2021-10-20 REL PDBe PDBe PDBe 2018-06-04 2018-08-22 2019-06-19 2021-10-20 Research Foundation - Flanders IWT-Flanders Belgium Research Foundation - Flanders GOA Belgium German Research Foundation Sofja Kovalevskaja Award Germany Negative stain EM map of the inner membrane protein GspF (XcpS) of the bacterial type II secretion system Van Putte W Savvides S Van Putte W Savvides S Kudryashev M De Vos T The inner membrane protein GspF of the bacterial type II secretion system adopts a dimeric structure to mediate pseudopilus biogenesis To Be Published 0353 EMD-0045 associated EM volume fusion protein of the His-HaloTag and XcpS fusion protein of the His-HaloTag and XcpS 0 1 Pseudomonas aeruginosa Escherichia coli 0.150 His-HaloTag-XcpS Pseudomonas aeruginosa Escherichia coli LEVO MAHHHHHHGS EIGTGFPFDP HYVEVLGERM HYVDVGPRDG TPVLFLHGNP TSSYVWRNII PHVAPTHRCI APDLIGMGKS DKPDLGYFFD DHVRFMDAFI EALGLEEVVL VIHDWGSALG FHWAKRNPER VKGIAFMEFI RPIPTWDEWP EFARETFQAF RTTDVGRKLI IDQNVFIEGT LPMGVVRPLT EVEMDHYREP FLNPVDREPL WRFPNELPIA GEPANIVALV EEYMDWLHQS PVPKLLFWGT PGVLIPPAEA ARLAKSLPNC KAVDIGPGLN LLQEDNPDLI GSEIARWLST LGSSGLEVLF QGPGLSARDL ALVTRQLATL VQAALPIEEA LRAAAAQSTS QRIQSMLLAV RAKVLEGHSL AGSLREFPTA FPELYRATVA AGEHAGHLGP VLEQLADYTE QRQQSRQKIQ LALLYPVILM VASLAIVGFL LGYVVPDVVR VFIDSGQTLP LLTRVLIGVS DWVKAWGALA FVAAIGGVIG FRYALRKDAF RERWHGFLLR VPLVGRLVRS TDTARFASTL AILTRSGVPL VEALAIAAEV IANRIIRNEV VKAAQKVREG ASLTRSLEAT GQFPPMMLHM IASGERSGEL DQMLARTARN QENDLAAQIG LMVGLFEPFM LIFMGAVVLV IVLAILLPIL SLNQLVG singleParticle particle 7.5 NEGATIVE Uranyl Acetate JEOL 1400 FLOOD BEAM BRIGHT FIELD LAB6 120 TVIPS TEMCAM-F416 (4k x 4k) 2.0 1 20.0 FSC 0.143 CUT-OFF 2522 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_0045.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 100 100 100 0 0 0 100 100 100 229.0 229.0 229.0 90.0 90.0 90.0 X Y Z -0.015363826 0.05337277 0.0005494566 0.005350336 2.29 2.29 2.29 0.0175 EMDB Final map after 3D refinement (Relion) low-pass filtered to 2nm.