 sample 1 110  ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
30 1 sample 1 93   ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
31 1 sample 1 77   ? 'Escherichia coli' 562 ? ? ?   ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
32 1 sample 1 79   ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
33 1 sample 1 77   ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
34 1 sample 1 63   ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
35 1 sample 1 58   ? 'Escherichia coli' 562 ? ? KC6 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
# 
loop_
_chem_comp.id 
_chem_comp.type 
_chem_comp.mon_nstd_flag 
_chem_comp.name 
_chem_comp.pdbx_synonyms 
_chem_comp.formula 
_chem_comp.formula_weight 
A   'RNA linking'       y "ADENOSINE-5'-MONOPHOSPHATE" ? 'C10 H14 N5 O7 P' 347.221 
ALA 'L-peptide linking' y ALANINE                      ? 'C3 H7 N O2'      89.093  
ARG 'L-peptide linking' y ARGININE                     ? 'C6 H15 N4 O2 1'  175.209 
ASN 'L-peptide linking' y ASPARAGINE                   ? 'C4 H8 N2 O3'     132.118 
ASP 'L-peptide linking' y 'ASPARTIC ACID'              ? 'C4 H7 N O4'      133.103 
C   'RNA linking'       y "CYTIDINE-5'-MONOPHOSPHATE"  ? 'C9 H14 N3 O8 P'  323.197 
CYS 'L-peptide linking' y CYSTEINE                     ? 'C3 H7 N O2 S'    121.158 
G   'RNA linking'       y "GUANOSINE-5'-MONOPHOSPHATE" ? 'C10 H14 N5 O8 P' 363.221 
GLN 'L-peptide linking' y GLUTAMINE                    ? 'C5 H10 N2 O3'    146.144 
GLU 'L-peptide linking' y 'GLUTAMIC ACID'              ? 'C5 H9 N O4'      147.129 
GLY 'peptide linking'   y GLYCINE                      ? 'C2 H5 N O2'      75.067  
HIS 'L-peptide linking' y HISTIDINE                    ? 'C6 H10 N3 O2 1'  156.162 
HOH non-polymer         . WATER                        ? 'H2 O'            18.015  
ILE 'L-peptide linking' y ISOLEUCINE                   ? 'C6 H13 N O2'     131.173 
LEU 'L-peptide linking' y LEUCINE                      ? 'C6 H13 N O2'     131.173 
LYS 'L-peptide linking' y LYSINE                       ? 'C6 H15 N2 O2 1'  147.195 
MET 'L-peptide linking' y METHIONINE                   ? 'C5 H11 N O2 S'   149.211 
MG  non-polymer         . 'MAGNESIUM ION'              ? 'Mg 2'            24.305  
PHE 'L-peptide linking' y PHENYLALANINE                ? 'C9 H11 N O2'     165.189 
PRO 'L-peptide linking' y PROLINE                      ? 'C5 H9 N O2'      115.130 
SER 'L-peptide linking' y SERINE                       ? 'C3 H7 N O3'      105.093 
THR 'L-peptide linking' y THREONINE                    ? 'C4 H9 N O3'      119.119 
TRP 'L-peptide linking' y TRYPTOPHAN                   ? 'C11 H12 N2 O2'   204.225 
TYR 'L-peptide linking' y TYROSINE                     ? 'C9 H11 N O3'     181.189 
U   'RNA linking'       y "URIDINE-5'-MONOPHOSPHATE"   ? 'C9 H13 N2 O9 P'  324.181 
VAL 'L-peptide linking' y VALINE                       ? 'C5 H11 N O2'     117.146 
ZN  non-polymer         . 'ZINC ION'                   ? 'Zn 2'            65.409  
# 
_exptl.entry_id         EMD-0322 
_exptl.details          ? 
_exptl.method           'ELECTRON MICROSCOPY' 
_exptl.method_details   ? 
# 
_struct_keywords.entry_id        EMD-0322 
_struct_keywords.text            'Protein folding, ribosomal exit tunnel, nascent chain, titin I27 domain, RIBOSOME' 
_struct_keywords.pdbx_keywords   RIBOSOME 
_struct_keywords.pdbx_details    ? 
# 
loop_
_struct_ref.id 
_struct_ref.db_name 
_struct_ref.db_code 
_struct_ref.entity_id 
1  UNP RL24_ECOLI  1  
2  UNP RL32_ECOLI  2  
3  UNP RL33_ECOLI  3  
4  UNP RL34_ECOLI  4  
5  UNP RL35_ECOLI  5  
6  UNP RL36_ECOLI  6  
7  UNP RL10_ECOLI  7  
8  UNP RL7_ECOLI   8  
9  UNP LPTN_ECOLI  9  
10 UNP RL25_ECOLI  10 
11 GB  CP011113.2  11 
12 GB  CP030939.1  12 
13 UNP RL2_ECOLI   13 
14 UNP RL3_ECOLI   14 
15 UNP RL4_ECOLI   15 
16 UNP RL5_ECOLI   16 
17 UNP RL6_ECOLI   17 
18 UNP RL9_ECOLI   18 
19 UNP RL11_ECOLI  19 
20 UNP RL13_ECOLI  20 
21 UNP RL14_ECOLI  21 
22 UNP RL15_ECOLI  22 
23 UNP RL16_ECOLI  23 
24 UNP RL17_ECOLI  24 
25 UNP RL18_ECOLI  25 
26 UNP RL19_ECOLI  26 
27 UNP RL20_ECOLI  27 
28 UNP RL21_ECOLI  28 
29 UNP RL22_ECOLI  29 
30 UNP RL23_ECOLI  30 
31 GB  CP032204.1  31 
32 UNP RL27_ECOLI  32 
33 UNP RL28_ECOLI  33 
34 UNP RL29_ECOLI  34 
35 UNP RL30_ECOLI  35 
36 UNP TITIN_HUMAN 36 
# 
_em_3d_fitting.entry_id          EMD-0322 
_em_3d_fitting.id                1 
_em_3d_fitting.details           ? 
_em_3d_fitting.overall_b_value   ? 
_em_3d_fitting.ref_protocol      ? 
_em_3d_fitting.ref_space         ? 
_em_3d_fitting.target_criteria   ? 
_em_3d_fitting.method            ? 
# 
_em_3d_reconstruction.entry_id                    EMD-0322 
_em_3d_reconstruction.id                          1 
_em_3d_reconstruction.algorithm                   ? 
_em_3d_reconstruction.details                     ? 
_em_3d_reconstruction.fsc_type                    ? 
_em_3d_reconstruction.refinement_type             ? 
_em_3d_reconstruction.image_processing_id         1 
_em_3d_reconstruction.num_class_averages          ? 
_em_3d_reconstruction.num_particles               301510 
_em_3d_reconstruction.resolution                  3.2 
_em_3d_reconstruction.resolution_method           'FSC 0.143 CUT-OFF' 
_em_3d_reconstruction.symmetry_type               POINT 
_em_3d_reconstruction.method                      ? 
_em_3d_reconstruction.nominal_pixel_size          ? 
_em_3d_reconstruction.actual_pixel_size           ? 
_em_3d_reconstruction.magnification_calibration   ? 
# 
_em_buffer.id            1 
_em_buffer.details       ? 
_em_buffer.pH            7.5 
_em_buffer.specimen_id   1 
_em_buffer.name          ? 
# 
loop_
_em_entity_assembly.id 
_em_entity_assembly.parent_id 
_em_entity_assembly.details 
_em_entity_assembly.go_id 
_em_entity_assembly.name 
_em_entity_assembly.source 
_em_entity_assembly.type 
_em_entity_assembly.entity_id_list 
_em_entity_assembly.ipr_id 
_em_entity_assembly.synonym 
_em_entity_assembly.number_of_copies 
_em_entity_assembly.oligomeric_details 
1 0 ? ? 'TnaC-stalled ribosome complex with the titin I27 domain folding close to the ribosomal exit tunnel' 'MULTIPLE SOURCES' 
RIBOSOME 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36 ? ? ? ? 
2 1 ? ? ribosome                                                                                             NATURAL            
RIBOSOME 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36 ? ? ? ? 
3 1 ? ? 'Tryptophanase operon leader peptide'                                                                RECOMBINANT        
COMPLEX  9                                                                                                  ? ? ? ? 
4 1 ? ? 'Titin nascent chain'                                                                                RECOMBINANT        
COMPLEX  36                                                                                                 ? ? ? ? 
# 
_em_image_scans.entry_id                EMD-0322 
_em_image_scans.id                      1 
_em_image_scans.dimension_height        ? 
_em_image_scans.dimension_width         ? 
_em_image_scans.frames_per_image        30 
_em_image_scans.image_recording_id      1 
_em_image_scans.sampling_size           ? 
_em_image_scans.scanner_model           ? 
_em_image_scans.used_frames_per_image   ? 
_em_image_scans.citation_id             ? 
_em_image_scans.number_digital_images   ? 
_em_image_scans.od_range                ? 
_em_image_scans.quant_bit_size          ? 
_em_image_scans.details                 ? 
# 
_em_imaging.id                              1 
_em_imaging.entry_id                        EMD-0322 
_em_imaging.accelerating_voltage            300 
_em_imaging.alignment_procedure             ? 
_em_imaging.c2_aperture_diameter            70 
_em_imaging.calibrated_defocus_max          ? 
_em_imaging.calibrated_defocus_min          ? 
_em_imaging.calibrated_magnification        ? 
_em_imaging.cryogen                         NITROGEN 
_em_imaging.details                         ? 
_em_imaging.electron_source                 'FIELD EMISSION GUN' 
_em_imaging.illumination_mode               'FLOOD BEAM' 
_em_imaging.microscope_model                'FEI TITAN KRIOS' 
_em_imaging.mode                            'BRIGHT FIELD' 
_em_imaging.nominal_cs                      2.7 
_em_imaging.nominal_defocus_max             3000 
_em_imaging.nominal_defocus_min             1000 
_em_imaging.nominal_magnification           ? 
_em_imaging.recording_temperature_maximum   ? 
_em_imaging.recording_temperature_minimum   ? 
_em_imaging.residual_tilt                   ? 
_em_imaging.specimen_holder_model           'FEI TITAN KRIOS AUTOGRID HOLDER' 
_em_imaging.specimen_id                     1 
_em_imaging.sample_support_id               1 
_em_imaging.citation_id                     ? 
_em_imaging.detector_id                     1 
_em_imaging.scans_id                        1 
_em_imaging.date                            ? 
_em_imaging.temperature                     ? 
_em_imaging.tilt_angle_min                  ? 
_em_imaging.tilt_angle_max                  ? 
_em_imaging.astigmatism                     ? 
_em_imaging.detector_distance               ? 
_em_imaging.electron_beam_tilt_params       ? 
_em_imaging.specimen_holder_type            ? 
# 
_em_sample_support.id               1 
_em_sample_support.specimen_id      1 
_em_sample_support.details          ? 
_em_sample_support.grid_material    COPPER 
_em_sample_support.grid_mesh_size   ? 
_em_sample_support.grid_type        'Quantifoil R2/2' 
_em_sample_support.method           ? 
_em_sample_support.film_material    ? 
# 
_em_vitrification.id                      1 
_em_vitrification.specimen_id             1 
_em_vitrification.chamber_temperature     277.15 
_em_vitrification.cryogen_name            ETHANE 
_em_vitrification.details                 ? 
_em_vitrification.humidity                100 
_em_vitrification.instrument              'FEI VITROBOT MARK IV' 
_em_vitrification.entry_id                EMD-0322 
_em_vitrification.sample_preparation_id   ? 
_em_vitrification.citation_id             ? 
_em_vitrification.method                  ? 
_em_vitrification.temp                    ? 
_em_vitrification.time_resolved_state     ? 
# 
_em_experiment.entry_id                EMD-0322 
_em_experiment.id                      1 
_em_experiment.aggregation_state       PARTICLE 
_em_experiment.reconstruction_method   'SINGLE PARTICLE' 
_em_experiment.entity_assembly_id      1 
# 
_em_admin.entry_id              EMD-0322 
_em_admin.current_status        REL 
_em_admin.deposition_date       2018-10-26 
_em_admin.deposition_site       PDBE 
_em_admin.details               ? 
_em_admin.last_update           2024-10-23 
_em_admin.map_release_date      2018-12-05 
_em_admin.obsoleted_date        ? 
_em_admin.title                 
'TnaC-stalled ribosome complex with the titin I27 domain folding close to the ribosomal exit tunnel' 
_em_admin.header_release_date   2018-12-05 
# 
loop_
_em_author_list.ordinal 
_em_author_list.author 
1 'Su, T.'    
2 'Kudva, R.' 
# 
_em_ctf_correction.id                                1 
_em_ctf_correction.em_image_processing_id            1 
_em_ctf_correction.type                              'PHASE FLIPPING AND AMPLITUDE CORRECTION' 
_em_ctf_correction.details                           ? 
_em_ctf_correction.amplitude_correction              ? 
_em_ctf_correction.amplitude_correction_factor       ? 
_em_ctf_correction.amplitude_correction_space        ? 
_em_ctf_correction.correction_operation              ? 
_em_ctf_correction.phase_reversal                    ? 
_em_ctf_correction.phase_reversal_anisotropic        ? 
_em_ctf_correction.phase_reversal_correction_space   ? 
# 
_em_db_reference.id             1 
_em_db_reference.access_code    6I0Y 
_em_db_reference.db_name        PDB 
_em_db_reference.details        ? 
_em_db_reference.relationship   'IN FRAME' 
# 
loop_
_em_entity_assembly_naturalsource.id 
_em_entity_assembly_naturalsource.entity_assembly_id 
_em_entity_assembly_naturalsource.cell 
_em_entity_assembly_naturalsource.cellular_location 
_em_entity_assembly_naturalsource.ncbi_tax_id 
_em_entity_assembly_naturalsource.organ 
_em_entity_assembly_naturalsource.organelle 
_em_entity_assembly_naturalsource.organism 
_em_entity_assembly_naturalsource.strain 
_em_entity_assembly_naturalsource.tissue 
2 2 ? ? 562  ? ? 'Escherichia coli' KC6 ? 
3 3 ? ? 562  ? ? 'Escherichia coli' KC6 ? 
4 4 ? ? 9606 ? ? 'Homo sapiens'     ?   ? 
# 
loop_
_em_entity_assembly_recombinant.id 
_em_entity_assembly_recombinant.entity_assembly_id 
_em_entity_assembly_recombinant.cell 
_em_entity_assembly_recombinant.ncbi_tax_id 
_em_entity_assembly_recombinant.organism 
_em_entity_assembly_recombinant.plasmid 
_em_entity_assembly_recombinant.strain 
1 3 ? 562 'Escherichia coli' ? KC6 
2 4 ? 562 'Escherichia coli' ? KC6 
# 
loop_
_em_euler_angle_assignment.id 
_em_euler_angle_assignment.details 
_em_euler_angle_assignment.image_processing_id 
_em_euler_angle_assignment.order 
_em_euler_angle_assignment.proj_matching_angular_sampling 
_em_euler_angle_assignment.proj_matching_merit_function 
_em_euler_angle_assignment.proj_matching_num_projections 
_em_euler_angle_assignment.type 
1 ? 1 INITIAL ? ? ? 'PROJECTION MATCHING' 
2 ? 1 FINAL   ? ? ? 'PROJECTION MATCHING' 
# 
_em_final_classification.id                         1 
_em_final_classification.type                       ? 
_em_final_classification.avg_num_images_per_class   ? 
_em_final_classification.details                    ? 
_em_final_classification.image_processing_id        1 
_em_final_classification.num_classes                ? 
# 
_em_grid_pretreatment.id                  1 
_em_grid_pretreatment.sample_support_id   1 
_em_grid_pretreatment.atmosphere          ? 
_em_grid_pretreatment.pressure            ? 
_em_grid_pretreatment.time                ? 
_em_grid_pretreatment.type                ? 
# 
_em_image_processing.id                   1 
_em_image_processing.image_recording_id   1 
_em_image_processing.details              ? 
# 
_em_image_recording.id                            1 
_em_image_recording.imaging_id                    1 
_em_image_recording.avg_electron_dose_per_image   0.926 
_em_image_recording.average_exposure_time         ? 
_em_image_recording.details                       ? 
_em_image_recording.detector_mode                 ? 
_em_image_recording.film_or_detector_model        'GATAN K2 QUANTUM (4k x 4k)' 
_em_image_recording.num_diffraction_images        ? 
_em_image_recording.num_grids_imaged              1 
_em_image_recording.num_real_images               2613 
# 
_em_imaging_optics.id                         1 
_em_imaging_optics.imaging_id                 1 
_em_imaging_optics.chr_aberration_corrector   ? 
_em_imaging_optics.energyfilter_lower         ? 
_em_imaging_optics.energyfilter_name          ? 
_em_imaging_optics.energyfilter_upper         ? 
_em_imaging_optics.energyfilter_slit_width    ? 
_em_imaging_optics.phase_plate                ? 
_em_imaging_optics.sph_aberration_corrector   ? 
# 
_em_map.entry_id               EMD-0322 
_em_map.id                     1 
_em_map.annotation_details     'Titin I27 domain folded at the exit of ribosomal tunnel' 
_em_map.axis_order_fast        X 
_em_map.axis_order_medium      Y 
_em_map.axis_order_slow        Z 
_em_map.cell_a                 394.31998 
_em_map.cell_alpha             90.0 
_em_map.cell_b                 394.31998 
_em_map.cell_beta              90.0 
_em_map.cell_c                 394.31998 
_em_map.cell_gamma             90.0 
_em_map.contour_level          0.019 
_em_map.contour_level_source   author 
_em_map.data_type              'Image stored as floating point number (4 bytes)' 
_em_map.dimensions_col         372 
_em_map.dimensions_row         372 
_em_map.dimensions_sec         372 
_em_map.endian_type            big 
_em_map.file                   emd_0322.map.gz 
_em_map.format                 CCP4 
_em_map.label                  ::::EMDATABANK.org::::EMD-0322:::: 
_em_map.limit_col              371 
_em_map.limit_row              371 
_em_map.limit_sec              371 
_em_map.origin_col             0 
_em_map.origin_row             0 
_em_map.origin_sec             0 
_em_map.partition              1 
_em_map.pixel_spacing_x        1.06 
_em_map.pixel_spacing_y        1.06 
_em_map.pixel_spacing_z        1.06 
_em_map.size_kb                205916416 
_em_map.spacing_x              372 
_em_map.spacing_y              372 
_em_map.spacing_z              372 
_em_map.statistics_average     1.5069403E-4 
_em_map.statistics_maximum     0.2401755 
_em_map.statistics_minimum     -0.17072792 
_em_map.statistics_std         0.011110076 
_em_map.symmetry_space_group   1 
_em_map.type                   'primary map' 
# 
_em_particle_selection.id                       1 
_em_particle_selection.image_processing_id      1 
_em_particle_selection.details                  ? 
_em_particle_selection.method                   ? 
_em_particle_selection.num_particles_selected   468015 
_em_particle_selection.reference_model          ? 
# 
loop_
_em_software.id 
_em_software.category 
_em_software.details 
_em_software.name 
_em_software.version 
_em_software.image_processing_id 
_em_software.fitting_id 
_em_software.imaging_id 
1  'SYMMETRY DETERMINATION'   ? ?      ?   1 1 1 
2  'IMAGE ACQUISITION'        ? ?      ?   ? ? 1 
3  MASKING                    ? ?      ?   ? ? ? 
4  'CTF CORRECTION'           ? Gctf   ?   1 ? ? 
5  'LAYERLINE INDEXING'       ? ?      ?   ? ? ? 
6  'DIFFRACTION INDEXING'     ? ?      ?   ? ? ? 
7  'MODEL FITTING'            ? ?      ?   ? ? ? 
8  'MODEL REFINEMENT'         ? ?      ?   ? ? ? 
9  OTHER                      ? ?      ?   ? ? ? 
10 'INITIAL EULER ASSIGNMENT' ? ?      ?   1 ? ? 
11 'FINAL EULER ASSIGNMENT'   ? ?      ?   1 ? ? 
12 CLASSIFICATION             ? ?      ?   1 ? ? 
13 RECONSTRUCTION             ? RELION 2.1 1 ? ? 
# 
_em_specimen.id                      1 
_em_specimen.experiment_id           1 
_em_specimen.concentration           ? 
_em_specimen.details                 ? 
_em_specimen.embedding_applied       NO 
_em_specimen.shadowing_applied       NO 
_em_specimen.staining_applied        NO 
_em_specimen.vitrification_applied   YES 
# 
_em_start_model.id                               1 
_em_start_model.details                          ? 
_em_start_model.image_processing_id              1 
_em_start_model.emdb_id                          ? 
_em_start_model.insilico_model                   ? 
_em_start_model.orthogonal_tilt_angle1           ? 
_em_start_model.orthogonal_tilt_angle2           ? 
_em_start_model.orthogonal_tilt_num_images       ? 
_em_start_model.other                            ? 
_em_start_model.pdb_id                           4UY8 
_em_start_model.random_conical_tilt_angle        ? 
_em_start_model.random_conical_tilt_num_images   ? 
_em_start_model.type                             'PDB ENTRY' 
# 
_em_support_film.id                  1 
_em_support_film.sample_support_id   1 
_em_support_film.material            ? 
_em_support_film.thickness           ? 
_em_support_film.topology            ? 
# 
_em_tomography_specimen.id                       1 
_em_tomography_specimen.specimen_id              1 
_em_tomography_specimen.cryo_protectant          ? 
_em_tomography_specimen.details                  ? 
_em_tomography_specimen.fiducial_markers         ? 
_em_tomography_specimen.high_pressure_freezing   ? 
_em_tomography_specimen.sectioning               ? 
# 
_pdbx_audit_support.funding_organization   'Knut and Alice Wallenberg Foundation' 
_pdbx_audit_support.country                Sweden 
_pdbx_audit_support.grant_number           ? 
_pdbx_audit_support.details                ? 
_pdbx_audit_support.ordinal                1 
# 
