2026-01-14 OBS RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY STRUCTURE_SUMMARY chem_comp chem_comp_atom chem_comp_bond em_admin entity pdbx_entity_nonpoly _chem_comp.name _chem_comp_atom.atom_id _chem_comp_bond.atom_id_1 _chem_comp_bond.atom_id_2 _chem_comp_bond.pdbx_stereo_config _chem_comp_bond.value_order _em_admin.last_update _entity.pdbx_description _pdbx_entity_nonpoly.name STRUCTURE_SUMMARY em_admin entity _chem_comp.name _em_admin.last_update _entity.pdbx_description DATABASE_REFERENCES citation citation_author em_admin _citation.title _em_admin.last_update OBSOLETE REPOSITORY OTHER citation citation_author em_admin em_supersede pdbx_database_PDB_obs_spr pdbx_database_status _em_admin.current_status _em_admin.last_update _pdbx_database_status.status_code OBSOLETE REPOSITORY EXPERIMENTAL_SUMMARY citation citation_author em_admin em_supersede pdbx_database_PDB_obs_spr _em_admin.current_status _em_admin.last_update RCSB RCSB 2024-10-17 2025-02-05 2025-02-05 2026-01-14 2026-01-14 2026-01-14 EMD-70618 National Institutes of Health/National Cancer Institute (NIH/NCI) CA231466 United States Cryo-EM structure of the C. neoformans lipid flippase Apt1-Cdc50 bound with butyrolactol A in the E2P state Duan HD Li H Cryptococcus neoformans, lipid flippase, P4-ATPase, Cdc50 protein, butyrolactol A, TRANSLOCASE-INHIBITOR complex Chen X Duan HD Hoy MJ Koteva K Spitzer M Guitor AK Puumala E Fiebig AA Hu G Yiu B Chou S Bian Z Choi Y Guo ABY Wang W Sun S Robbins N Averette AF Cook MA Truant R MacNeil LT Brown ED Kronstad JW Coombes BK Cowen LE Heitman J Li H Wright GD Butyrolactol A enhances caspofungin efficacy via flippase inhibition in drug-resistant fungi. Cell 2025 41478284 doi:10.1016/j.cell.2025.11.036 1097-4172 Chen X Duan HD Hoy MJ Koteva K Spitzer M Guitor AK Puumala E Fiebig AA Hu G Yiu B Chou S Bian Z Choi Y Ya Guo AB Wang W Sun S Robbins N Averette AF Cook MA Truant R MacNeil LT Brown ED Kronstad JW Coombes BK Cowen LE Heitman J Li H Wright GD Butyrolactol A potentiates caspofungin efficacy against resistant fungi via phospholipid flippase inhibition. Biorxiv US 2025 39829750 doi:10.1101/2025.01.06.630955 2692-8205 EMD-47334 consensus EM volume EMD-47335 focused EM volume 9dzu FULLOVERLAP EMDB EMD-47334 consensus EM volume EMDB EMD-47335 focused EM volume EMDB EMD-47338 associated EM volume
Cryo-EM structure of the C. neoformans lipid flippase Apt1-Cdc50 bound with butyrolactol A in the E2P state
 Apt1-Cdc50 heterodimer bound with butyrolactol A Apt1-Cdc50 heterodimer bound with butyrolactol A 0 1 2 Cryptococcus neoformans var. grubii H99 0.222 Transcription regulator Cryptococcus neoformans var. grubii H99 0.045937961 1 Saccharomyces cerevisiae LEVO MAIFNRKPKARLDGEPAPTEKEKVKWSKRPANTAFKQQRLKAWQPILTPKSVLPTLLIIGIIFAPIGALIVWGSGKVTTI TLDYTECDVDAPTDGSYQAMPNSAYQYDLATSSSVSESSIASPTWTFSNDSSREVGETARCEIEFEVPYDLGPGLFLYYK LTNYYQNHRRYSSSFDATQLIGDSRSLSQINGGNCKPITSRDGKPYYPCGLIANSLFNDTFPSVVLLNPTNGAQNQTYNF SESGIAWGGIKKNYASTLTYISPSDVLPPPNWALKYPNGYVDGFPNLREDEHFQVWMRVAALPTFRKLWARNDGEIMSQG RYRIVANMNYPVKQFSGTKSIVISTVSWIGGKQPFLGWAYIAAAILCVVLAVAGLIRHLVKPRKLGDMSLLSWNQPNANG LHHHHHHHHHH J9VW44 Phospholipid-transporting ATPase Cryptococcus neoformans var. grubii H99 0.17653460899999998 1 Saccharomyces cerevisiae LEVO MGASKPPLVPRSKKHNPSWLDRNIVKPLESLAPSKLFARRRSPPVPRSVFINEPLPSEYYDKKGKILRAHHFATNQNVTS KYTVITFIPKNLFEQFRRVANCFFLAISILQFFPKFSTISPGLVILPLIIVLAITALKDGYEDIKRHQADHRTNHAIVHV LGGQDYTNQNPMASKDKTFIPAIPLPKRRSKKAKKAEEEAALNMQGRSSSTENFAAEPVPGAEPRGQDELQRMRSQVSNW DEDPEAGDSPGELGWHRTIWEDVKVGDFVKIYENEQFPADIVICATSEEEDVAYIETKNLDGETNLKSRNGVPGLSHLNT AEACAKAHLCIDLDAPESNMFRLNGAVINLEEYDEDEQHPIHPITLETTMLRGCVLKNTAWVIGIIVYTGEDTKIIRNAG ATPSKRSKVEKQMNPQVIINLVILAAIAVVCAIVDHVNEVEWDRQQAYWMLFADTSGDNPNINGLVTFANAFITFQNIVP ISLYISIEAVRTIQAAFIYWDRDIKYKKDGVTTRTTARSWNLSDDLGQIEYIFSDKTGTLTQNAMIFRQCSVGGKIYTGD GLPPSHPTITHQHQPPPVHQHDDQDDPIAKSASESDDSDPKKISTEDPDEIKVTLPKEVLATFHDAELDKDLEAHDSEQS RILHGFFAVLGLCHTVLAAETEPGVIEYKAQSPDEAALVQSAADVGFVFRGRDHNILRMSTPFSDVSDEYELLHVLEFNS ARKRMSVILRKLDEDGRIFLLCKGADNVIFERLTKDSNQREMREKTDQDLQYFASEGLRTLCLAYRILDPQVYEQWAKEY HNATVALQDREERIESVSSSIERDLILLGATAIEDKLQDGVPDTISDLKRAGIKVWVATGDKLETAVAIGYTTNLLTKDT NLIVVREGRHSIGDQLREALEEFFGEDAGLRTTLSRIDSRRNSMDPPRLTRVNTGVRSLVGRDNGTRPGGFSLVIEGHAL AHCFDDEETEALLLALSTRCNTVICCRVSPLQKAQIVHLIKDNLGVMCLAIGDGANDVSMIQAADVGVGISGEEGLQAVN SSDYAIAQFRYLKRLLLVHGHWSYFRNSSMILNFFYKNIIGIGVLFWFMIYCGWSTTYVFAYVYLLFWNVFWTLVPVIAI GLFDRNIDDETLMALPELYRASREGKYFGLMRFAYYIFEGVYQSAVIYFFLNYTYVTTTARGDGYDVYMYEMSTTQAIGA VMVANLFSGLNIDAWTGWVWFAIWFGPFLIWVFTAVYSVIPPSSFYTGVYGNDVFLFRSAAYWFGWPFVTIIALLPRYLI KTFRQNIFPNDVDTMRLVRKYHPEVDLYNHPMLGGKLAPKKDEDESDYGEEPFDGPEGRRSSIKMANLRHSHGAFGRGDQ AGDMELGMGRKSLGNRPGLRSSMDSSRFGIHSGARGSTVDMSTGLEQPPSRGFGFTMEEGGVAIQRMQSRLSQTSSHASR SRWPRFNNNSSSSHPFETKPPSSMSKLRSRAGSILTRKRADTTDTRNSDDKSLSSPVKTGFFGRHMPGQNHGQHEGRSMG TPLKSETGRGDNWEEEELEDESLGRGFGVGQNMAPPEIPRMDYKDDDDKI J9VZ19 7.6.2.1 2-acetamido-2-deoxy-beta-D-glucopyranose 0.000221208 1 NAG BERYLLIUM TRIFLUORIDE ION 6.600700000000001e-05 1 BEF MAGNESIUM ION 2.4305e-05 1 MG (3R,4R,5S)-3,4-dihydroxy-5-[(1R,2R,3S,4S,5R,6R,8E,10E,14E,16Z)-1,2,3,4,5-pentahydroxy-6,20,20-trimethylhenicosa-8,10,14,16-tetraen-1-yl]oxolan-2-one 0.000526659 1 A1BD6 singleParticle particle 7.4 ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.3 1.7 GATAN K3 (6k x 4k) 60.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 2.72 FSC 0.143 CUT-OFF 639772 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_47338.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 248.40001 248.40001 248.40001 90.0 90.0 90.0 X Y Z 0.0 2.3154423 0.0015071733 0.0270967 0.828 0.828 0.828 0.01 AUTHOR Composite map