2024-11-20 REL PDBe PDBe PDBe 2018-07-01 2018-08-22 2018-12-05 2024-11-20 Medical Research Council (United Kingdom) MRC_UP_1201/6 United Kingdom Cancer Research UK C576/A14109 United Kingdom Yan K Zhang Z Complex, DNA BINDING PROTEIN Yan K Zhang Z Yang J McLaughlin SH Barford D Nat. Struct. Mol. Biol. US 25 1103 1110 2018 30478265 doi:10.1038/s41594-018-0154-1 1545-9985 EMD-0095 other EM volume EMD-0097 other EM volume 6gys FULLOVERLAP Cryo-EM structure of the CBF3-CEN3 complex of the budding yeast kinetochore Cryo-EM structure of the CBF3-CEN3 complex of the budding yeast kinetochore 0 1 2 3 4 5 6 Saccharomyces cerevisiae Centromere DNA-binding protein complex CBF3 subunit C Saccharomyces cerevisiae 0.056416863 2 unidentified baculovirus LEVO MPSFNPVRFLELPIDIRKEVYFHLDGNFCGAHPYPIDILYKSNDVELPGKPSYKRSKRSKKLLRYMYPVFATYLNIFEYS PQLIEKWLEYAFWLRYDCLVLDCFKVNHLYDGTLIDALEWTYLDNELRLAYFNKASMLEVWYTFKEYKKWVIDSVAFDEL DLLNVSNIQFNIDNLTPQLVDKCLSILEQKDLFATIGEVQFGQDEEVGEEKDVDVSGANSDENSSPSSTIKNKKRSASKR SHSDNGNVGATHNQLTSISVIRTIRSMESMKSLRKITVRGEKLYELLINFHGFRDNPGKTISYIVKRRINEIRLSRMNQI SRTGLADFTRWDNLQKLVLSRVAYIDLNSIVFPKNFKSLTMKRVSKIKWWNIEENILKELKVDKRTFKSLYIKEDDSKFT KFFNLRHTRIKELDKSEINQITYLRCQAIVWLSFRTLNHIKLQNVSEVFNNIIVPRALFDSKRVEIYRCEKISQVLVI P35203 Centromere DNA-binding protein complex CBF3 subunit B Saccharomyces cerevisiae 0.071439891

Zn

4 unidentified baculovirus LEVO MFNRTTQLKSKHPCSVCTRRKVKCDRMIPCGNCRKRGQDSECMKSTKLITASSSKEYLPDLLLFWQNYEYWITNIGLYKT KQRDLTRTPANLDTDTEECMFWMNYLQKDQSFQLMNFAMENLGALYFGSIGDISELYLRVEQYWDRRADKNHSVDGKYWD ALIWSVFTMCIYYMPVEKLAEIFSVYPLHEYLGSNKRLNWEDGMQLVMCQNFARCSLFQLKQCDFMAHPDIRLVQAYLIL ATTTFPYDEPLLANSLLTQCIHTFKNFHVDDFRPLLNDDPVESIAKVTLGRIFYRLCGCDYLQSGPRKPIALHTEVSSLL QHAAYLQDLPNVDVYREENSTEVLYWKIISLDRDLDQYLNKSSKPPLKTLDAIRRELDIFQYKVDSLEEDFRSNNSRFQK FIALFQISTVSWKLFKMYLIYYDTADSLLKVIHYSKVIISLIVNNFHAKSEFFNRHPMVMQTITRVVSFISFYQIFVESA AVKQLLVDLTELTANLPTIFGSKLDKLVYLTERLSKLKLLWDKVQLLDSGDSFYHPVFKILQNDIKIIELKNDEMFSLIK GLGSLVPLNKLRQESLLEEEDENNTEPSDFRTIVEEFQSEYNISDILS P40969 Suppressor of kinetochore protein 1 Saccharomyces cerevisiae 0.02235727 2 unidentified baculovirus LEVO MVTSNVVLVSGEGERFTVDKKIAERSLLLKNYLNDMHDSNLQNNSDSESDSDSETNHKSKDNNNGDDDDEDDDEIVMPVP NVRSSVLQKVIEWAEHHRDSNFPDEDDDDSRKSAPVDSWDREFLKVDQEMLYEIILAANYLNIKPLLDAGCKVVAEMIRG RSPEEIRRTFNIVNDFTPEEEAAIRRENEWAEDR P52286 Centromere DNA-binding protein complex CBF3 subunit A Saccharomyces cerevisiae 0.112066031 2 unidentified baculovirus LEVO MRSSILFLLKLMKIMDVQQQQEAMSSEDRFQELVDSLKPRTAHQYKTYYTKYIQWCQLNQIIPTPEDNSVNSVPYKDLPI SAELIHWFLLDTLITDDKPGEKREETEDLDEEEENSFKIATLKKIIGSLNFLSKLCKVHENPNANIDTKYLESVTKLHTH WIDSQKAITTNETNNTNTQVLCPPLLKVSLNLWNPETNHLSEKFFKTCSEKLRFLVDFQLRSYLNLSFEERSKIRFGSLK LGKRDRDAIIYHKVTHSAEKKDTPGHHQLLALLPQDCPFICPQTTLAAYLYLRFYGIPSVSKGDGFPNLNADENGSLLQD IPILRGKSLTTYPREETFSNYYTTVFRYCHLPYKRREYFNKCNLVYPTWDEDTFRTFFNEENHGNWLEQPEAFAFPDKIP FDFKKIMNFKSPYTSYSTNAKKDPFPPPKDLLVQIFPEIDEYKRHDYEGLSQNSRDFLDLMEVLRERFLSNLPWIYKFFP NHDIFQDPIFGNSDFQSYFNDKTIHSKGSPILSFDILPGFNKIYKNKTNFYSLLIERPSQLTFASSHNPDTHPTQKQESE GPLQMSQLDTTQLNELLKQQSFEYVQFQTLSNFQILLSVFNKIFEKLEMKKSSRGYILHQLNLFKITLDERIKKSKIDDA DKFIRDNQPIKKEENIVNEDGPNTSRRTKRPKQIRLLSIADSSDESSTEDSNVFKKDGESIEDGAYGENEDENDSEMQEQ LKSMINELINSKISTFLRDQMDQFELKINALLDKILEEKVTRIIEQKLGSHTGKFSTLKRPQLYMTEEHNVGFDMEVPKK LRTSGKYAETVKDNDDHQAMSTTASPSPEQDQEAKSYTDEQEFMLDKSIDSIEGIILEWFTPNAKYANQCVHSMNKSGNK SWRANCEALYKERKSIVEFYIYLVNHESLDRYKAVDICEKLRDQNEGSFSRLAKFLRKWRHDHQNSFDGLLVYLSN P32504 DNA (52-MER) Saccharomyces cerevisiae 0.016168495999999997 1 (DT)(DT)(DA)(DA)(DA)(DA)(DT)(DA)(DT)(DT)(DA)(DG)(DT)(DG)(DT)(DA)(DT)(DT)(DT)(DG) (DA)(DT)(DT)(DT)(DC)(DC)(DG)(DA)(DA)(DA)(DG)(DT)(DT)(DA)(DA)(DA)(DA)(DA)(DA)(DG) (DA)(DA)(DA)(DT)(DA)(DG)(DT)(DA)(DA)(DG)(DA)(DA) DNA DNA (52-MER) Saccharomyces cerevisiae 0.015856242 1 (DT)(DT)(DC)(DT)(DT)(DA)(DC)(DT)(DA)(DT)(DT)(DT)(DC)(DT)(DT)(DT)(DT)(DT)(DT)(DA) (DA)(DC)(DT)(DT)(DT)(DC)(DG)(DG)(DA)(DA)(DA)(DT)(DC)(DA)(DA)(DA)(DT)(DA)(DC)(DA) (DC)(DT)(DA)(DA)(DT)(DA)(DT)(DT)(DT)(DT)(DA)(DA) DNA ZINC ION Saccharomyces cerevisiae 6.5409e-05 8 ZN singleParticle particle 8.0 ETHANE FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI FALCON III (4k x 4k) COUNTING 27.0 1

Initial model was from SIMPLE_PRIME reconstruction.

4.4 FSC 0.143 CUT-OFF 22668 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD 6GYP PDB experimental model FLEXIBLE FIT REAL