2024-10-16 REL PDBe PDBe PDBe 2018-08-31 2018-09-26 2018-09-26 2024-10-16 Swiss National Science Foundation Switzerland Ramrath D Niemann M mitoribosome, translation, Trypanosoma, small ribosomal subunit, 9S rRNA, ribosomal protein, mitochondrial initiation factor IF-3, RIBOSOME Ramrath DJF Niemann M Leibundgut M Bieri P Prange C Horn EK Leitner A Boehringer D Schneider A Ban N Science US 362 2018 30213880 doi:10.1126/science.aau7735 1095-9203 0038 SCIEAS 6hiw FULLOVERLAP small subunit of the T. brucei mitoribosome in complex with mt-IF-3 small subunit of the T. brucei mitoribosome in complex with mt-IF-3 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 Trypanosoma brucei brucei Lister 427 2.4 mS48 Trypanosoma brucei brucei 0.20181118799999997 1 LEVO MMRRVASTGSNGGHCYVGSSLWADSVYMEVSSITLQKRFSFKYATKLQHDEMRQPYYIHEKRYGIFSNERNIAKARRGLP FITPLYTKHMNLWDTDTDASNNRFFRGYYYGQRELHQLLGRPHSLEATNADGSNDLSTYEANTSQLYKGIPRPAITNLHY EPAWRYTLYQAGAHGAQLSNPRSPFTAKVLGDELMQVRDIKSVEHCKAWFDRLQYLINLHYEAVGDIGEFKSRHTRHVHE FFVAFHDALSSLDFRDTYLFDQFKAVRPPELSDLFGIFLEMEANYVHEDYCPRCSLPYSTTRYCGEGDVDTPFRKHRGRW APHQKWGREWYDVVARRAEALWYRATEDPYFGTPQHTQRQAEALLKVYVQAKQRGKAIDFMNKLRGSVEYLTGDITITTV MQESYDALLDTTPHPHLLTNGFTLESDAAKYTGEVNKAPLSPLQFRIDMEMNKYRRQQKEEGVVRVPPALWRLDTSAIVP YKVDSKTRRIVNWREVKEGIEKSFLSVGLPKEAYTGNEWREMLYLHDVIANREAKAAVLEQQQKLDREKLKARERASLKG EKSSNSASNGIGIIFPDKDGYQVFFVDEASLRPFGIGASGTLFKAVARVYPSPAAVPYDDPVHGKQFLIDTTDETCHLFG GFEHGDRLLLRAKSSGGDVDGGNNSNCRNTGSFTDEEEEVIVMGVSTEGSDAERMLCAMHVDTGKQRERGIVFLGTDCID IRERWESVRYAASAYVKGRVTLLESQRTAREEFMGQVVGIRDGVLFVQWRLLKGGGSVLDRSVAVPIGDSTQVRELFVET KVLGVEPLQSPPSWRTPFRNDFAEERLKELQRAPFKREKYVSLIQGKYTPKVKKFGYTQHTTVDDFETKEYKDRLLSKQF FQNPQAFEVVPDRHERSVQFGGKWEYQRTHGLPTVDRNELENGWSEVEPISDGEMKVIEQALRDISGPRPGNFVKPPSKT KSLQLSESWWEPLGYGWEQHNEEQKALCDVTEQRLIDGSNLPFGGKLPPFGTSYGMGERMRAIIEDYSKGFGLGPHGHSP THDTIHYNTLNAEGERVRDLGYTDALGRLFSEKLGDRDVHQWAVESCADGEADVRQLLLSLHEWRERGRPPSLLLANVLS KYLEEEIAAFNKGIPENAPKLQLETSDGTLAHSGSGGSRSGTMWADVDPTTFALHQASQSTRSTRCDEPFILHLVKRAKL GECVTNFTDTAYIAHLESSVINEFHLALTKLVGKGISPTLLAQKTGQLHRGSVRVGGNVVPFVKSRELSRLLERMGLTSE SIAVITRGLANCPEQESVGDDFAVPVSLILSWDGPGSSGGSGSGDRRLNTTAARNEQQRKGSAALSSAIRQLSQHQKQRG SASGRGWQNEDKMVVHVLEELSLRNDGLVMDIQYAIRENKKNRRLRWEFVTTLLPVFGGNEAKVEQLYSDYCDGKYVPNI TVAVEAFIAFLHNATQHPETYSASDYFDIDDGKSSTASGTGDQPSAGQYTTLKLLDPLEGPFVFDNVKVEYIQTVERFRR HGIRAGPVMAPATGFIAANCKSLNYFTRRQEEVVYVTNDSDQGLRRSLENSAYHKTIAANPALQYLLKARRGAALVETFN RFFYRTMPMLNFYQNVLKHYSETIQPLRQAAKSSTRGLARALESERSAAMEEFRRNSERYWRNIIEGRSVEQVVLANGES NRRTVGGGAGEQLQKPQGNRDAVRTNFTGEATDGSRKGQRQHQQPPLSQQQQHSRGEGERSAVHTSPRQGGSRGMADLLS KLNTPKTGSGVKGSTAKNTGNNRRGPKS Q57UJ2 mS51 Trypanosoma brucei brucei 0.09508099199999999 1 LEVO MLRCTVVGHHRSGKARAFVFRDPSLRMMRAGSGYQQLRRMGMPMQVGMGWRKVDSFHANTQYQHAWPLLSHDDLGNSDQS NNTKNIMYSMYMPKRNKGTAPWFRGADTYSVKYCEQGRYEYQRYLMINRFPSEYKKHFLSFLSNIRMSSGSATIPQEALH WLLRMIVDNFNPQHVHYIAAMKTLQSAGELDMARDVWKIMERQQTWPCTATICAYLDVCVEAGEKTWAMEAWNRYCTELK FLEPGEVDPKPISRVPFSLTREELLYLPKWKKHFDHDPNLDVMDLNRFNRTREVYLRMAQVMLAGGERNAFQHFFTKLEE AMLNKPTPVPEPPNPHLVRRPRWAPYEHCKSVHHSPWRLQNNGRALALGPPVTIEDEMQSRFFSNDQFLVHSVKEVLRIV LQEHKRAHPTECTRCKTEAFFYKTKDADETLKFCDDLIERLFASLGVRLSNLNTSSLLSTILEVFRVVGKESGAALLQRA NEFLERKASLGDAEGSRENLTASNYLQVLSGFADESAFVYNTKKDGTCQYKTGFDPRTTMRHLADVVQEIAGNPHVTWAA DMHLQVVETMVGCGTMKANDYFVRNVLRQFSWDSRFLEALYVEYRRQDDVDMWAELTKRALVWTARYNAPASERLRRLIE DDYDTIRVQTRTFRELAVFQFRDVEERRHSRDVVNELPNPWYDYVAHALPFPDRDAGYPDEYGDLGQWRAPGGPGSPVRG PGYYAPPMEGEHQRGYTAEWRDLRNPMRPPEFPTPWERKYRQYARGQHPSYDMVYAGPMPEIFPMRRDFRKPTRWDFHDI EKQGKYRTSGPY Q385L8 mS56 Trypanosoma brucei brucei 0.047306836 1 LEVO MRKFCHFMANCWNSARSHATYGAVPLTHSQVTSVYATDGGKVDELGLLELVEERIFSWKLNKWEMRIPPNLPNDQKELIR QEQENLKQILSEWRKCFGALNADILQISSLTGVPKDVVREKNRTWLQEEVAKLRWMGEVNKAALLRDAFMRLEAFGSRDF MFMERLCCIYGLARQGTFDEAFTNYITEDPVTNDIFVDERNPFKELVAHIVRNYSQIDIIYDFLGFNYSEGYRSSLRRYM EYLQCKTAENVRASGRLVTGDKGEHNILFDYCVSRESLVSGDSCQGIIDFLYINGNDVTLIIIASDNPWLRNRQLPHRRQ MEGIARRVCFVLGIPPSEVRIRNLLLPPTYLDKGSIVRLNDIVFRLSNEQSNLLIPWLTNYNKELDPKDVDYTALAKTTN EEEWLTL Q587C2 mS59 Trypanosoma brucei brucei 0.035395676 1 LEVO MRCSCAFLDKSVFAAKRRVIVPIHPTPNFPAHFIKSAFTTDPLKEKQKARFSSGGEAMREVQDIPKNLEGERSRRDLASR GDTEFQALVEFIEGASYDQLISGRRFKKVYDVLSENDDMFIWLCHTAMSVLNPGDMRSRLIYNHLRILAESVASGEMTQR TAFRFFESAVRSPAYREIAKRQLEGGAATRLAGISAAADVMRRMGLTRRPMSSYFELYQRIVERSEAMTPWGFPPLFQFE ERLSLEPRLKFFSRASQQSLERRRRGHVMTPHTTLHGRRIFWIPPTWNRAGRFLGPHVTLYPGMTPD Q38BS2 mS60 Trypanosoma brucei brucei 0.034546641 1 LEVO MEVYRRITERVRGALWAHHGRLMSERQFHRILAFVYNNKYEYQIRFDRMEVVGREWGGRVEVVTTPAGFLKRVRVNPCVE DLTLYQQQQLILSAYANACADGRKLMEQAEMNIYKQFLKDLKPIVMGIRDNPEFYTVSENSIETIGGVLQTGDSNNLCHT HRTIPAAKARQPADEVRARQEWEQKWINSPRGQSWALTLRGKTYFALHGPQYRPRGAPGAKKETLPLDIPAPYTAMDEKR LLKKNWMAYLDNKHVAEVMWTRVKIADREKRQRLLQETGRAWHRPINEEAINRW Q57XL2 mS61 Trypanosoma brucei brucei 0.03348233999999999 1 LEVO MLCRTFLRQFRMSGGDMFVEYKVLSRDHRRSIRVEDAIVDPTFKRTVLPLGWLELLRSPSLRLPTGYFVEETVHVSLPNA TSNGGKKEARPQKGGFASGSPSVGRNEANAIIAGPVVLYITGQSVPVVLNPYFVPEGTWDMRTRDGELDLRLGMDAIEQC TLFSELRPGGLLYGKLPENPNVRRNESLRATLGRYGMKCDLAESPLVPRPWTRMRYMFIDELQRGPKLTEFVGHNPRNGT PWRFSQNTKYFRIGIWRDTIRRNDMNEGLHAHSSWQKSPQQSVPEVRFLAPYP Q38D60 mS62 Trypanosoma brucei brucei 0.032068099999999995 1 LEVO MRRFCTARVSSYVKRAPLLLPRGGRRWRSGAGTTADGGGEKEYIADSFESTAGSNAYAAMELAAEARREIHELWLSAETA LEREKRVQQVAALIEKYKLDPSTPREADVSRGLGDAFDRLLLLCLPLGKTDAKGTDNLERLMHLAGRNGRELSVRTIQHL FARTDSFAEALAVFYTMRRCHVAMNMEAYYSMLYSLQRLEEEGWGQHFRNEYEENGAPSEQAMDFIVKGISNALLPENKP WLGRVMFQDRNVPDRRYDTRDFDELDTAWTQRYKSGTPAGAH Q383D1 mS63 Trypanosoma brucei brucei 0.031922177999999996 1 LEVO MLHGTPVRRASLRYRRPYWMMFLKGVDNWKIYTVIQQPDHQRTEMLYQAWLGGLDRPYTRPKCMANQPLWLSKKRHMLRK ERLDGPETPLEKYVLEWHKKFHSFQGTERPTPDDLHTALDLVERPLDLSYALQLLGQCRNLNNIRFAKETFLVFLEACLR VGRRDCAEYALEHAEPLGFWFIDEDHRRYLQGEQTWYKLSPLDNLYYPVEENAKLNEGRKPITRLSPATESPGSGTAISD GEPSTDVEGETTVDDEIAQLEAELAALEREGGGK Q38F25 mS64 Trypanosoma brucei brucei 0.030742206999999997 1 LEVO MLRRVPRVLQKGERGLYFPINHRIVDHRIAPGVTVEETDVQSRYRRELRTNFATGETRQTIAPPWSARERPTHFFSLRLP SRNSVTLRVKEMHNHLLFSHQQCAPMLVSPTKLHITLGVMTIPENSREELVKNIQQSMSETFACVQPLQLRFRGLGTFDF GRVLFIRVVPEADFGRLERAVCDIRRRVGGVLGVDLKGNPHDSYVPHVTVAKIRPNQRQQLGDTIPQSLWADYQHHDFGD VTLSTVDLCRMKGSPDGYYHTEGAVHLS Q382E8 mS65 Trypanosoma brucei brucei 0.030617381 1 LEVO MFSESLCILSRRFRYNTKFPALVSYNKLPWEVVNHETPQFHMHVAPHYEQLLTLAASSPVPHIIGSKHIDVPREHRLRLL PGMLYLLDGDTLPGEFTINRVLDPTALQYYGRLSSQIVTVEAVRMLVPDDLRLLCNCITFKGPLHLPVAPYASLASLRGA SQGGTTGSETGSNCFTLYHFVRPNRPPKELQLEKYYIHAPCVAPLSEFASNSDERGNWRPRLQAPKRTRRATPLPAYRPP QSYLMGLAERLAVVPGGCFGRRSLMWGHWF Q57UA2 mS66 Trypanosoma brucei brucei 0.029960182 1 LEVO MFNRNFILRAAEFKQKSRWSSVWPNMRYGSMFLSYSVGRKLPMKGVNWVTRDSNRLLNFSNRYSAVISDIDVKRTEEELN VALSDIRWNDHRRIYWKCSFCGSSYRKNVSVRTKYHAGCNFCKKKYPSEVRGEQHGSPPVSQAAPELTEQLVDNGKRDNL ATLAVTSKFYAEWTCRGCGGSYRSTIRSRTGQVEAGQCTLHPDIVAWSAYCPSCSWRPNMVPIAEEVSRSGQFLGLERSM EELGGQRGKQLPIPRRKRLAC Q388L7 mS68 Trypanosoma brucei brucei 0.026472807 1 LEVO MRRGALSLLKAGLLGHYQQEAFEARKRFEESTTYPGPIRAATPGDTRFYSGSLESILHDTDRHYWRAVTDDPRVQHLIPL RIRFKIFTWVTSGWEQRMQVVQIMAPKDSTIAQVKDLVIVENQSPYLCVSSFHLAIDGKELDPQKTLGEYGITEQSQIDA IEQNDHLLHRDDERPRDWTVDEITAEDVKRSPYKEMEMQPLQNLAPRYEARPKGYFGRTYYSGMKQSS Q582T9 mS73 Trypanosoma brucei brucei 0.011360885 1 LEVO MLRRSILFRMKYADLELTTRGEFPHGMKEPAFVKKLDQNIPWYFSTYRSMYHWPITGDNWSDLNEAEKHHDLHMFYTLAW WKLGEGIFGVDEDS Q587C4 mS74 Trypanosoma brucei brucei 0.008203407999999999 1 LEVO MFSLTQTWLIAHWYCGHKFRHRFMRDKRFHPSLQASHDARNRFSKRRHFKTNRWNYQQAYRDMP mS49 Trypanosoma brucei brucei 0.13723412499999998 1 LEVO MIRRRVCDGARLAPNIRATFSAVRYQSGLHTFIRDSKPSNFSSVRRSENANGDATASPGATAGENPASSGDWASHMQREL FGEVDPLGGQAHKDYYRDVTRGYSPQYAPRNFANGGAVAYPHIQSPYEYEEAAHRRVWLDHDVDRMREEFTQHRASLRSL ASAQEREELLRSRAAEYQVANTVHESESVHPIQQLYNSGGTSRSALKQQAVADRYSIAEQHSPLPLTTGVDRDALDEAQR TKDRILNDSFTAENLLITHGLREKEKHDFTILQRTVRIPFQGYDMDRFLAQQKGTPYGAQQLPPNVVPSSMEEAQRTLRG SSATATPLVDAVAQKVYARNTVVDRPAIGEQLTEQIINIMRASRTTAEQQREEERAQRFGLGRQGALVQDGGPDQRTLKK HTNDERIVDAMLFQQNAYRKTPTDEHWNPYIRRSTENGVGHLLQNKFDIMRREDRLSKGEQDLTERNTIHYGVPIQQIVD EFVFRHRNARGERPLDYFKPFPNFRALRLNRMYRDVEGFSLMKQRPEFLEWELFTRYRQHHQQRRRLALLHGLEPVANET AQERDTRRHRLDEICERTPFDEREMRVNDDEMRVSVETLRSWFGVYMLPSPTVVNAVLGGSASVNLHLYHLADEMGTADT REHVLSSRYLNRLLLLESYQNRVGRGFMNHVVGRAPEPVVPHEQPQEVLRHFSAEERAMYEQHVKEQTSRQLGEWERAMK RRRWLTDHQQYGHVVSHGLETSVVDLSHTETGAVLTVSTKAYEQEIEAVRMKTNATIKVDGMVYNLLPNSERRVVPLTVQ LDSGEKIDMTSEDFDRCELEAFPRNLNHALNYGIANYAYNRGNYVETQDSIWEEQTASGQEGWSPATHADGLREGLPVRA RRPIFSSSAEQRIAGGPQRAVIIQYHHQPFFNPEPRLVKVAFQCDGTIMEVPISDVMIWQRRYHGPERTVGDESRRYNPA AMRRYVDVTDPFNEKTSNTEHFLDKYEPKRNADTVADKYRTTKQITEIDKWTRYDSARADNYRPLSISHRRDYIRMGYIP RYTPWEWIAIQEADQPLIAEQIRQDNIGTSYFFSLNRYWRYKASPHGYIRHFENEVRDLLQYVDGVTPWKQAQKIRTYWE VRSHHPMPQFNRPEVAMHRNTVGLLPAHMWETDKKTGKVKSVKDSVRDYQTKTPYPKWVQL Q586P5 mS50 Trypanosoma brucei brucei 0.13171924999999998 1 LEVO MFRVRRLAGFIQCSPCGKMGPLKQQTRRYTPIWKSDPAVDNVAPLRDEDERRALWAEVGPISDVGSAVTAWIRFGNDPVL HTAVPTMLGGKFRNQQREKESLLPNSSSPFAYVEDYMGTNLVFGSPVHAKESAAVWATYFERRYASRLRLSRRTVANYVG LINSPEVFDDESDRPETRWSQDTFFRECAYLSEKFLKEKVSNMQQFEAALKRASPEAYLAFFDAFQQQTQTQIPLPSPSV WHYEGERRKQWAEKFISISHKAQAFFKDVLSEDVKKYQEVPGKLLQKVKPVLADVGKILVKRHERWLKGRVWTSLTEEER EAYCMKEVKRQQMQVEDGEFDPMMEDDVDDTELEEWQREHDAIMKLMNSPIDGLHFTTLELWLHTMRCEELETEHIYTSA RVRAIQVAARKKLYDTTSYEEVIQAVVESIARGTLDLGAGVLRPHFNEVWCQLNYAKFGSSTITQHTTTSRRQLLFFHAG SLKDIAATATLYYATKPLSNSLDYASPYKYRRSLITLCSNYGVETAYTTQRPLLRSAANLARAEDLIHAVVTAAAQPFGE RRRAATRDLHMEFQRLAVPVERVIVANPVSALLESGADPDEKPVEGEKVNMWPLGAKRVVLYKWSAPNVEKLKAMESDAA SAVSGSSLTAKRLREIQELKRRGFLEVSLWRRVTAQERKQRNEIVEAKKKQVEEVVRTVPSLAHLHQYATSLYSRIEERV AFPTETSTVTETTNMKEETNKPLEDSEWEFAVLLDDRVLLNKEESVELYLPYRDANGELLAQGEYRALVRAFDLEANPNL HPAYCSVGYSESFQVFDALPQLIAQFFRVKDATAEAAGVTHIPAADFTPFCAFLRDAGLDVPLRCEFEAGQAVTTDGDVY MDYFLQLLRGEAFHQSHAQAGLTEAQRAIEPLCRAHWVVHHPGADESEWATARRSVLDHAMQHEREWWFPNEMLDVKDVV TGSTNGLTPQMYPAAVRYGVELCTVLTAEGKFVDERGSGLSARCVVNGTGAAESVVFDTANCNGTNTTSVEDALRVAHGA LRSAQDRHNTLAAFRLGPLSKQSQVLLFCGVNAYEFGGKYARTYAYAFEKAKKELEATAASGFMAPSLSHEDTERLSDQP TTSPSVDRFASTTHPEQRKAQFVPRVGPGSTPLEDPAADQKSEWS Q57YB5 mS52 Trypanosoma brucei brucei 0.085012258 1 LEVO MRRCSFASTCRYRSADDVPLGLGSAYVWTCRNITSRGQFNPIHNFSYAMERGVRARDVKAFEKLITNPGPLRVAYTPDYL DWLHRCYKAKGTYMDARAVAEKKFNGNIVSSELSAAVNRREGKRGDTRGDTVDNDHHNLPGAPPPGMFLRPAHSFRRLAG ELKRRRAQSILDEVARAQGMLDLFERQPHFPAIHIDRCSRFHLVELFKEMVLERSLDSNMIWEKALLYRAILSERKPSYP TSFHYIFTAVEDTVFAPTISTPMEMAGSGGEGHDRSSHPLAAKCPTLEAYYYYVYLVKKYYIDNAVEAHVVLRCHREPNA ADLLFSNPPPKDDTEIMKAVELLRNADIQRGVAAAAAVSDPTLPPGGEGSVIGNSDNKKNSEKTSEGSRGRPARPPVLPG AYPPIDMLWRCEENLPLLKVLLFGEFNLIVSENPFVKFPSAHGFLTRPYSTDSSRTLADGMSLANVMAEKRGHLLPSLPR NTATSIDARAQDIRRLQQKHHRDDIVSFQKLLRTTHAEDSPSAFSSYSDWSYFNPRAVRAEERDRLTRKAVEALKLYDSA TNDIYRHSFEDVQACHTQRVTERDRTMPPYLPTLPHFVAIIKKDPHISFLLHIGLPDRNSSEEGSAKHKELEKRIYYLAR ALYHTALEYHNETVRRVNRQKVNVAASLLDNFVEQEWTTILRDKHDVTDVTKTLNDTQNDKKQLARRLGRYMLFANRSLD DTGFPTDARADDYTRWMAPPSVGKVSL Q386Q7 mS53 Trypanosoma brucei brucei 0.075338914 1 LEVO MSVSGVFSKGRGIGHEATTSILRYIPRARVPWQPSRFGRENLTAADMARLWSRGRYRDGPGNYNSGYCTERTHVLEENTV SIIPRRELEKYMPDITIGPKALVTPVSLMNARNGHRVTHDLLHSYDPHIGRLGKPAVVDHDNITVEDPNRVGLNAATLDC RGRIYRWLRRGPFFQVDNYFRRSVKLNRDGTLPTDFVHEAPLMRKIIRLAHRGHLKAACEEYRRVTTVPPVEVYRALTAC CVPGAKLADAVSIFEDGDSKLFYVSRDGEVLHNLMRCAIAARHRARIMWVYNVMRGRFYENVVVRAEVDLIWRYRIAMIA LEYLLDHECAEEAAAIYSYLVEEELLRCDVHVRVGLHMREAIAAGKPITLNNDVMNATSLVRDATAVAPEVARELQRRHA QTLQNNAVEAVGAENVREGSAPWSILGPLTAIGPTAEDTMVWLQQHYGDVDVMSIMRWARFRKGKDLMAKDRPQYLARAA AWIELLSKRNREMEEVPLTYMRKSKPLVLDTNSNVRVAWQTPLMRSGGPPRLLAREEGYVFHHSNSSRFVEETYRHPGES LQSRYLALQPLHTEVSAKEDFQRLYYQAQKHHKQQERLLPVSTAAIPSRIVHHSVMSALHGVSGKGEPANRSLFT(UNK) NKSNDSHSNTGVASGTSACTDGAGSATPEF Q38ET1 mS54 Trypanosoma brucei brucei 0.072046031 1 LEVO MFRRAIPLLSANIPRSVWDPAQHNPNWSDSYGHDITNRRAWPARKWTVGLEPCTPREWLQFSHRNLAYAYNGALRACHSL PSMLLLYKEMKQRGVKVDVDTMNVLLTRAARHEHIQVDDVFLLFDELVALGARPDLAAAETLHTVLSHSASMPEEWREAR RLQLVELYNNLAMEEVERLAPHRADRLLKEQMKRFRGNLQQLGSGLRPTVYCRYLHTTHTAAVLLEEVHNFLWELVPNDH PAMEIPALQLRVPFVASVLRRPSVNPGVSLASVSRAEFGDTDVCAVFLAAAERMVDADFDDQRPVSERRLFLSLLTMISY SGVLYTSDLMAQLMEMVKYSNNDETRDSDAQRVLRYALRGSSAAQDSASRTLWHSVEKVADCRVVGRYIGARNPWNPIRV CFDEQGVFKAYPISTTTTTREVSPPEGNGAVTQEQRASCVEGRTLEALNMRWDDVRRLIECTGVLVTPPSERCPQQQKME VFTGMAVYLRTVATGRRYEGGEDVLSDGAVATSSCEQRRRGTLFAEGYDFDVWVRLFSLVQEVRHDMEKFMADHTLQCVE PEFECWEALLVTLRCALDFCVVQMQGGGARGTEREVVERLFRDVVALREELIEESRTRFGGRMRVLWLQEA Q57ZP8 mS55 Trypanosoma brucei brucei 0.06667667999999999 1 LEVO MLSQNVAKTTVPSYYMIRTNLPHRKPQNQWEGVYYYSGITKRQRHLILLHRKREREAHMRSFNISRASVLQRLEQLSGDR KQESLPPHVRLDLAVRLAQHGLYQQATPIVDELHHQKALHAGHYALLINALACPRLGQRILHCDAQCDPALTYKLLGDEN GEERAQEAYRWFDLALTSLAVDCGGRTQPSHFVPYLPQGTAAASHITNALMRTLLTCGYTHVAAIPDSVYDRMGSMGISP TISTYELVMLALSLQGNMVEAESILSFLRSHHSEHITVESFNALLLGHREARQFDCCDAIWQELVDRRWPRASPLTAELY LRSIMDHANTPTSEPLQSFANINVVEKKKVPLVLAQMDELGVPRTHLSRVLMDEVEDSLRKFQTYRSRFYEWGRAVKQFD FIEFRRRNGWLYDLHLMKCTTKQVGPLRDFNDPDAVQGAVATAEIPAFFNERPAWERPPLEETLYVTTNKERYDDVRGGD IYYDDTRGLHDRSPTWMNEVPETRYDRLYGVNHPDIAKIGIRRHLNVEYVNRKEVVERDAALMKKTLSSGRRLRHRVESS RTHRNAGSLSGISSTAGGGSR Q580V1 mS57 Trypanosoma brucei brucei 0.045956292999999995 1 LEVO MFRSTRPRSVGYTPVNPDTSPMVAYSQYHWHYNLPQGMERPHSVNRTFAAPFQSNHSLVNKYRGVWIEFDMHPAFSVALE PQLRKLPRGRTLPKTPAEEVIADYTALAPLVDDEKTRDLWLAKVFQHCAFQRCGGAMELWERYCHQRFTAEGATAKPPLS LVKSVLFYCNKTDNSGWRALFDRCLKDGWNYTPLFDTAQWSFMLKSIGRMGDEDGVRAVLEEMLDVQADLDRVEARSVVI ALNAVTNADVYEFVKKYLFNFGERKVKFLRTTYSDLRGHGAGKLRIPLKENDNMYYHVCWHSSIRSPRQFSPRQLYFDYT PSTLGSSSHNPNAKIDDIVKDKIEKWKAEGLLPEDYVHEDRVYDRSAAFKNVARQEKWKKMPKILKSKRMGYTGDP Q584U8 mS58 Trypanosoma brucei brucei 0.03562391 1 LEVO MSFRYTNNLIGALKHRLLLESSYREIASRKFIGNCRGVEVVCSGYGTVLAVQLTDKAVWESFYRKGGRPTVSGGGDVSGD AETGTQGSATTTGATGSLDLDKLAESIKTALWDATRKIRSAKEAALHRSLSHNTRMRASADLKHWYEEDANTLRPLAFEA LKHEAATPWMQLVQHGKKEEAAALLKEFEQKGDAAEATPTRVKDDRVKGTRTELSNREQPPLASTLKAEDSNPATIPIGS VHPLFTPALVQIEEAGGGSVSNEAVCRAQLWELSRDEQLFWERVELIRKGQVASIGSSHKRGYADEAAFAKDDTEEKVQL RFTQ Q38BP1 mS67 Trypanosoma brucei brucei 0.030096051 1 LEVO MLVSYLRLQADRRTRHHMMVDGQINPREWFNWHPKEYQPWYFDRHKFLYEQRQYAQFHHLLCHQVYLKDMLHLMRYPEPY THIIDCRTSTQKMHRWVPHSLWLPRDEVEYALQLSADEFLDMYGFRKPDKSDDVILLSHNGIYSEQAGWEWKKQFFQHVY NYRGGTNELFGESYSDMNVSDVLSPWKGPFPQSGVFVDKWSKRKVLTRTGPFDRQYEMQDFALPDLELEKARHPEEGPRQ NMPFGLQ Q586G5 mS69 Trypanosoma brucei brucei 0.021589732 1 LEVO MRRVRDGLFLSHPSSALQCSVAVVSTGEFDHPPFQFRQRHTFNTTPLHDANRFGGRTAYLREIGPVNIKKQGRRFKKDPR TVQFNVDVWCAQQTLRKRWKQRDWEVIEIPFRLVPREQQRVIPELYTDIPQMTDPARNDFSNIRNKVYDREELQGVLFPA AGAMLYPPLQRVDKQAMTLDKYL Q57UZ6 mS70 Trypanosoma brucei brucei 0.021247398 1 LEVO MLRFTHRALTATPERFSVLGTTHPKPKRTGFGRNNKMRSKPSDNVAWYDKGPVEWLPRPVRLTYDHLDQLQQWTMRATLD GRTEEFNRIRDLHREWSQHPLMPVLGDVEPKFPLNLFKQNHRAKKRFLVRWHKANTPANWLWMPRGPTVVTPLHRTNPTQ YPENWKQMVQRKSGTGTPS Q383N9 mS71 Trypanosoma brucei brucei 0.020093226999999998 1 LEVO MFHRAFVSSSDLTGCTIALSSVCTQKRYWAKPKKRPKVGQGFHEKAQKWREEYLLDRHRALADSLRAYVEFSTSKRVEPW DARFKPFDRIEKDGVYVLMRYMMEEKLQLCNYHHRPVKRLFCNIGLMGPQITTKARWKPYRFATNPAGTSKAERMYQRDK TVYTHGHND Q383G5 mS72 Trypanosoma brucei brucei 0.019317293 1 LEVO MLRSTLTSLNTFLTSSVATPPISVIRTGPKWWADPERMVRQKLMYFTLGVDQLPLRRTAVIQRDLQRFHMCKPPPRVGDS TGYKRSRAAQLNTWYRRIQYQEYHMQHLFTRHVWGLLRVYPGNTTKIQGKADDGYVGYDSVPFHRYNRAPLPFPARELYE RRK Q57YD4 uS3m Trypanosoma brucei brucei 0.00907094

mitochondrial gene for uS3m (MURF-5)

1 LEVO MFLIHFVHYKTILQKYTFKFKHIFLSIDKYNSLFFNISGILIWLNIIHINIILIKYSFFILINNFEYLIILIST uS5m Trypanosoma brucei brucei 0.050147355 1 LEVO MFRLTAASQKNLNSWYTKGTMRGGVPRIYYAWMRPGSFTRRRFEKMRNPFVDLETGTSLYFRDTRDSAEAIAHAADSKGI KGMDNAIDLYNEYRIVPDLYPEGFQWKHKLNTEYNQWRSNTWLTPDLIPKEHRGRFLCNFQLNIVAYDMRVVKFSPKDHR QWIYCVLYVGSGKGIAGWGRAVAPSTQEAKKEAIREAFSNIIAVDLEQEGPMYPVRVNADGVRVLLYPARRIVANFRVAD ILCAFGFQHAGCRINLKATNNPKSPTHTVEGVFEAVKALRSVSEIAASRGKVPHSLIYNIYPYLEEIRRRKGMMAMHPPG KDGLLMPDRVVDNRLPDHLKRGYYDDVYWKDFFAGSDEHLNEPRMGLRGDEMRRRLEEAQTSPAPTTAKDTRRRTLEDVL KRLGKTTRDLGSIPIVNPRVDVGLPTHIKRNYQLH Q38AX6 bS6m Trypanosoma brucei brucei 0.018861543

residues 105-114 built as polyalanines

1 LEVO MVFYSFVLVMKPRQRRFTSQALREIGVAVYSNGGLIRSITNEGIMRPYSRFRDADNTPLTYARYIILQLDMGEEEMGKVD KIIREHQDVLMALKLNNLERPVGIRSGNKELQAAYFPLDTFTRLEEEINWSPQTSADIYTQLEMNWKEFSRTRWSSFLRN Q38BW5 uS8m Trypanosoma brucei brucei 0.032766459 1 LEVO MLRQSLLSLAKVYGAVPPLGAAQGHRLLHGKREREGSLFAVANDVKRDERLLRQQLNALLEEERMPMQARLQRNEAVSSH EAVPQTPLVDLPGVERRRDLPADPITRLFFQHKGDHALYYGTYDKPSVQDDDRVQIEKRVPRRREENLYTPIYDFCHRIR EATEQRKRFVVVPSTIETRGCARVMHDHGLVAGFRDFHNDRAFAVELKYFQGDSTINVIEPCSYDGRTEFEWSPKMMRRL LNTHGIHNRLVVYICRTADNRIIDHIHAVKENIGGRGLMMVH Q388R7 uS9m Trypanosoma brucei brucei 0.050201016 1 LEVO MQKLLSPRTARHARLFRLAGKLADSGSPGVPKSDGERLVWVNSHVRRDKDISLSQEEERIRELMMPLEVGENSFAANGQA THGNLFYFREYPMYPGEYVPAEHNTLSSLRDELRLDLTAQSLKEAWMRVSGGVYFQSVDEYYASVDGLDAEQIGEVLAAL FPELNCYEAQALVQRTLECISRPVSAASRQLSRTITAEAVGLDNAPGHYTNFLEWMGRLTETRAFKTEHALFEFSRRKFN RDDVRVMFENYRLMSKATLLADSADSYSHFYTVLKDFARKVAGEDSRHQIGVRIDEAEVDPETGIAVGRGCADGEKYHFT ALLRENRDHNGIITVMGKPLSLVLDNKAWLMEMVLMPFDEANLDYRDFDAHIVSEGHAMPSIANEIAAFALRMAVANALV KLIPLTRIPLKKSGLLSVDRRRERGQFPGYLDGKKVKRRFAKR Q57W62 uS10m Trypanosoma brucei brucei 0.094351039 1 LEVO MVRRSHVVAYYWSRYRMPTQMPKFDGPAPVAAPQSMNSTKTNEFIDPIDDKFPMSIRGPLVRPDVPEDQYVDSWYICTSM THHMGDYRPWSASAPPNAFRFRPFNEFDAKGREYVQYMREFARFDPRKSRGNGQKGFPFRDAYLTKMNEANQKTPPPTLE TIMDRAVREHHQHARILSPLEVQRDVGRLEPIPSYAGKINADRSVFPFQWKTEDWYEYEVAKVRNRRFVFENTEEDGIRG SEVTYKIVLEGFWDHHVMKLAEDVCMFLKDVGRQIVEEKLVAVRRLLQGGAVDPELLAAFNCARAGPFGGLDEYDKEEVA NFLRSDLRRLEEQCLSVINRCNVPVPGATNIYDPHTSWPHVEKLEPWVRMAEFWTSSSDTSFTELEMSTAHYEFRKFFRV IICKLPFQSTEFEKRMYDIRHWLHRQTSCEFHTIYRRNVIHDSAVFPTEHDPATPTTHEHHRMFSFALDWQSAPVNRLST DTVHEGESWDAVAQRLGCSVGELKDANAERETIEAGVVINVPVTATRRLTSFGATPLVLPLKTTSAKDGERIRTWEEAAA ILDCTVEELQQCNGHAALTYQKKESEAGEFDSSETELVAPLSCWTSTSESEFSPVERVHANDTLVAIARRLQCSEEALRA VNDGITDVSGLDFVRVPPEARRPRRLVEPQLRPQAATDALLARTIAEEETFKLKSIPHLPQNAERFPHEYHTPTSRFPPT PSETPATQDWMAYTAKYLDKQFTISAEPAPVYNVNKLWPMQQIPGKVDQTPFEEDQTWLLHSIPVQQLEMHHHEKDLQDL PFINHEQFPRSLEWNAP Q57Z45 uS11m Trypanosoma brucei brucei 0.03792591 1 LEVO MMRKSVTFLRRGKPRPRAGMFPDKYRRVPMLLKPQQGGQQYFNHFLIRSTNDRLTQQDVDNASGQAHSFISPQLPQMDWR NMSARSSEESIREEMRQLAENDVMQHQRVFNERMWYEKEEEHRMKARSCPEETSEHA(UNK)SNDGAVPPPRVLGGDYFK TRFGYSLVKNSEMTQGPVDYSQLDMWGEMPRYTSDMVFLYLVSRRRNTYAVAYTYEGKRILNTYTAGNRGLKGGDRGFRS EGSTDNGHQVTSMYLNDLLPKLREMRASEGRPMGRGEKVELVVRVMGFYNGRQGAVRAVQDRANEFHVRYFEDITPFPLN GPKMPRGVFK Q389T7 uS12m Trypanosoma brucei brucei 0.010421635

Mitochondrial gene subject to RNA editingalternative start codon

1 LEVO LFFLRMWFLYGCCLRFVLFVLCYYMSPRLPSSGNRRVLYAVFYLYNFVWMLRCFFCCFIGLVMSLFIIEGGGFVDLPGVK YYTRIVS uS14m Trypanosoma brucei brucei 0.019900013999999997 1 LEVO MFCFSRGLWMRHIGQDVPKRHTHFVLESRLMYEKSFRDCWLHSVCRAISQLDEPLSKTVVGTHQKMLQRKVTCFQYNQYG LFKTPYYRLANVDRYHAVQGVAGTREWVPYVNVSYWTMNKMVRGGNLLVHRVHYTGWGTDSHLKKGGWEHRWNKVLQRNV LQYSRI Q580I0 uS15m Trypanosoma brucei brucei 0.050401191 1 LEVO MFVRSFCFLRKYGSKSKTPDLKTSFTKTTNFRKAEKISITTTDGEIVKGRGKIRRRTHSALKEAIGAMEHPAIWLWYPWR MNPEPPTPHMPQRRALKNVHGAVFNDLTPVQKKRQEQMLYGVNIPETRQMKFEEQHPLLAGALRKLEGQPKGFPFWYRKY PTRRHAYEYRFSIPVEMLDGYNDDVKKALSKGMMSIQEKQFAQEAMYMERYAEHDFDTTSPAVLAVKRALKCRVLRNHLL TNPHNNIIKTVLANTERKLNHALRRLRKVDFKKYWEIIRDHDVQDILQPPNLVTYRQGSYWKYDWNAGLAISTNLADVMD PRGLNGCVETGRSRSEVARDLGLSYTRPLHENEKKQLSHQAVYYERLAKFKMEQPEAARAMERERFVRKFSGMFVKMDIR SGAPDFPSTYRRLLGTKVVRWASKRHGPN Q4GZ99 bS16m Trypanosoma brucei brucei 0.022077805 1 LEVO MQVTETVLARAVIKRRSPQLWGAPGAPIIRMRGHHVVWKFQSYDLVVEHTHKRRNSDIRLLHYLGKHCPHPQKSLWSPDT PVAQDRHLFMLTTVDIDAFKYWFGVKRCRLSMKPWALLAKAGLLPPSLTQNSKIMPKPLFDKESLMRYYLANRKDEDVMA REKYLNYENSMVKTEEERAAERPVAPYL Q384N9 uS17m Trypanosoma brucei brucei 0.035632726999999996 1 LEVO MLRRSLLAFPWWNFQTEHRQRCVLMYGGARTKNTHNANHRVFIKKYKRNAFPNRTRHHWAVSMTGVLSQRPRRMPWPYDL TSLIFNQPRQGSDKIGYVVGTSMLKTAVVATNHMVYYPKFNQRVSRTKRFFAHDEDLACVEGDLVHIKQCRKISKYKHYY VFSILEPNVEGRERLKLGLKAVPPPLFGYPVSRRIVKLNLTSTEGTQEKLAAAIQEHVQDAYRFSGPTPDQPRNRLADPV TFEDANNMIAPNAPAAAALDASDSPPLLDRGEYTEVEQDTRNKKGDDYWMNLQPKEKYDFKSFKKSP Q38DP8 bS18m Trypanosoma brucei brucei 0.036988691 1 LEVO MNRTGGSIYAHALSQFAVCRQPWNEYIGLLTKQDSTPYHTEPQEKPAYRGRKQGREGWLFGQQVQLHYHRFPDEQLITNL SRWRTGETVGDIAMQQFRNAQPFDIEDKDPQGVQRPSPEVYMKLNYKNPATISRFLTRTGHMYPADILPLNPEAVVKLRV AKAQAIRIGLYPRFGNPFWFRSQKFRPKAYQENYDPTTYSTKRTVEHFAYNWVQTDRIRRYFRELEAVHTSGSASARGSG GGTTAEHKQQDQFYSPENQPISLHRNNISYMEDVGRSVKNPTVPGLMSTKGMKKKFHNLYSSTSTKRMGFSNPTLGIKKV Q38AS2 uS19m Trypanosoma brucei brucei 0.028364603999999998 1 LEVO MAFRNTFTTPGKFSTVSKNIVLLLIWRVKVFLRAEGFAHSLVMLPVSLYSKILLCDVKKKIVYFHCCTRKKSMLRRCPCV WPDGPTKSSVSIGTAFTLQKRFLKIAKSAFGFYLARRGQRKYPFLRRPHIKNTHSMNPSAPYFWSFMTAKSQMAFLPEEN YITGDWTGKFFVSKRQVYTLQHATSGAKVRVKSFPSIFEFNSPSRWNIGKEMNTLTKPRMDLIDEQMLTKKQRLDYVRAG LLPK Q584T8 bS21m Trypanosoma brucei brucei 0.023087888999999997 1 LEVO MLHTTRLWLGGYMMYHRKAMGTMKYSKWKGAHGGISHFYGRTPMVEEVRPNEPITLVDRRIMHYVHHSRLRHFQLFRSYQ EKSNSTECKLREGEMLRRRWHRRLQKSFIAFMQFKTMKVLEDQARLVNTYGQAAVNAALGDPWNATDNVARERKSAAVRR QVRALPMVNVVPKHVATMKQIHNDRFNYRWRVN Q580M9 mt-IF-3 Trypanosoma brucei brucei 0.041086285

residues 294-304 built as polyalanines

1 LEVO MMQRCSTSLSRLCFRRLLRTPLLVYSIPPTRDVPSGIAHCPLSCSMRMVTSSNDDEFVFDPTLSIQKDAAIHTAKKSFET IVLEYVPAHAPEEARQKVKSYLTQHPIDILITQPKVQITHLEDAESGAETKVSLSPCDLPEALQQARERGMNLVQMGARG DVAYCRIRRESTRILSLIHTELEALREQEEKQQGKGRGGVQAAAKMGELIDHTFRDAVDAHFVGWRSKKIVEDIRRRHPV KLTIKEFQSPECAIGKLREMCQAMQHYAQEKVIYHHFTSIVANDREASITFAPALPMAKSDSWKHIKYPGEKEWTNALRR MEDACRKSGRYGTYAKSNKLKLRSLGQTSYRVDKYGRKMD Q57WU2 mS22 Trypanosoma brucei brucei 0.073064141

residues 20-37 built as polyalanines

1 LEVO MLRRAYIQRRYPFNKRGPREHKSWKHHVLTEPPKPLQWRDPKVWTRDLSVMKSFDAPQWDLWQSRPRSEDMDEALQPFMD MPKSLKDRRYDIPWWANPFGAWYLQNILSLELLKLKSKTNAEKIATYRSYMRSLASGKDNTMSDDDVIRNIIKERWKTLE FGDRNAGYPCTFGDYIQFLNEWFKSLDEEGMQRLREHFDRRIRPLLAVMSPVDILWLEALTQNSPHNKEQLQRKIAFQTS LGTPEFFDMSKRLRYEINEDYKVRDELGPELFALWSKAPERWPPERLSKMYGLDFTLVRKILVWHHFKACYDACVEPDWS LPKRLFALEWIRDVRARKHGLFYGKMRFAEQKITFYSDRFLFRDLVNRREASYANVWEMDDPYRFLQTEQDYEDYWGDNY DVYRRMFPEMIGRTGEPVQQYGQMPIWAGPHRQHANKSEHNWMFAEIGVNVGHEALKKLELDPTNEKRRRFVIRQPDGTL RSAKMSEMRAWYWKEEWADFRFWAPQMEWGIENTPSQEQYQEHVPDTTDADFRKQRRIQSRPVKWFYESHYTRTGSFAGF QPLRFMQRRTEREVRWPDVINAAVQIQKRKPAAYIFKAIPEL Q38DR3 mS23 Trypanosoma brucei brucei 0.036855578 1 LEVO MRQCVVRRYKMPKNMGVAPRFDTWNEKYEPWEHMKRMGRLVGTGFYIPPEWYNHFRMFPPINHNFQQEKTLNPHNASEPT QDDTSTLSPERVALRDELARKSRLVASEGMRYYNIFWVRKPLDTMEKEYYELKRRGVDHGEAIRKVLQGFYSGLAVKKRV AAIQAEEAKLTGRFITMREATVVLGVLAKLHKEQLTPHQVSLLAKEQGETTQSGAKLTAIVSRTQPHVNKEASSPATSEA VGSSTEESLSADALASMLSEDGEQSAVGTRYQVEVKETANDSVRQLREKAEDQTGFPDWYTGESPTYSGTSACSRDGFAL MKANK Q57VB2 mS26 Trypanosoma brucei brucei 0.051164737999999994

residues 221-248 built as polyalanines

1 LEVO MMRSSSFCRRQIRPYYNLPSKSEHGRKMTGFLTPYRHWMWKQNELWRNVHEAQFEHLRRVYKRQWLESFRVNADEYIYKY NITKAAQLAQWECEMKEQEKKRIEARQMMDGRQALKKKHLDLLREFHERQFFFWYERASERLQNMNLINYVPHAQLREHI DKELDKYVAGKNEPYPLNFVGQMPFLEDGDGNIVEVPESLLSNHMAEHPDSTAKPHEPHTSSSISEAAAFEERMLRAMVS AKEEDLKEWLGDDSRALSETIDDISREEEEREADIRVARSMEETDAEREVSRRAYIER(UNK)KTGSRSIFRPPTVSEGA GGTPSAPAGDANTPMRRRKKGKLDRVHALQAHQDELLAKLSSQGLKEGVDASSVPERGKIVQSRGRIRDKAVIPTHEVLM QKPELAAGSTPGARIQTKDMVDKMYHRGKYKKSGSGDKSDGEDL Q38DK6 mS29 Trypanosoma brucei brucei 0.057048836 1 LEVO MRSKTLKKSLAAMYMRPPVTCYTDACEAPVAMWDGAIPLKETRKLKNGVPVRTVSRTYSHPPQLTPTQLSFNDINSMYCV GNDELIQFFPEGLGGRVFQTMPPGHPRGFLYRKETHLLNLFVDKVQHWHTKRSVLSSLTNGRTGFIVDGPTGCGKSALMC QVVHFARSRNIVTLYVPDAKVWTHGEWCWPSTILPGFFDAPDAARSFLKYFAVANRATLTSWKLRCTPKDLPTEQGERQP QNLYELCEWGHRAVAPASIDRQSVCVKFLMDELSEEKKLPVVIVVDGWNLFSHETHFRYPHPDFLRGLASFNESSTDIDL YPQELPRIPASRLSFVRGLNKMILSGDDPNKFFITCTTRDFKPFDGISGFPNVETDRFANSLDEYAPYDPEKDSHFHPIQ IGNFDEYEYRSFLRFLINSGELAGLGWGPLWHASSDFERKLYKIGFLSGRNPQGVVDHYHQELVWRYDYQRTRQKQYLLK RRMEGMSRGASSGAVGVR Q585C2 mS33 Trypanosoma brucei brucei 0.021244104 1 LEVO MVLRRWFPLLGVHRVGYTHPSTLPVPCAQRWDLRLARARIFQEYIEEKAPGAWQLEDERSMSPEFKTFTGYPMRDMRPGY GQNLPDYIMKKRLPNNTHYELFARRDIPNEDNAMYGKYLYDMTVHGTSLPSTYRMHKDINKAQRNDRKLSGNRFKVLCSS GAKNPPSGWEPIPDATEEEED Q57WW0 mS34 Trypanosoma brucei brucei 0.02872818 1 LEVO MLRCARVALRADPLNGGSSMTLGSKGSKLSPEPHRRRMPWTAAKEYVPGVVLNARDKMVLDGVQLLDIESIDRASQLDPL EVLRAVVATREYNISTGKNIFQLASQATYNGRGQRFYRKEWQEGTYDKYVTLSAIDFDRDGNKGTAYGYITFHGETTTRP VQVDFADVPGWYMDFVEERAVPFTGIVPPPPSIGTDVPVDPHSYRLKAYPYYDAPNPPEFVERLLKDRGVLPDTPTETAD VDKDPTTSDGSVHYDGK Q57UK0 mS35 Trypanosoma brucei brucei 0.098377773 1 LEVO MRRWHRSLNPALFGERGSGHYFSTTRARLQLQAQDRIICGTHGAGIVAHQTAVNSSDNPMLSSVTTAAPAADLSRTKPHE GTGTSERDPYIRTLHNQRSAAPESSVSQSHTVNAPTVDECEMLAERWGTMNYWHNDTFPRLVVFLKKLLVPDVSPLSPTA ESLLSMFEKVVIPKLTSDEEDRRKLVSLWSETTLQAEAAVTKFLFQRGSFESMLHRIITDALEKMSTLALGGQEGNLALE ALKRQTLFKRNDYIQKRLIDVVSNSAYLGYGDSVWQVFFAAVEANEENLLSDRATTDAIRAAWEGVMREDVVRLPDVTGV VALYLTLVCIRESGRLVPEELKELSSGLEDGVRPGVRKLQQYPLIFLHPTVKRRFVVKAVAEILHNSSSNAFSNMLRENG LHDTAREVALCEAMNRNKELAEGDVGDAVGRFVSKGEVKTLLSSLVSGTDAVVRDAVAGIFGIGTTITIDWDAVMQNVDW SNNWQRLATALLSNSAVLSAIVKLVKNAIGAKGMSKHLFTDEYADQLQLILDAREERAASRKQRIENIAQELSSFERVDL SCDLLRKLGVDMTELDTAAAATRNMNVVQRPCIEDGLLSLVLEAVTKRHPNWVKAGVIQTTLKDPFDALRWMMHIFIRLS YVPHAGAATIARLSRRRIGPIGLEPHQFNVPAELGFVEQYDNLQYKRYDWQGWYQRMLDVHNRNVSLRCRICDLQRLDGN GVQFVDMQTERRLRILAQHRVGMGVLKLDADKYEDQADNVTFGTTKLSELLADARKAQLGEEYWPSVELKVRKPSGQSKA HYSLIDNERIEKRSRELYEKYRDAKKRSLFVTPMETWLEVKGMQVRKSVDNADEDGYTLDALQDMMDGDDGDKV Q387C7 mS37 Trypanosoma brucei brucei 0.024757895999999998 1 LEVO MKSSDIFFAYRLTPVVFKSRQHDSGVNQYGLKPTNAYDYINPTNLINFGRGTTFDNLGVRRAGRGEIDSSPSHSGSPVFT QAKLIGLSGEEQLTMCQSETMALRLCMAKAGKETCERESRALDSCLGRVGHLRRAMSEACWEFNDWFIQNVSDNHTKPFQ HRPHDWRHFYAQEKLVRERQQNGHAYGRRPKQFSFGARYVKTDGYGKRPRLPYNK Q38C96 mS38 Trypanosoma brucei brucei 0.028240599999999998 1 LEVO MRAGCVACSRIPKLGIAALGSTTTSMQATCQETQELRCATTQMAPAVIFPLPPPLRGSYIDRKPTAASFNEHHATASFRH HMIASADVSHRPRHFYFASGTRNDTGTRSVKEGERKQLKQQTNVEVDSVAYGDQLDELSARWAAKFYGQVTFGPRNYPYP SSRWLARRFQMKKHRIIKRFRFRRYKLAAVANLPFAKMIRVGMLPELKSSKTKRGDVVDPTLSGQLVSAVKNTGEKRKGQ RTRPKSKYQV Q57VQ9 mS41 Trypanosoma brucei brucei 0.022421469 1 LEVO MLKRTLTVLDQTYGPHKSYKYTYMPDPRKLAPIETTQRSEIVPQSIRPPTSYVPNHETFLERADIHRLRPTSDFKASFKD WNDLFTCDKRQLRVRGIPRMTRDAIRTAVQAFQNGNPPERFDTKEEWLYYKQFKTVDYSYRVIPELPEKYRPHQSGIDQA PLPDYREINKMPQWAECEERRQSKKTV Q389L3 mS42 Trypanosoma brucei brucei 0.029748666 1 LEVO MRLCRRLSYHAYVPKGASVQMGETFMPSNIATAVFRETEAAAARADKTKEETGFFSQPRLNYPVSSGIPALFSKDQFDMQ YSLFHRDVVETLNRHTLGTPLEGHNLETVIRKTSFDATQAVAHTAASEHFNYCFFYKSLRPWGTAPPPRLREALQLQYGT NGSADAMDEVRRIVWVTAASHQERCGWVYLVWSGVHFDVVEFPHGSCPIASDLIPLLALNVHESAYILDYGTSGLKQYVE NYFKACNWPLAERYYLMAIGQVV Q586A1 1.15.1.1 mS43 Trypanosoma brucei brucei 0.04859041

residues 27-35 built as polyalanines

1 LEVO MLRRTVTSLRNARELHRLLPALLPLLDPPVPTGASVCAAHLIVATGHVSKQREPHMSNCPVLERKIPLSEIASDDNFLKH PCVPCLKHRINFLASEDFCYDFVERCGCGTDGALPPEWRCIVERQFGSMAALVEELVIHASNRREAGWTWLVYDAQQAGE RPLLVVNMPAQRSPLLLGLWPLAVVNVTEAVLVEAVRAESSGSVAAAPPPPWCRAARASLYDTKAAAEAFAMTAPMSEGV GKKLSEIREKVARDAVSRMNWGFILQQLKKAETYYASSDRGEKIRVHRMKLEREAVTKAMSGLRDSGATFLTHDTVEVTT TPSANSEPSSPERVPGVQAKQPQTEGMDPCKNKASAEPDPKCTQLPDGTWQYSYRNGDVTLLKPDGTRVFRKRELTTTAY PDGSMLFQYANNTSILDRADGVRVTTFEDGTTKEEMIRR Q585I1 mS47 Trypanosoma brucei brucei 0.137928969 1 LEVO MERRRLLHDGRWFTPPVLYGSARRGLKTLDVREYRPLAMPIEFRFYQRYANHPNRQSGIQFLTHYNTHQRFRVNKDYIDY MHWGKEQGQARLPHRHQRVAFDFNDQLHPTRIGTPEDGGNANHCYPEDGEDGSYAWFASQDPTFGHHPDLSSSFDPNYRV FSHPEHWNKMFTKRRPGEGDIDLSVLPSRSLLGPLMAHSDTKGIPYFKVDNRGHSNGRVPGVNAPFFGEFDQKMMQAMSR PLNANRTITGNDGRFSKTIMINEPQCNQALSAKTASELSKEIDKATNAVYSKLSVLEAAQSGLTDYFCGGLNFEIVGFEL HMAAMLRERAAAILERSGAGGLPSASSATLSSSSTTRRCAMLLEKADEREVNKLLRDASRYEDRVDDALRQHASLIWRVY TAPRPLMTLTNGKCRGTGCGLSLFAKYCALKDSSEFIFDGPNVGITPYGGMTRLLARPETSLKYPGLAEFVVLTGASLFA GDALRLGWTDLFTTIPDMTYHIKEWFDTTEHMHNDAVAWQLGHLLETCFKMKESHSSAMERAAITPTRARWVEDAFADQP SVNDIMRTLTEIEQLPFQSTHNTSDESHITPYTLGSVAAGVQRLGEHRLRYTLSPWDITPPEDSVVLKHTSEMFRAYVLE RRGATDVVTHRDAERAAAWSQQRQREYDAYCSLKEAPHPRHVYARLEGCEGKIVSFEFVFEISCRRSGPQGTDEVLQTTT ISRLKSRILDALGMPRDRNIDLGWYLPTLDTCPIHSDEELMQVLHTDPGIEDPKEKLRYPPIYFIVKRCTLYFSEWAYAV KHQLLLQSPFALRAAFGLLQEVRGDGAAENVMPLAETLGTEFKYFTRLMRRPDFYRVGVHTDKSVEKWEEIKEERRHNIH HTHRPTRPLPDFEEVFERDVEIDGHRFVLRPRWNPRTLQDVDDSDILRLRAPLTYDMDSTAALHVPTHCGKASRLAGMLQ DAGGVQVLDGLGEVDDKGEPKVPPLQSNASVPTNVSFYEMARHPWEDRASSWRRDGFTEGSLEYFESQYRSAERAVYDEE GRGVRNYWPSRDAVEGIAQEEENDAQLLQERLFNTLETAGTSVEPWARNLRLNATTGKFGYKTEIATQEEKIYDDEYYRW FIQPGRHPNPSGLTLNGRKGQFEEGHSRDEELERMWRKVVADGNNDFAATTSGMPVYDEDAKNAEDSYAAGDDVDVVTTD ANVPVDGDDSS Q383R4 9S rRNA Trypanosoma brucei brucei 0.198442391

post-transcriptional addition of 7 U residues at the 3' end

1 UAAAUUAUGGUCAAUUGUUAGUAUUCAUAUUAAUUUUUUUAAAUGUUUUAUCAUUUUAUAAAGGUUUAUUUUUGAAAGAU UUUUUGUAUAAAAUUUUAGGAAUAGUUAAUAAUAAUUUAUAAUUUUGAUUAGAUUGUUUUGUUAAUGCUAUUAGAUGGGU GUGGAAAAAUAAAAAAAAUAAUUAAUAUAUAUCAAUAAUAAAUUAAAUUAAUCUAUUAGUCAGAAAUGGAUGCCAGCCGU UGCGGUAAUUUCUAUGCUUUUAAAUAUUAUACAAUUAUCAUAUUAAAUUGUUAAGUGUUGAUUUAACCAAUAAAAAUAUA AAUAAUUUUUAUUUGUUUUUAAACACCAUUAGGUAUAUGCAAAUAUAAAAUUAUAGUAAUUAUAAAUUAUAUUAUAUUAU AUUUAUUCAUAUAAUUAAUAGGAUAAUAUUUGUAGUUUUUGAUACCAUGAUAAGGAUUAUAAAUUGAAAGUGUUAAUAUC AUAAUCAAAAUUUAUUAUUUAUAUUAAAUAUGUAUGUGUAGAUAAAAUAAGAAAUUAAAAAGGUAUUGUUGCCCACCAAU UUUUAUAAUAAAAAUAACGUGCAGUAAUUAAUAUAUUUAUAAAAAUAUAUUUUUUUUUUUU M94286.1 Unknown Protein Trypanosoma brucei brucei 0.00044353899999999996

residues built as UNK

1 LEVO (UNK)(UNK)(UNK)(UNK)(UNK) Unknown Protein Trypanosoma brucei brucei 0.000613749

residues built as UNK

1 LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) Unknown Protein Trypanosoma brucei brucei 0.00274137

residues built as UNK

1 LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) Unknown Protein Trypanosoma brucei brucei 0.0006988540000000001

residues built as UNK

1 LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) Unknown Protein Trypanosoma brucei brucei 0.0046136779999999995 1 LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) Unknown Protein Trypanosoma brucei brucei 0.0037626289999999995 1 LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) ZINC ION 6.5409e-05 4 ZN URIDINE 5'-TRIPHOSPHATE 0.000484141 1 UTP MAGNESIUM ION 2.4305e-05 39 MG GUANOSINE-5'-TRIPHOSPHATE 0.0005231799999999999 1 GTP SPERMIDINE 0.000145246 4 SPD SPERMINE 0.00020234 1 SPM water 1.8015e-05 3 HOH singleParticle particle 7.4 ETHANE-PROPANE 98 278 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI FALCON III (4k x 4k) INTEGRATING 40.0 1

initial model from 2D class averages in EMAN

1 FOURIER SPACE 3.37 FSC 0.143 CUT-OFF 31911 OTHER MAXIMUM LIKELIHOOD RELION 2 RELION 2 AB INITIO MODEL

We used Coot and O for initial model building and refined the structure using PHENIX

46.399999999999999 emd_0230_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 444.8 444.8 444.8 90.0 90.0 90.0 X Y Z -0.042286687 0.10807089 0.00052695617 0.008326632 1.39 1.39 1.39 AUTHOR ::::EMDATABANK.org::::EMD-0230:::: half map (odd) of the T. brucei mitoribosome small subunit in complex with mt-IF3 emd_0230_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 444.8 444.8 444.8 90.0 90.0 90.0 X Y Z -0.04088965 0.108935796 0.000527337 0.008317952 1.39 1.39 1.39 AUTHOR ::::EMDATABANK.org::::EMD-0230:::: half map (even) of the T. brucei mitoribosome small subunit in complex with mt-IF3