2025-03-05 OBS RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY OBSOLETE REPOSITORY EXPERIMENTAL_SUMMARY citation citation_author em_admin pdbx_database_PDB_obs_spr _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.current_status _em_admin.last_update OBSOLETE REPOSITORY STRUCTURE_SUMMARY citation citation_author em_admin pdbx_database_PDB_obs_spr pdbx_database_status pdbx_entry_details _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.current_status _em_admin.last_update _pdbx_database_status.status_code RCSB RCSB 2022-12-12 2023-01-11 2023-01-11 2025-03-05 2025-03-05 National Institutes of Health/National Cancer Institute (NIH/NCI) R01CA193578 United States National Institutes of Health/National Cancer Institute (NIH/NCI) R01CA227261 United States National Institutes of Health/National Cancer Institute (NIH/NCI) R01CA219700 United States SARS-CoV-2 Nucleocapsid dimer structure determined from COVID-19 patients Casasanta M Jonaid GM Kaylor L Luqiu W DiCecco L Solares M Berry S Kelly DF SARS-CoV-2, N protein, COVID-19, RNA binding protein, VIRAL PROTEIN Casasanta MA Jonaid GM Kaylor L Luqiu WY DiCecco LA Solares MJ Berry S Dearnaley WJ Kelly DF Retraction of: Structural Insights of the SARS-CoV-2 Nucleocapsid Protein: Implications for the Inner-workings of Rapid Antigen Tests. Microsc Microanal US 2025 39837351 doi:10.1093/mam/ozae127 1435-8115 Casasanta M Jonaid GM Kaylor L Luqiu W DiCecco L Solares M Berry S Dearnaley WJ Kelly DF Structural insights of the SARS-CoV-2 Nucleocapsid protein: Implications for the inner-workings of rapid antigen tests Microsc Microanal US 2022 doi:10.1093/micmic/ozac036/6957293 1435-8115 Casasanta MA Jonaid GM Kaylor L Luqiu WY Solares MJ Schroen ML Dearnaley WJ Wilson J Dukes MJ Kelly DF Microchip-based structure determination of low-molecular weight proteins using cryo-electron microscopy. Nanoscale UK 13 7285 7293 2021 33889923 doi:10.1016/j.apsb.2020.04.009 2040-3372 8fg2 FULLOVERLAP Nucleocapsid dimer is comprised of A chain and B chain Nucleocapsid dimer is comprised of A chain and B chain 0 1
For each monomer that comprises the dimer structure, residues 1-49 were fit into the map separately from residues 50 - 419.
Severe acute respiratory syndrome coronavirus 2 Nucleoprotein Homo sapiens 0.045689644999999994 2 LEVO MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSP DDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQ LPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQ QQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRI GMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADL DDFSKQLQQSMSSADSTQA P0DTC9 singleParticle particle 0.1 7.5
20 mM Tris (pH 7.5), 150 mM NaCl, 10 mM MgCl2, 10 mM CaCl2
 Homemade SILICON NITRIDE
Samples were incubated with Ni-NTA coated microchips for 1 minute prior to plunge freezing into liquid ethane.
 ETHANE 100 298 FEI VITROBOT MARK III
A Mark III Vitrobot was used to plunge samples into liquid ethane, operating at room temperature and 100% humidity with 3 - 4 seconds blot time.
Sample was enriched using Ni-NTA coated silicon nitride microchips
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.0 3.0 59000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN FEI FALCON III (4k x 4k) INTEGRATING 4 300 1.0 50.0 1 10000 1 C1 6.0 FSC 0.143 CUT-OFF cryoSPARC 10000 ANGULAR RECONSTITUTION cryoSPARC ANGULAR RECONSTITUTION RELION 1 10000.0 cryoSPARC emd_29072.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 91 77 84 0 0 0 84 77 91 117.6 107.799995 127.4 90.0 90.0 90.0 Z Y X -2.5431237 29.300049000000001 -0.000000000002932 1.0 1.4 1.4 1.4 0.207 AUTHOR SARS-CoV-2 Nucleocapsid dimer structure determined from COVID-19 patients RIGID BODY FIT REAL 100.0 emd_29072_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 91 77 84 0 0 0 84 77 91 117.6 107.799995 127.4 90.0 90.0 90.0 Z Y X -14.137299000000001 32.86356 -0.000000000021064 1.0 1.4 1.4 1.4 AUTHOR Half Map 2 emd_29072_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 91 77 84 0 0 0 84 77 91 117.6 107.799995 127.4 90.0 90.0 90.0 Z Y X -1.0085014 24.15814 0.000000000027885 1.0 1.4 1.4 1.4 AUTHOR Half Map 1