2018-09-12 REL PDBe PDBe PDBe 2018-05-26 2018-08-22 2018-09-05 2018-09-12 Structure of activated transcription complex Pol II-DSIF-PAF-SPT6 (Map B, NGN/SPT4 selected particles) Vos SM Farnung L Boehning M Linden A Wigge C Urlaub H Cramer P Vos SM Farnung L Boehning M Wigge C Linden A Urlaub H Cramer P Structure of activated transcription complex Pol II-DSIF-PAF-SPT6. Nature UK 560 607 612 2018 30135578 doi:10.1038/s41586-018-0440-4 1476-4687 0006 NATUAS EMD-0030 associated EM volume RNA Polymerase II-DSIF-PAF-SPT6 elongation complex (EC*) RNA Polymerase II-DSIF-PAF-SPT6 elongation complex (EC*) 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 23 1.257 RNA Polymerase II 1 1 2 3 4 5 6 7 8 9 11 10 12 Sus scrofa associated proteins 1 13 16 18 23 19 20 21 22 Homo sapiens Trichoplusia ni Nucleic acids 1 14 15 17 synthetic construct synthetic construct singleParticle particle 7.4 ETHANE 100 277 FEI VITROBOT MARK IV
10s wait prior to blotting, blotting 8.5s.
FEI TITAN KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 130000. FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 10.0 40.0 1 C1 3.34 FSC 0.143 CUT-OFF RELION 2.1 105854 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_0030.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 377.64 377.64 377.64 90.0 90.0 90.0 X Y Z -0.018119918 0.051605143 0.00018398488 0.0020904206 1.049 1.049 1.049 0.00576 AUTHOR Global refinement of NGN/SPT4 selected particles. emd_0030_msk_1.map emd_0030_additional.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 377.64 377.64 377.64 90.0 90.0 90.0 X Y Z -0.20202968 0.3599306 0.0005789361 0.0075578466 1.049 1.049 1.049 0.0386 AUTHOR Postprocessed map of global refinement for NGN/SPT4 selected particles with an applied B factor of -90.81 (Map B). emd_0030_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 377.64 377.64 377.64 90.0 90.0 90.0 X Y Z -0.026794693 0.066842325 0.00017557529 0.0032595522 1.049 1.049 1.049 0.0137 AUTHOR Half map 1 of global refinement for NGN/SPT4 selected particles (Map B). emd_0030_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 377.64 377.64 377.64 90.0 90.0 90.0 X Y Z -0.024141178 0.06594889 0.00017330523 0.00276221 1.049 1.049 1.049 0.0137 AUTHOR Half map 2 of global refinement for NGN/SPT4 selected particles (Map B).