Focused refinement cryo-EM map of the A/B/C subunits of the T=3 lake sinai virus 1 (delta N-terminal 48 residues) virus-like particle at pH 6.5

2026-05-27 OBS PDBj INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY OBSOLETE REPOSITORY EXPERIMENTAL_SUMMARY citation citation_author em_admin em_supersede _citation.page_last _citation.pdbx_database_id_PubMed _citation.title _citation_author.identifier_ORCID _em_admin.current_status _em_admin.last_update DATA_UPDATED REPOSITORY DATA_UPDATED REPOSITORY PDBj PDBj 2022-05-04 2023-02-15 2023-02-15 2026-05-27 2026-05-27 2026-05-27 EMD-80888 Ministry of Science and Technology (MoST, Taiwan) Taiwan Focused refinement cryo-EM map of the A/B/C subunits of the T=3 lake sinai virus 1 (delta N-terminal 48 residues) virus-like particle at pH 6.5 Chen NC Wang CH Chen CJ Yoshimura M Guan HH Chuankhayan P Lin CC VIRUS LIKE PARTICLE Chen NC Wang CH Yoshimura M Yeh YQ Guan HH Chuankhayan P Lin CC Lin PJ Huang YC Wakatsuki S Ho MC Chen CJ Structures of honeybee-infecting Lake Sinai virus reveal domain functions and capsid assembly with dynamic motions. Nat Commun UK 14 545 545 2023 36726015 doi:10.1038/s41467-023-36235-3 2041-1723 EMDB EMD-33383 associated EM volume Lake Sinai virus 2 Lake Sinai virus 2 0 1 Lake Sinai virus 2 VIRUS-LIKE PARTICLE STRAIN false true Lake sinai virus 1 capsid protein Lake Sinai virus 2 LEVO MNSICSLYCLFRRVMPYSSEILSSTVYRPPFLATSGLFFTRDHYKFIIMNQQQINPAQRSLRPRAQSAPSRSARRRRNRRRRNPSTPAGTVALQPSRITRRVVSNLARRPLTITTAGLAWLRQYLNPMGPSTSSVSGFPDGSAVTTCIADYTNTFNISFPPREAIYCTGSNSDEKPVMLDAATYAKIDAWTKSDITLCILALPMLRNVVMIRLYASTPTAFTLSEGVPNFVQRFPNWSAFTTEGKVLNNGDSPGYIQSFVYLPNVDKHLSAARGFRLLSRGLTGIYTAPSLETQGFVTACQYLAEGALQTQTVGNDFVQSVEVNADKTVKNVNGKRLHYSGPPKFVFPLEGDNCAPSSLVETYHQAYQARAVDGFYMPILSSSRDNPFISPKPQPIAVFNRWYYRGCLDPVPASKVADGPSQYYYDLNVADDVAPLYNTGVVWMEGISSKFSLKLKTRTVIQYIPTSGSVLANFTRHEPTYDQVALDAADRIRNMMPHAYPAAYNDWGWLGDLLDSTLSMLPGIGTAYRFAKPLIKPAWNWLGGKVSDFFGNPVSRDGDIYFDAK singleParticle particle 7.5 ETHANE 100 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.25 2.72 GATAN K3 BIOQUANTUM (6k x 4k) 46.0 1 40418 CTFFIND PHASE FLIPPING ONLY Ab initial in cisTEM 2.58 FSC 0.143 CUT-OFF 40418 MAXIMUM LIKELIHOOD cisTEM PROJECTION MATCHING cryoSPARC emd_33383.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 539.4944 539.4944 539.4944 90.0 90.0 90.0 X Y Z -0.3979273 2.5558383 0.017175617 0.03744277 1.0537 1.0537 1.0537 0.2 AUTHOR ::::EMDATABANK.org::::EMD-33383:::: emd_33383_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 -256 -256 -256 512 512 512 539.4944 539.4944 539.4944 90.0 90.0 90.0 X Y Z -0.21242708 0.6236921 0.0035964688 0.029796664 1.0537 1.0537 1.0537 AUTHOR ::::EMDATABANK.org::::EMD-33383:::: emd_33383_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 -256 -256 -256 512 512 512 539.4944 539.4944 539.4944 90.0 90.0 90.0 X Y Z -0.20370656 0.60114014 0.0035697196 0.029761327 1.0537 1.0537 1.0537 AUTHOR ::::EMDATABANK.org::::EMD-33383::::