2018-11-21 REL PDBe PDBe PDBe 2018-09-20 2018-10-24 2018-11-21 2018-11-21 Structure of K. phaffii TFIID Kolesnikova O Ben-Shem A Luo J Ranish J Schultz P Papai G Kolesnikova O Ben-Shem A Luo J Ranish J Schultz P Papai G Molecular structure of promoter-bound yeast TFIID. Nat Commun UK 9 4666 4666 2018 30405110 doi:10.1038/s41467-018-07096-y 2041-1723 EMD-0249 associated EM volume Transcription Factor IID Transcription Factor IID 0 Komagataella phaffii GS115 0.9 singleParticle particle 0.4 8. Quantifoil R2/2 COPPER/RHODIUM 300 GLOW DISCHARGE AIR ETHANE 95 283 FEI VITROBOT MARK IV
Monodisperse
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 0.01 1.5 0.85 3.8 4.322 50000. FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN Cs corrector is present on the microscope FEI FALCON II (4k x 4k) INTEGRATING 4096 4096 14.0 1-7 1.0 50.0 1 255723 Gctf
single particle cryo-tomography
 C1 FOURIER SPACE 12.13 FSC 0.143 CUT-OFF RELION 155620 MAXIMUM LIKELIHOOD RELION MAXIMUM LIKELIHOOD RELION 3 RELION emd_0249.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 160 160 160 0 0 0 160 160 160 352.0 352.0 352.0 90.0 90.0 90.0 X Y Z -0.06075021 0.058848996 -0.00024534267 0.0064801024 2.2 2.2 2.2 0.028 AUTHOR komagataella phaffii (formerly Pichia pastoris) TFIID